Different effects of two brassinosteroids on growth, auxin and cytokinin content in tobacco callus tissue

Gaudinová, Alena; Süssenbeková, Hana; Vojtěchová, Martina; Kamínek, M.; Eder, J.; Kohout, Ladislav

doi:10.1007/bf00024171

Cited by 30 publications

(14 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At the biochemical level, BRs have been demonstrated to change endogenous cytokinin concentrations in plant species. For example, when provided via a culture medium containing a growth-limiting level of auxin, 24-epibrassinolide increased the endogenous predominant cytokinins N-6-( -2-isopentenyl) adenine and trans-zeatin in tobacco (Nicotiana tabacum) callus tissue (Gaudinova et al 1995). Thus, BRs might stimulate branch elongation via a BR-driven enhancement of the endogenous levels of cytokinins.…”

Section: Discussionmentioning

confidence: 98%

Brassinosteroid-stimulated branch elongation in the marubakaido apple rootstock

Pereira-Netto

Cruz-Silva

Schaefer

et al. 2005

Trees

View full text Add to dashboard Cite

Brassinosteroids (BRs) comprise a specific class of low-abundance plant steroids now recognized as a new class of phytohormones. In this paper, we demonstrate that a fluoro derivative of 28-homocastasterone (5F-HCTS) stimulates branch elongation in in vitro-grown shoots of Malus prunifolia, the marubakaido apple rootstock. In addition to that, we show that this BR-stimulated branch elongation is paralleled by an increase in ethylene release. However, either the presence of 1-amino-cyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene in higher plants, in the culture medium or an ethylene-enriched atmosphere resulted in inhibition of branch elongation, indicating that the stimulation of branch elongation observed for 5F-HCTS-treated shoots in this study was not, at least directly, related to the BR-induced enhancement in ethylene release rate. Besides its positive effect on the marubakaido shoot growth, i.e. branch elongation, the 5F-HCTS-driven enhancement of branch elongation found in this study is potentially useful to improve micropropagation techniques for other plant species as well, especially woody species, in which branch elongation is typically a constraint for efficient micropropagation.

show abstract

Section: Discussionmentioning

confidence: 98%

Brassinosteroid-stimulated branch elongation in the marubakaido apple rootstock

Pereira-Netto

Cruz-Silva

Schaefer

et al. 2005

Trees

View full text Add to dashboard Cite

show abstract

“…BRs also interact with other plant hormones to modulate various plant stress responses. Interactions of BRs with auxins (Katsumi, 1985;Gaudinova et al, 1995;Halliday, 2004), gibberellins (Mayumi and Shiboaka, 1995), ABA (Kurepin et al, 2008) and cytokinins (Gaudinova et al, 1995) have been recorded to regulate a variety of physiological and plant stress responses.…”

Section: Brassinosteriodsmentioning

confidence: 99%

Epibrassinolide regulated synthesis of polyamines and auxins in Raphanus sativus L. seedlings under Cu metal stress

Pal

Bhardwaj

Gupta³

et al. 2009

Braz. J. Plant Physiol.

View full text Add to dashboard Cite

The effects of various conc of (10 -11 , 10 -9 and 10 -7 M) 24-epibrassinolide (24-epiBL) on polyamines and auxins contents was analyzed in 7days old seedlings of Raphanus sativus L. cv. Pusa chetki under copper metal stress. Cu metal (0.2mM) treatment significantly increased endogenous levels of putrescine, spermidine and spermine when compared with control values. Cadaverine content got decreased under metal stress. Endogenous polyamines showed varying levels in seedlings when applying 24-epiBL treatment only. Maximum increase was found in putrescine, cadaverine and spermidine in 10 -11 , 10 -9 and 10 -7 M conc of 24-epiBL respectively when applied alone to R. sativus seedlings. However, metal treatment supplemented with 24-epiBL revealed small increase in polyamines. Putrescine and cadaverine got enhanced in combination of Cu metal and 10 -7 M (24-epiBL) in comparison to metal treatment alone, whereas decrease in spermidine and spermine levels were recorded in combinations of Cu metal and 10 -9 and 10 -7 M 24-epiBL respectively. Endogenous contents of Indole-3-acetic acid (IAA) and Naphthalene acetic acid (NAA) were significantly affected by 24-epiBL when applied alone or along with Cu metal. Both free and bound forms of NAA were not detected in untreated seedlings, but were recorded in seedlings treated with metal stress. Decreased IAA contents observed in metal treatment got enhanced significantly in seedlings with 24-epiBL supplementation. To the best of our knowledge the present investigation is the first one to explain the effects of 24-epiBL on endogenous contents of polyamines, indole acetic acid and naphthalene acetic acid under copper metal stress.

show abstract

“…Several authors have observed stimulation of growth elongation or promotion of cell division and differentiation after treatment with brassinosteroids (Gaudinová et al 1995;Clouse 1996;Bao et al 2004). Brassinosteroids can behave either as auxins or as cytokinins and the elucidation of the behavior can be accomplished by experimental morphology using model plants.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of biological activity of new synthetic brassinolide analogs

et al. 2009

View full text Add to dashboard Cite

The responses of plants to exogenous treatment with new synthetic brassinosteroids (BRs) were assessed and compared with the activity of natural 24-epibrassinolide (24-EPI). Morphological experiments on plants of pea and flax showed that the boundary between stimulatory and inhibitory concentrations of individual BRs and 24-EPI used is very narrow and differs also with the plant species. Moreover brassinosteroids can exhibit effects similar to various other plant hormones. This was proven also in our experiments, where auxin, anti-auxin and cytokinin like effects were achieved by BRs application. One of the explanations of the different morphological effects could be the influence of brassinosteroid application on the level of endogenous hormones. There are changes in the levels of indole-3-acetic acid, 6-benzylaminopurine, trans-zeatin and dihydrozeatin in rape and wheat plants caused by BR 4 and 24-EPI application, but there is no general trend explaining unequivocally their influence. The fact that all tested BRs significantly increased the dry weight accumulation in comparison with non-treated reference rape plants can be accounted for the known BRs characteristics to avoid biotic stresses.

show abstract

Different effects of two brassinosteroids on growth, auxin and cytokinin content in tobacco callus tissue

Cited by 30 publications

References 23 publications

Brassinosteroid-stimulated branch elongation in the marubakaido apple rootstock

Brassinosteroid-stimulated branch elongation in the marubakaido apple rootstock

Epibrassinolide regulated synthesis of polyamines and auxins in Raphanus sativus L. seedlings under Cu metal stress

Evaluation of biological activity of new synthetic brassinolide analogs

Contact Info

Product

Resources

About