Different biocompatibility of crystalline triamcinolone deposits on retinal cells in vitro and in vivo

Szurman, Peter; Sierra, Ana; Kaczmarek, Radosław; Jaissle, Gesine B.; Wallenfels-Thilo, Barbara; Grisanti, Salvatore; Lüke, Matthias; Bartz‐Schmidt, Karl Ulrich; Spitzer, Martin S.

doi:10.1016/j.exer.2007.03.003

Cited by 30 publications

(22 citation statements)

References 24 publications

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“…Many reported clinical experiences have demonstrated that intravitreal use of TA during vitrectomy does not affect the closure rate and visual function although conflicting laboratory data about TA toxicity to the retina and RPE have been presented. [20][21][22][23][24] TA-associated complications, such as elevated intraocular pressure, were not found in any of our cases. The three patients who developed a cataract during the follow-up period were most likely not related to the use of TA.…”

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confidence: 81%

Anatomical and visual outcome after vitrectomy with triamcinolone acedonide-assisted epiretinal membrane removal in highly myopic eyes with retinal detachment due to macular hole

Fang

Zheng

Weng

et al. 2008

Eye

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Purpose To determine the effectiveness of staining an epiretinal membrane (ERM) with triamicinolone acedonide (TA) during vitrectomy for a retinal detachment (RD) due to a macular hole (MH) in highly myopic eyes. Patients and methods Thirty-four highly myopic eyes (4À6.0 dioptres) of 34 patients with an RD caused by an MH underwent vitrectomy with TA-assisted ERM removal. The excised ERMs from five eyes were examined by transmission electron microscopy (TEM). The main outcome measures were the retinal status and best-corrected visual acuity (BCVA). The anatomic status of the MH was determined by optic coherence tomography (OCT) in 24 successfully retinal-reattached eyes. The follow-up periods ranged from 5 to 20 months. Results The granules of TA adhered to the ERM and the residual posterior hyaloid, which made them more visible and facilitated their removal. Tissues resembling the internal limiting membrane were not detected in any of the five excised tissues. The retinal reattachment rate was 88% after the first surgery. The mean log MAR BCVA significantly improved from 1.83 ± 0.50 (mean±SD) before surgery to 1.22±0.35 at the final follow-up (Po0.001, Wilcoxon signed rank test). OCT showed a normal concave appearance in 11 of 24 eyes (46%), but a central neural epithelial defect was present in 13 of 24 eyes (54%). Conclusions TA facilitates the complete removal of both the ERM and residual posterior hyaloid, thus ensuring high anatomical and visual success rates in highly myopic eyes with an RD due to MH.

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mentioning

confidence: 81%

Anatomical and visual outcome after vitrectomy with triamcinolone acedonide-assisted epiretinal membrane removal in highly myopic eyes with retinal detachment due to macular hole

Fang

Zheng

Weng

et al. 2008

Eye

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“…Even a short-term direct exposure to TA crystals caused rapidly progressive, apoptotic cell death. These findings suggest that epiretinal deposits might be critical in view of a local biocompatibility [58,59]. This TA-induced cytotoxicity seems to be strongly concentration dependent.…”

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confidence: 98%

“…The reason for this discrepancy is still unknown. Our recent studies showed evidence that the corticosteroid drug effect of TA is nontoxic, if there is no direct contact of the TA crystals with the cell surface [58,59]. …”

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confidence: 99%

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The Retinal Biocompatibility of Dyes in the ex vivo Model of the Isolated Superfused Vertebrate Retina

Lüke

Grisanti²,

Lüke³

2013

Ophthalmologica

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Background: Peeling of the internal limiting membrane or epiretinal membranes is a successful principle in macular surgery to achieve a functional benefit. Different dyes are used to facilitate the identification of intraocular tissues. The aim of our work was to investigate the retinal tolerance to the different dyes and their solvent carriers to provide valuable data for surgeons in handling for an optimal intraoperative use. Methods: Using the ex vivo model of the isolated superfused vertebrate retina technique, the effects of the dyes were tested on human and bovine retinal function. The retinas were perfused with an oxygen preequilibrated standard solution. The electroretinogram (ERG) was recorded using Ag/AgCl electrodes. After recording stable ERG amplitudes, the dyes brilliant blue G, indocyanine green, trypan blue, patent blue, triamcinolone and their solvent carriers were investigated. Results: Reductions of the ERG amplitudes were found for each tested dye. The effects after application of the dyes were dependent on time and concentration of the applied dyes, which were different for each dye. Conclusion: In part, the ERG has shown strong effects already after a short period of dye application. Surgeons who rely on the intraocular use of the dyes should keep in mind our findings, and the use of some dyes should be limited to selected cases. The well-considered use of the dyes by the surgeons could lead to a better functional outcome and avoid a possible harmful outcome of the surgery after mishandling.

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“…Enquanto que para se testar a sensibilidade de células retinianas a esteróides, a cultura primária de células de rato já tenha sido utilizadada 96 , a maioria dos artigos publicados lida com cultura de células imortalizadas, pela maior facilidade no manuseio e na obtenção do número desejado de células. 93,94,97,110,132,133 Em nosso estudo, optamos por testar duas diferentes linhagens imortalizadas de Detectamos em nosso experimento um aumento transitório da expressão de GFAP, associado a qualquer dose testada da medicação, que não foi observado quando o mesmo volume de solução salina balanceada foi injetado na cavidade vítrea do olho contralateral, excluindo-se desta forma um aumento da PIO Discussão como fator causal. O real significado da expressão transitória de GFAP após injeção intravítrea de TRI ainda não está elucidado e deve ser objeto de futuras pesquisas na área.…”

Section: Morfologiaunclassified

Efeito da aplicação de acetato de triancinolona sem preservativo (Triensence®) na retina: estudo morfológico, eletrorretinográfico e em cultura de células retinianas

Zacharias¹

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Different biocompatibility of crystalline triamcinolone deposits on retinal cells in vitro and in vivo

Cited by 30 publications

References 24 publications

Anatomical and visual outcome after vitrectomy with triamcinolone acedonide-assisted epiretinal membrane removal in highly myopic eyes with retinal detachment due to macular hole

Anatomical and visual outcome after vitrectomy with triamcinolone acedonide-assisted epiretinal membrane removal in highly myopic eyes with retinal detachment due to macular hole

The Retinal Biocompatibility of Dyes in the ex vivo Model of the Isolated Superfused Vertebrate Retina

Efeito da aplicação de acetato de triancinolona sem preservativo (Triensence®) na retina: estudo morfológico, eletrorretinográfico e em cultura de células retinianas

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