Different cellular fatty acid pattern and gene expression of planktonic and biofilm state Listeria monocytogenes under nutritional stress

Wang, Yuan; Wu, Yi-nan; Niu, Hongmei; Liu, Yangtai; Ma, Yue; Wang, Xiang; Li, Zhuosi; Dong, Qingli

doi:10.1016/j.foodres.2023.112698

Cited by 6 publications

(2 citation statements)

References 62 publications

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“…To adapt to a nutrient-stress environment lacking fatty acids, the expression levels of chemotaxis genes (cheA/Y) of Listeria monocytogenes were significantly up-regulated by 28.44 and 4.14 times, respectively. The overexpression of chemotaxis genes enabled L. monocytogenes to promote biofilm formation, which is an adaptive mechanism for pathogens to cooperate to overcome nutrient deficiency [49]. For V. harveyi in this study, the host muscle is rich in amino acids, which induce bacteria to synthesize many MCPs, flagella, and chemotaxis-associated proteins as reserves to prepare for further proliferation and expansion to the optimal sites of the host.…”

Section: Discussionmentioning

confidence: 89%

Amino Acid-Induced Chemotaxis Plays a Key Role in the Adaptation of Vibrio harveyi from Seawater to the Muscle of the Host Fish

Zhang,

Yan

et al. 2024

Microorganisms

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Vibrio harveyi is a normal flora in natural marine habitats and a significant opportunistic pathogen in marine animals. This bacterium can cause a series of lesions after infecting marine animals, in which muscle necrosis and ulcers are the most common symptoms. This study explored the adaptation mechanisms of V. harveyi from the seawater environment to host fish muscle environment. The comprehensive transcriptome analysis revealed dramatic changes in the transcriptome of V. harveyi during its adaptation to the host fish muscle environment. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, flagellar assembly, oxidative phosphorylation, bacterial chemotaxis, and two-component systems play crucial roles in V. harveyi’s adaptation to host fish muscle. A comparison of biological phenotypes revealed that V. harveyi displayed a significant increase in flagellar length, swimming, twitching, chemotaxis, adhesion, and biofilm formation after induction by host fish muscle, and its dominant amino acids, especially bacterial chemotaxis induced by host muscle, Ala and Arg. It could be speculated that the enhancement of bacterial chemotaxis induced by amino acids plays a key role in the adaptation of V. harveyi from seawater to the muscle of the host fish.

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Section: Discussionmentioning

confidence: 89%

Amino Acid-Induced Chemotaxis Plays a Key Role in the Adaptation of Vibrio harveyi from Seawater to the Muscle of the Host Fish

Zhang,

Yan

et al. 2024

Microorganisms

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“…Kadam et al (2013) observed the highest L. monocytogenes biofilm formation in nutrient-poor media, such as Hsiang-Ning Tsai medium, whereas nutrient-rich media such as brain-heart infusion (BHI) broth and TSB resulted in the lowest biofilm biomass on polystyrene surfaces. Compared to the undiluted TSB supplied with yeast extract (TSBYE), a 10-fold diluted TSBYE increased the number of L. monocytogenes in 2-day-old biofilms on SS from 4 to 7 log CFU/cm 2 (Wang et al, 2023). On the contrary, the biovolume of L. monocytogenes biofilms on polystyrene surfaces was reduced in a nutrient-poor environment, as the biovolume was ∼5 × 10 5 μm 3 in 2-day-old biofilms grown in 10-fold diluted TSB compared to ∼8 × 10 5 μm 3 in full-strength TSB (Guilbaud et al, 2015).…”

Section: Growth Mediummentioning

confidence: 98%

Comprehensive strategies for controlling Listeria monocytogenes biofilms on food‐contact surfaces

Hua,

Zhu

2024

Comp Rev Food Sci Food Safe

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Listeria monocytogenes biofilms formed on food‐contact surfaces within food‐processing facilities pose a significant challenge, serving as persistent sources of cross‐contamination. In this review, we examined documented cases of foodborne outbreaks and recalls linked to L. monocytogenes contamination on equipment surfaces and in the food production environment, provided an overview of the prevalence and persistence of L. monocytogenes in different food‐processing facilities, and discussed environmental factors influencing its biofilm formation. We further delved into antimicrobial interventions, such as chemical sanitizers, thermal treatments, biological control, physical treatment, and other approaches for controlling L. monocytogenes biofilms on food‐contact surfaces. This review provides valuable insights into the persistent challenge of L. monocytogenes biofilms in food processing, offering a foundation for future research and practical strategies to enhance food safety.

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Airborne survival and stress response in Listeria monocytogenes across different growth temperatures

Yong,

Lee,

Kang

2024

Journal of Hazardous Materials

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Different cellular fatty acid pattern and gene expression of planktonic and biofilm state Listeria monocytogenes under nutritional stress

Cited by 6 publications

References 62 publications

Amino Acid-Induced Chemotaxis Plays a Key Role in the Adaptation of Vibrio harveyi from Seawater to the Muscle of the Host Fish

Amino Acid-Induced Chemotaxis Plays a Key Role in the Adaptation of Vibrio harveyi from Seawater to the Muscle of the Host Fish

Comprehensive strategies for controlling Listeria monocytogenes biofilms on food‐contact surfaces

Airborne survival and stress response in Listeria monocytogenes across different growth temperatures

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