Different behaviour of fresh and cultured CD34<sup>+</sup> cells during immunomagnetic separation

Denning-Kendall, Patricia A.; Horsley, H; Donaldson, Craig; Bradley, Benjamin A.; Hows, JM

doi:10.1046/j.1365-2141.1999.01397.x

Cited by 12 publications

(12 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to the electrophysiological signs of encephalopathy (e.g., seizure and/or abnormal amplitude-integrated electroencephalogram),19 clinical signs of encephalopathy needed to be present in at least three of the following six categories: level of consciousness, spontaneous motor activity, tone, posture, primitive reflexes (Moro and sucking), and autonomic nervous system dysfunction 20…”

Section: Methodsmentioning

confidence: 99%

Human Umbilical Cord Blood CD34-Positive Cells as Predictors of the Incidence and Short-Term Outcome of Neonatal Hypoxic-Ischemic Encephalopathy: A Pilot Study

et al. 2017

View full text Add to dashboard Cite

Background and PurposeNeonatal hypoxic-ischemic encephalopathy (HIE) is one of the leading causes of neurological handicap in developing countries. Human umbilical cord blood (hUCB) CD34-positive (CD34+) stem cells exhibit the potential for neural repair. We tested the hypothesis that hUCB CD34+ stem cells and other cell types [leukocytes and nucleated red blood cells (NRBCs)] that are up-regulated during the acute stage of perinatal asphyxia (PA) could play a role in the early prediction of the occurrence, severity, and mortality of HIE.MethodsThis case-control pilot study investigated consecutive neonates exposed to PA. The hUCB CD34+ cell count in mononuclear layers was assayed using a flow cytometer. Twenty full-term neonates with PA and 25 healthy neonates were enrolled in the study.ResultsThe absolute CD34+ cell count (p=0.02) and the relative CD34+ cell count (CD34+%) (p<0.001) in hUCB were higher in the HIE patients (n=20) than the healthy controls. The hUCB absolute CD34+ cell count (p=0.04), CD34+% (p<0.01), and Hobel risk scores (p=0.04) were higher in patients with moderate-to-severe HIE (n=9) than in those with mild HIE (n=11). The absolute CD34+ cell count was strongly correlated with CD34+% (p<0.001), Hobel risk score (p=0.04), total leukocyte count (TLC) (p<0.001), and NRBC count (p=0.01). CD34+% was correlated with TLC (p=0.02).ConclusionshUCB CD34+ cells can be used to predict the occurrence, severity, and mortality of neonatal HIE after PA.

show abstract

Section: Methodsmentioning

confidence: 99%

Human Umbilical Cord Blood CD34-Positive Cells as Predictors of the Incidence and Short-Term Outcome of Neonatal Hypoxic-Ischemic Encephalopathy: A Pilot Study

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The expected yield from a 20–40 ml sample was 1 × 10 8 −1 × 10 9 cells. Cord blood and PBSC were processed in a similar way (Denning‐Kendall et al , 1999).…”

mentioning

confidence: 99%

Adult bone marrow is a rich source of human mesenchymal ‘stem’ cells but umbilical cord and mobilized adult blood are not

et al. 2003

View full text Add to dashboard Cite

Summary. In postnatal life, mesenchymal stem cells (MSC) self-replicate, proliferate and differentiate into mesenchymal tissues, including bone, fat, tendon, muscle and bone marrow (BM) stroma. Possible clinical applications for MSC in stem cell transplantation have been proposed. We have evaluated the frequency, phenotype and differentiation potential of MSC in adult BM, cord blood (CB) and peripheral blood stem cell collections (PBSC). During culture, BM MSC proliferated to confluence in 10-14 d, maintaining a stable non-haemopoietic phenotype, HLA class-1 + , CD29 + , CD44 + , CD90 + , CD45 -, CD34 -and CD14 through subsequent passages. Using the colony forming unit fibroblasts assay, the estimated frequency of MSC in the BM nucleated cell population was 1 in 3AE4 · 10 4 cells. Both adipogenic and osteogenic differentiation of BM MSC was demonstrated. In contrast, CB and PBSC mononuclear cells cultured in MSC conditions for two passages produced a population of adherent, non-confluent fibroblast-like cells with a haemopoietic phenotype, CD45 + , CD14 + , CD34 -, CD44 -, CD90 -and CD29 -. In paired experiments, cultured BM MSC and mature BM stroma were seeded with CB cells enriched for CD34 + . Similar numbers of colony-forming units of granulocytes-macrophages were produced by MSC-based and standard stroma cultures over 10 weeks. We conclude that adult BM is a reliable source of functional cultured MSC, but CB and PBSC are not.

show abstract

“…The aim of this study is to improve the obstetricianbased cord blood collection [7,8] system and an efficient recovery of CD34+ cells ( fig. 1).…”

Section: Resultsmentioning

confidence: 99%

Haematopoietic Progenitors from Umbilical Cord Blood

Todaro

Pafumi²,

Pernicone³

et al. 2000

Blood Purif

View full text Add to dashboard Cite

Background/Aim: The aim of this study is to improve the obstetrician-based cord blood collection system and an efficient recovery of CD34+ haematopoietic progenitor stem cells. Methods: CD34+ cells were purified from total blood using a positive selection enrichment method, called Mini-Macs. Results: The final yield of CD34+ cells we obtained was 10⁴ cells/ml, with a CD34+ purity of 99%. Conclusion: Our results confirm that, by using this method, it is possible to get a significant stem cell number, thus improving transplanting both peripheral stem cells and umbilical cord ones.

show abstract

Different behaviour of fresh and cultured CD34⁺ cells during immunomagnetic separation

Cited by 12 publications

References 17 publications

Human Umbilical Cord Blood CD34-Positive Cells as Predictors of the Incidence and Short-Term Outcome of Neonatal Hypoxic-Ischemic Encephalopathy: A Pilot Study

Human Umbilical Cord Blood CD34-Positive Cells as Predictors of the Incidence and Short-Term Outcome of Neonatal Hypoxic-Ischemic Encephalopathy: A Pilot Study

Adult bone marrow is a rich source of human mesenchymal ‘stem’ cells but umbilical cord and mobilized adult blood are not

Haematopoietic Progenitors from Umbilical Cord Blood

Contact Info

Product

Resources

About

Different behaviour of fresh and cultured CD34+ cells during immunomagnetic separation

Cited by 12 publications

References 17 publications

Human Umbilical Cord Blood CD34-Positive Cells as Predictors of the Incidence and Short-Term Outcome of Neonatal Hypoxic-Ischemic Encephalopathy: A Pilot Study

Human Umbilical Cord Blood CD34-Positive Cells as Predictors of the Incidence and Short-Term Outcome of Neonatal Hypoxic-Ischemic Encephalopathy: A Pilot Study

Adult bone marrow is a rich source of human mesenchymal ‘stem’ cells but umbilical cord and mobilized adult blood are not

Haematopoietic Progenitors from Umbilical Cord Blood

Contact Info

Product

Resources

About

Different behaviour of fresh and cultured CD34⁺ cells during immunomagnetic separation