Different developmental functions for calmodulin in dictyostelium: Trifluoperazine and R24571 both inhibit cell and pronuclear fusion but enhance gamete formation*1

Lydan, Michael A.; O’Day, Danton H.

doi:10.1016/0014-4827(88)90377-1

Cited by 37 publications

(26 citation statements)

References 13 publications

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“…It is necessary for spore swelling (5) and is required for cell and pronuclear fusion (6). Localization studies revealed that CaM is enriched on the membranes of the osmoregulatory system, i.e., the contractile vacuole (7).…”

Section: Camentioning

confidence: 99%

Production of Reagents and Optimization of Methods for Studying Calmodulin-Binding Proteins

Ulbricht

Soldati

1999

Protein Expression and Purification

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Owing to subtle but potentially crucial structural and functional differences between calmodulin (CaM) of different species, the biochemical study of low-affinity CaM-binding proteins from Dictyostelium discoideum likely necessitates the use of CaM from the same organism. In addition, most of the methods used for identification and purification of CaM-binding proteins require native CaM in nonlimiting biochemical quantities. The gene encoding D. discoideum CaM has previously been cloned allowing production of recombinant protein. The present study describes the expression of D. discoideum CaM in Escherichia coli and its straightforward and rapid purification. Furthermore, we describe the optimization of a complete palette of assays to detect as little as nanogram quantities of proteins binding CaM with middle to low affinities. Purified CaM was used to raise high-affinity polyclonal antibodies suitable for immunoblotting, immunofluorescence, and immunoprecipitation experiments. The purified CaM was also used to optimize a specific and sensitive nonradioactive CaM overlay assay as well as to produce a high-capacity CaM affinity chromatography matrix. The effectiveness of this methods is illustrated by the detection of potentially novel D. discoideum CaM-binding proteins and the preparatory purification of one of these proteins, a short tail myosin I.

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Section: Camentioning

confidence: 99%

Production of Reagents and Optimization of Methods for Studying Calmodulin-Binding Proteins

Ulbricht

Soldati

1999

Protein Expression and Purification

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“…Ca 2+ -calmodulin complex binds the target proteins, acting positively on zygote formation (Lydan & O'Day, 1988b). Concerning PKC, phorbol esters such as 12O-tetradecanoylphorbol-13-acetate (TPA), potent activators of PKC, have been reported to enhance the formation of zygotes in both D. discoideum and Dm 7.…”

Section: Inductive Signal Transduction Pathways For Zygote Formationmentioning

confidence: 99%

“…Since these protein kinase inhibitors are effective at the developmental stage when the cells acquired fusion competence, it is most likely that they may inhibit the process of gamete formation. In this way, calmodulin dependent protein kinases are involved in both inductive and inhibitory signal transduction pathways (Lydan & O'Day, 1988b). The inductive and inhibitory signal transduction pathways involved in zygote formation are summarized in Fig.…”

Section: Inhibitory Signal Transduction Pathways For Zygote Formationmentioning

confidence: 99%

Regulatory Mechanism in Sexual and Asexual Cycles of Dictyostelium

Amagai¹

2012

Advances in Selected Plant Physiology Aspects

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“…From several pharmacological studies, cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) have been shown to function as an inhibitory factor for zygote formation (Suzuki et al 1992;Kawai et al 1993). On the other hand, Lydan & O'Day (1988, 1993a It has been reported that the Ca 2+ -and PKC-mediated signaling pathway is involved in cell fusion during myogenesis (Shainberg et al 1969;Paterson & Strohman 1972;David et al 1990). The cell fusion as observed in the fertilization and myogenesis may share the Ca 2+ -and PKC-mediated signaling pathway.…”

Section: Perspectivementioning

confidence: 99%

Ethylene as a potent inducer of sexual development

Amagai¹

2011

Dev Growth Differ

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A novel and critical function of ethylene, a potent plant hormone, has been well documented in Dictyostelium, because it leads cells to the sexual development (macrocyst formation) by inducing zygote formation. Zygote formation (sexual cell fusion) and the subsequent nuclear fusion are the characteristic events occurring during macrocyst formation. A novel gene, zyg1 was found to be predominantly expressed during the sexual development, and its enforced expression actually induces zygote formation. As expected, the zygote inducer, ethylene enhances the expression of zyg1. Thus the function of ethylene has been verified at all of individual (macrocyst formation), cellular (zygote formation), and molecular levels (zyg1 expression). Based on our recent studies concerning the behavior and function of the zyg1 product (ZYG1 protein), the signal transduction pathways involved in zygote formation are proposed in this review.

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Different developmental functions for calmodulin in dictyostelium: Trifluoperazine and R24571 both inhibit cell and pronuclear fusion but enhance gamete formation*1

Cited by 37 publications

References 13 publications

Production of Reagents and Optimization of Methods for Studying Calmodulin-Binding Proteins

Production of Reagents and Optimization of Methods for Studying Calmodulin-Binding Proteins

Regulatory Mechanism in Sexual and Asexual Cycles of Dictyostelium

Ethylene as a potent inducer of sexual development

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