Different Biological Action of Oleic Acid in ALDHhigh and ALDHlow Subpopulations Separated from Ductal Carcinoma In Situ of Breast Cancer

Abstract

The mechanisms underlying breast cancer progression of ductal carcinoma in situ (DCIS) associated with fatty acids are largely unknown. In the present study, we compared the action of oleic acid (OA) on two human DCIS cell lines, MCF10DCIS.COM (ER/PR/HER2-negative) and SUM225 (HER2 overexpressed). OA led to a significant increase in proliferation, migration, lipid accumulation and the expression of lipogenic proteins, such as SREBP-1, FAS and ACC-1, in MCF10DCIS.COM cells but not SUM225 cells. The ALDHhigh subpopulation analyzed by the ALDEFLUOR assay was approximately 39.2±5.3% of MCF10DCIS.COM cells but was small (3.11±0.9%) in SUM225 cells. We further investigated the different biological action of OA in the distinct ALDHlow and ALDHhigh subpopulations of MCF10DCIS.COM cells. OA led to an increase in the expression of ALDH1A1, ALDH1A2 and ALDH1A3 in MCF10DCIS.COM cells. SREBP-1 and ACC-1 were highly expressed in ALDHhigh cells relative to ALDHlow cells, whereas FAS was higher in ALDHlow cells. In the presence of OA, ALDHhigh cells were more likely to proliferate and migrate and displayed significantly high levels of SREBP-1 and FAS and strong phosphorylation of FAK and AKT relative to ALDHlow cells. This study suggests that OA could be a critical risk factor to promote the proliferation and migration of ALDHhigh cells in DCIS, leading to breast cancer progression.

The Spatial Relationship of Malignant and Benign Breast Lesions with Respect to the Fat-Gland Interface on Magnetic Resonance Imaging

“…We assume that the TDLU near the fat-gland interface might have stem cell characteristics that are targeted by carcinogenic insults, leading to transformation and cancer2829. The interaction between the fat and gland tissue near the fat-gland interface should be important in both normal development and neoplasia3031. Further study is needed to investigate this hypothesis.…”

Óleo da polpa de quatro variedades de abacate: extração, rendimento, caracterização e avaliação da atividade antioxidante

“…Oleic acid is most commonly found in avocado pulp. There is evidence that this acid plays an important role in inhibiting the development of cancer cells because of its ability to eliminate the expression of HER2 (erbB-2), an oncogene well known for its involvement in the etiology, progression and metastasis of cancer (Kim et al, 2016;Carrillo, Cavia & Alonso-Torre, 2012).…”