Different binding mechanisms of <i>Staphylococcus aureus</i> to hydrophobic and hydrophilic surfaces

Maikranz, Erik; Spengler, Christian; Thewes, Nicolas; Thewes, Alexander; Nolle, Friederike; Jung, Philipp; Bischoff, Markus; Santen, Ludger; Jacobs, Karin

doi:10.1039/d0nr03134h

Cited by 68 publications

(96 citation statements)

References 51 publications

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“…Their experiments show that E. coli cells have distinct patches on their surface and that the number of these patches defines adhesive strength of a cell; if no patches exist, a cell hardly adheres. However, our results -together with former studies -lead to a slightly different notion for Gram-positive S. aureus cells: Since the force-distance curves on all positions of the surface look similar, namely cup-shaped, S. aureus cells seem to have many adhesive molecules at almost every position of the cell wall, but the strength of adhesion has maxima at certain locations(15).…”

supporting

confidence: 41%

“…By reproducing these experimental results with Monte Carlo (MC) simulations according to a recent model of adhesion (15), we show that the adhesion capability of S. aureus is driven by adhesins organized in distinct patches of various number over the cell envelope. These results are important for the fabrication of new materials and the design of more precise models to describe bacterial adhesion.…”

Section: Introductionmentioning

confidence: 86%

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The adhesion capability ofStaphylococcus aureuscells is heterogeneously distributed over the cell envelope

Spengler

Glatz

Maikranz

et al. 2021

Preprint

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Understanding and controlling microbial adhesion is an important biomedical problem. However, many properties of the adhesion process of bacteria are still unknown, for example the distribution of adhesive strength over the cell wall. While a patchy colloid model for adhesion has been developed recently for Gram-negative Escherichia coli cells, a comparable model for Gram-positive cells is not known. Here, we use single-cell force spectroscopy to measure the adhesion of Staphylococcus aureus at different positions on tailored surfaces. We find a heterogenous distribution of the adhesion forces with varying degrees of intensity. By comparing these results to simulations, we obtain the distribution of adhesive strength on the cell wall: The cells have several distinct spots of high adhesion capability, similar to the patchy colloid model. We discuss implications of our results for the development of new materials and the design and analysis of future studies.

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supporting

confidence: 41%

Section: Introductionmentioning

confidence: 86%

The adhesion capability ofStaphylococcus aureuscells is heterogeneously distributed over the cell envelope

Spengler

Glatz

Maikranz

et al. 2021

Preprint

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“…2e and Table S1). This observation is rather common for SCFS studies with staphylococci 22,24,26,31,35 and probably due to cell-to-cell variations that occur even between individual cells sampled from the same cell culture and at the same time point.…”

Section: S Aureus Displays Comparable Adhesion Forces On Different Tmentioning

confidence: 92%

Human blood plasma factors affect the adhesion kinetics of Staphylococcus aureus to central venous catheters

Gunaratnam

Spengler

Trautmann

et al. 2020

Sci Rep

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Staphylococcus aureus is a common cause of catheter-related blood stream infections (CRBSI). The bacterium has the ability to form multilayered biofilms on implanted material, which usually requires the removal of the implanted medical device. A first major step of this biofilm formation is the initial adhesion of the bacterium to the artificial surface. Here, we used single-cell force spectroscopy (SCFS) to study the initial adhesion of S. aureus to central venous catheters (CVCs). SCFS performed with S. aureus on the surfaces of naïve CVCs produced comparable maximum adhesion forces on three types of CVCs in the low nN range (~ 2–7 nN). These values were drastically reduced, when CVC surfaces were preincubated with human blood plasma or human serum albumin, and similar reductions were observed when S. aureus cells were probed with freshly explanted CVCs withdrawn from patients without CRBSI. These findings indicate that the initial adhesion capacity of S. aureus to CVC tubing is markedly reduced, once the CVC is inserted into the vein, and that the risk of contamination of the CVC tubing by S. aureus during the insertion process might be reduced by a preconditioning of the CVC surface with blood plasma or serum albumin.

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“…From a force-distance curve it is possible to obtain values for the adhesion force (see fig. 1a) and rupture length and further detailed information on adhesinspecific unbinding events [46,32], of which the latter ones are not subject to this paper. For every bacterial cell on each surface, a set of 50 force-distance curves in different spots on the surface for each contact time was recorded and evaluated (see fig.…”

Section: Sample Characterizationmentioning

confidence: 99%

“…It is known that the surface chemistry, hydrophobicity and surface charge [31,32] or functional groups deposited on top of a surface influence the bacterial adhesion process. Such functional groups could be for instance proteins, such as silk proteins [33,34], or bodily fluids such as blood plasma [35,36] or salivary macromolecules [37,38,14].…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

Mischo

Faidt

McMillan

et al. 2021

Preprint

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Research into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For medical application of materials, particular attention has to be paid to biocompatibility, osseointegration and bacterial adhesion behavior. To understand their properties and behavior, experimental studies with natural materials such as teeth are strongly required. The results, however, may be highly case-dependent because natural surfaces have the disadvantage of being subject to wide variations, for instance in their chemical composition, structure, morphology, roughness, and porosity. A synthetic surface which mimics enamel in its performance with respect to bacterial adhesion and biocompatibility would, therefore, facilitate systematic studies much better. In this study, we discuss the possibility of using hydroxyapatite (HAp) pellets to simulate the surfaces of teeth and show the possibility and limitations of using a model surface. We performed single-cell force spectroscopy with single Staphylococcus aureus cells to measure adhesion-related parameters such as adhesion force and rupture length of adhesins binding to HAp and enamel. We also examine the influence of blood plasma and saliva on the adhesion properties of S. aureus. The results of these measurements are matched to water wettability, elemental composition of the samples and the change in the macromolecules adsorbed over time. We found that the adhesion properties of S. aureus were similar on both samples under all conditions: Significant decreases in adhesion strength were found equally in the presence of saliva or blood plasma on both surfaces. We therefore conclude that HAp pellets are a good alternative for natural dental material. This is especially true when slight variations in the physicochemical properties of the natural materials may affect the experimental series.

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Different binding mechanisms of Staphylococcus aureus to hydrophobic and hydrophilic surfaces

Abstract: A combination of force spectroscopic experiments and theoretical simulations reveals the molecular processes responsible for the adhesion of S. aureus.

Cited by 68 publications

References 51 publications

The adhesion capability ofStaphylococcus aureuscells is heterogeneously distributed over the cell envelope

The adhesion capability ofStaphylococcus aureuscells is heterogeneously distributed over the cell envelope

Human blood plasma factors affect the adhesion kinetics of Staphylococcus aureus to central venous catheters

Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

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