Different distribution of antimicrobial resistance genes and virulence profiles of Staphylococcus aureus strains isolated from clinical mastitis in six countries

Monistero, Valentina; Barberio, A.; Biscarini, Filippo; Cremonesi, Paola; Castiglioni, Bianca; Graber, H.U.; Bottini, Enriqueta; Ceballos-Márquez, Alejandro; Kroemker, Volker; Petzer, Inge-Marié; Pollera, C.; Santisteban, Carlos; Santos, Marcos Veiga dos; Bronzo, V.; Piccinini, R.; Cocchi, Monia; Moroni, P.

doi:10.3168/jds.2019-17141

Cited by 33 publications

(28 citation statements)

References 62 publications

(45 reference statements)

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“…High resistance levels to penicillin and other βlactams among mastitis-causing staphylococci has been described [35][36][37]. In contrast, a lower resistance to penicillin in staphylococci (0-20%) has also been reported in some European countries [38,39] and Canada [7]. Higher resistance rates are likely due to the wide-spread use of penicillin in the treatment of mastitis in dairy cows as observed in the study farms and as evidenced by other previous studies in Kenya [27,40] Further, changes overtime, spatial sampling, differences in antibiotic use and practices might explain discrepancies in resistance levels between regions [33,41].…”

Section: Discussionmentioning

confidence: 99%

“…This higher resistance could be due to the fact that penicillin is still the first-line drug of choice for treatment of mastitis in Kenya [27,34]. Routine culture and identification, coupled with antibiotic sensitivity testing, should be adopted before treatment with antimicrobials to avoid selection pressure of antimicrobial resistant S. aureus [37,39].…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Resistance Profiles and Genes of Staphylococci Isolated from Mastitic Cow’s Milk in Kenya

et al. 2021

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Increasing numbers of potentially zoonotic multidrug-resistant (MDR) staphylococci strains, associated with mastitis in dairy cows, are being reported globally and threaten disease management in both animal and human health. However, the prevalence and antimicrobial resistance profiles of these strains, including methicillin-resistant staphylococci (MRS), in Kenya is not well known. This study investigated the drug resistance profiles and genes carried by 183 staphylococci isolates from 142 dairy cows representing 93 farms recovered from mastitis milk of dairy cows in two selected counties in Kenya. Staphylococci isolates were characterized by phenotypic characteristics, polymerase chain reaction (PCR) amplification, partial sequencing and susceptibility testing for 10 antimicrobial drugs. Detection of seven resistance genes to the various antimicrobial drugs was conducted using PCR. Overall, phenotypic resistance among the staphylococci ranged between 66.1% for ampicillin and 3.5% for fluoroquinolones. Twenty-five percent (25%) of S. aureus and 10.8% of the coagulase-negative staphylococci (CoNS) isolates, were methicillin-resistant staphylococci phenotypically (defined as resistance to cefoxitin disk diffusion). The most common genes found in S. aureus and CoNS were blaZ and strB at 44.3% and 26%, and 78% and 50%, respectively. MDR was observed in 29.67% and 16.3% of S. aureus and CoNS, respectively. These findings pose a threat to bovine mastitis treatment and management as well as human health.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Profiles and Genes of Staphylococci Isolated from Mastitic Cow’s Milk in Kenya

et al. 2021

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“…Reported frequency of occurrence of the blaZ is variable depending on the region. The data for the USA and Germany are similar, as they are at the level of 41.2% and 47.1%, respectively [7]. On the other hand, information showing this frequency in Brazil is 74.07% [8].…”

Section: Discussion Of the Problemmentioning

confidence: 64%

Properties of coagulase-positive staphylococcal cells that make it difficult to diagnose and treat mastitis in cows

Grzeczka

Niewitecki

2021

Medical Journal of Cell Biology

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Coagulase-positive staphylococci are a group of bacteria that, among other things, cause inflammation of the udder in cows (mastitis). The identification of the species of staphylococcus causing the inflammation is crucial for the success of the treatment process due to the individual characteristics of the strains and even the characteristics of the bacterial population in the herd. However, through evolution, staphylococci have developed a number of processes that make species identification difficult. Knowledge of the actual cause of inflammation enables the selection of appropriate therapy, but despite advanced diagnostic techniques, erroneous results still occur. In this review, we present the current knowledge of mainly resistance mechanisms and how they affect the drug resistance of microorganisms. We also highlight the difficulties in the diagnosis and treatment of bovine mastitis.

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“…Similarly, as in South Africa, most isolates from the United States (New York State), Argentina and Germany showed resistance to spiramycin (Monistero et al 2020). In contrast to isolates from South Africa, Ethiopia and the United States of America, spiramycin and/or penicillin were not effective in 58% of Italian isolates (Monistero et al 2020). Although the Ethiopian study did not test for spiramycin resistance, very high resistance to penicillin was shown (Kalayu et al 2020).…”

Section: Staphylococcus Aureus Resistance In South Africa Compared To That Of Other Countriesmentioning

confidence: 92%

“…Cosandey et al ( 2016 ) described S. aureus genotype B (GTB) only in Italy, whilst S. aureus genotype R (CLR) and S. aureus genotype C (CLC) were identified in many places in Europe, America and Africa. Genotype CLR, which represents a large cluster containing 13 variants, was detected in all eight countries studied except for Brazil (Monistero et al 2020 ). Cosandey et al ( 2016 ) have shown that S. aureus CLR and CLC clusters are exclusive to dairy cattle and the spread thereof probably started many years ago, when cattle were moved from Europe to other countries (Monistero et al 2018 ).…”

Section: Review Findingsmentioning

confidence: 99%

The spread and antimicrobial resistance of Staphylococcus aureus in South African dairy herds – A review

Karzis

Petzer

Naidoo

et al. 2021

Onderstepoort Journal of Veterinary Research

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Staphylococcus aureus is internationally recognised as a principal agent of mastitis and the foremost reason for economic loss in the dairy industry. The limited data available on organism-specific antibiotic resistance surveillance in dairy cattle have stimulated the need for such a review article. The objective of this study was to review relevant literature on antimicrobial resistance of mastitis-causing staphylococci isolated from dairy cows in South Africa compared to other countries. Factors relating to the incidence of mastitis and treatment strategies in terms of the One Health concept and food security were included. The Web of Science (all databases) and relevant websites were used, and articles not written in English were excluded. The incidence of mastitis varied between South Africa and other countries. Antimicrobial resistance patterns caused by S. aureus also varied in regions within Southern Africa and those of other countries although some similarities were shown. Antimicrobial resistance differed between S. aureus bacteria that were maltose positive and negative (an emerging pathogen). The results highlighted the importance of the availability of organism-specific surveillance data of the incidence of mastitis and antibiotic resistance for specific countries and within similar climatic conditions. Accurate knowledge about whether a specific pathogen is resistant to an antibiotic within a certain climate, country, area or farm should reduce the incidence of unnecessary or incorrect treatment with antibiotics. This should enable dairy farmers to deal with these organisms in a more effective manner. Therefore such research should be ongoing.

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Different distribution of antimicrobial resistance genes and virulence profiles of Staphylococcus aureus strains isolated from clinical mastitis in six countries

Cited by 33 publications

References 62 publications

Antimicrobial Resistance Profiles and Genes of Staphylococci Isolated from Mastitic Cow’s Milk in Kenya

Antimicrobial Resistance Profiles and Genes of Staphylococci Isolated from Mastitic Cow’s Milk in Kenya

Properties of coagulase-positive staphylococcal cells that make it difficult to diagnose and treat mastitis in cows

The spread and antimicrobial resistance of Staphylococcus aureus in South African dairy herds – A review

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