Different breeds, different blood: Cytometric analysis of whole blood cellular composition in chicken breeds

Bílková, Barbora; Bainová, Zuzana; Janda, Jozef; Zita, Lukáš; Vinkler, Michal

doi:10.1016/j.vetimm.2017.05.001

Cited by 21 publications

(13 citation statements)

References 41 publications

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“…In this regard, it can be stated that the comparison of values of leukogram found by the traditional manual enumeration based on morphological characteristics using a microscope with their values, which were determined by the automatic determination using an analyzer, can be sometimes greatly misleading. Such a comparison can affect negatively Boever et al 2010;Seliger et al 2012;Bílková et al 2017). Besides, our results of the differential leukocyte counts in Cherry Valley hybrid ducks showed that LYM are the most abundant type of leukocytes, which is in accordance with the previous findings of Li et al (2012) and Eze et al (2014).…”

Section: Discussionsupporting

confidence: 92%

Haematological indicators in hybrid mallard ducks (Anas platyrhynchos) with regard to the use of meal from whole white lupin seeds in their diet

et al. 2017

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The objective of our study was to assess the effect of replacing soybean meal with the meal from whole white lupin seeds (Lupinus albus) of the Zulika variety in diets on selected haematological indicators in 40-day-old fattened hybrid mallard ducks. A total of 180 Cherry Valley ducks were divided into three groups (E1, E2, and control). The control group was fed a diet containing soybean meal. Soybean meal replaced with 50% and 100% meal of white lupin seeds were used in group E1 and group E2, respectively. At the end of the fattening, 12 ducks (6 males and 6 females) were randomly selected from each group for a haematological examination. From the result of this study, it is clear that the effect of the diet was found only on the slightly varying number of white blood cells and on the proportion of monocytes. Ducks of group E2 showed a slight increase in the total number of leukocytes which was accompanied by a decrease in the percentage share of monocytes (P < 0.05). Based on the results, it can be claimed that the replacement of soybean meal with meal from the Zulika variety of whole white lupin seeds in the diet did not have a negative effect on the determined blood indicators. Therefore, whole white lupin seeds were successfully used as the important protein component of the diet for fattening hybrid mallard ducks.

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Section: Discussionsupporting

confidence: 92%

Haematological indicators in hybrid mallard ducks (Anas platyrhynchos) with regard to the use of meal from whole white lupin seeds in their diet

et al. 2017

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“…There is published evidence that the heterophil:lymphocyte ratio varies between birds and is highly heritable (Campo and Davila, 2002). It also varies greatly amongst chicken breeds (Bilkova et al, 2017) and avian species (Minias, 2019). We did not observe any corresponding changes in circulating myeloid cells in these birds ( Figure 3 ) so this phenomenon appears specific to the spleen.…”

Section: Resultsmentioning

confidence: 99%

Analysis of the Progeny of Sibling Matings Reveals Regulatory Variation Impacting the Transcriptome of Immune Cells in Commercial Chickens

et al. 2019

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There is increasing recognition that the underlying genetic variation contributing to complex traits influences transcriptional regulation and can be detected at a population level as expression quantitative trait loci. At the level of an individual, allelic variation in transcriptional regulation of individual genes can be detected by measuring allele-specific expression in RNAseq data. We reasoned that extreme variants in gene expression could be identified by analysis of inbred progeny with shared grandparents. Commercial chickens have been intensively selected for production traits. Selection is associated with large blocks of linkage disequilibrium with considerable potential for co-selection of closely linked “hitch-hiker alleles” affecting traits unrelated to the feature being selected, such as immune function, with potential impact on the productivity and welfare of the animals. To test this hypothesis that there is extreme allelic variation in immune-associated genes we sequenced a founder population of commercial broiler and layer birds. These birds clearly segregated genetically based upon breed type. Each genome contained numerous candidate null mutations, protein-coding variants predicted to be deleterious and extensive non-coding polymorphism. We mated selected broiler-layer pairs then generated cohorts of F2 birds by sibling mating of the F1 generation. Despite the predicted prevalence of deleterious coding variation in the genomic sequence of the founders, clear detrimental impacts of inbreeding on survival and post-hatch development were detected in only one F2 sibship of 15. There was no effect on circulating leukocyte populations in hatchlings. In selected F2 sibships we performed RNAseq analysis of the spleen and isolated bone marrow-derived macrophages (with and without lipopolysaccharide stimulation). The results confirm the predicted emergence of very large differences in expression of individual genes and sets of genes. Network analysis of the results identified clusters of co-expressed genes that vary between individuals and suggested the existence of trans-acting variation in the expression in macrophages of the interferon response factor family that distinguishes the parental broiler and layer birds and influences the global response to lipopolysaccharide. This study shows that the impact of inbreeding on immune cell gene expression can be substantial at the transcriptional level, and potentially opens a route to accelerate selection using specific alleles known to be associated with desirable expression levels.

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“…Precisely 1.0 ml of EDTA blood was mixed with 0.20 ml cellular fixant (Transfix®, MBL International), stored and shipped on wet ice to Cayman Analytical Laboratories (Ann Arbor, MI) for Heterophile to Lymphocyte ratio analysis using Flow Cytometry as described by Lentfer et al. (2015) and Bílková et al. (2017) ( Bílková et al., 2017 ; Lentfer et al., 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Effect of broiler genetics, age, and gender on performance and blood chemistry

Livingston¹,

Cowieson

Crespo

et al. 2020

Heliyon

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A total of 640 broilers were used to determine the effects of strain, sex, and age on hematology and blood chemistry using rapid detection devices. Day old chicks from two genetic lines of common fast-growing and high-yield broiler strains were sexed and allocated to 40 pens (16 birds per pen) containing either male or female and Ross or Cobb strains (n = 10). Venous blood was analyzed weekly using 2 broilers from each pen (n = 20) using the i-STAT® Alinity Handheld Clinical Analyzer, Zoetis Vetscan VS2, and iCheck™ Carotene devices at 14, 21, 28, 35, and 42 d, as well as growth performance. Post-mortem health tracking metrics were also recorded on 42 d. Broilers were deemed healthy based on posting data results and performed in accordance with industry standards with males presenting greater BW and reduced FCR than female broilers. Ross broilers displayed greater BW to 14 d with similar FCR compared with Cobb birds. Day of age had a highly significant impact on blood calcium, phosphorus, potassium, sodium, chloride, carotene, aspartate aminotransferase, creatine kinase, bile acids, uric acid, total protein, albumin, globulin, total carbon dioxide, hematocrit, and malondialdehyde. Male broilers had reduced blood sodium, chloride, carotene, uric acid, albumin, and increased total protein, glucose, and total carbon dioxide. Ross broilers had greater blood potassium, and sodium, as well as reduced uric acid, total protein, globulin, and malondialdehyde, compared with Cobb birds. These results demonstrated the effectiveness of point-of-care devices in measuring blood chemistry and hematology in modern broilers. These data can be utilized to determine normal healthy blood ranges in these types of broilers when accounting for strain, sex, and age.

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Different breeds, different blood: Cytometric analysis of whole blood cellular composition in chicken breeds

Cited by 21 publications

References 41 publications

Haematological indicators in hybrid mallard ducks (Anas platyrhynchos) with regard to the use of meal from whole white lupin seeds in their diet

Haematological indicators in hybrid mallard ducks (Anas platyrhynchos) with regard to the use of meal from whole white lupin seeds in their diet

Analysis of the Progeny of Sibling Matings Reveals Regulatory Variation Impacting the Transcriptome of Immune Cells in Commercial Chickens

Effect of broiler genetics, age, and gender on performance and blood chemistry

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