Different attentional dysfunctions in eEF2K^−/−, IL1RAPL1^−/− and SHANK3Δ11^−/− mice

Abstract

A common feature of several psychiatric disorders is the attentional impairment. eEF2K −/−, IL1RAPL1 −/− and SHANK3Δ11 −/− mice were used as animal models consistently linked to changes in synaptic plasticity, learning and memory. All knockout (KO) mice and their corresponding littermates were submitted to the novel object recognition (NOR) and visual object recognition (VOR) tasks. In the NOR, eEF2K−/− mice exhibited a normal performance in terms of mean discrimination index, while SHANK3Δ11−/− and IL1RAPL1 −/− mice were impaired when a delay of 2 and 24 hours was introduced. Surprisingly, when submitted to VOR, where the two objects were replaced with two shapes delivered from two iPods, all the mutant mice performed worse than those in the NOR. In VOR, the application of motion to different shapes, to increase attention, improved performance in eEF2K −/− and IL1RAPL1 −/− but not in SHANK3Δ11 −/− mice. In SHANK3Δ11 −/− mice, attentional deficit was also present even if different motions were applied to the same shapes or when these mice were repeatedly exposed for 5 days to the context. Behavioral analysis showed that eEF2K−/− and IL1RAPL1 −/− mice had a good flexibility tested in the T‐maze. eEF2K−/− showed normal self‐grooming. On the basis of previous literature data indicating that SHANK3Δ11 −/− showed impaired flexibility and reduced sociability, we identified in this genotype the most exhaustive model showing all the core symptoms of autism spectrum disorder including a heavy visual attention deficit. These findings show the importance of VOR to identify mouse models of autism.

Sex-dependant differences in the ability of nicotine to modulate discrimination learning and cognitive flexibility in mice

“…Mice deficient in Shank, Neuroligin, or PSD-95 complex components have previously been evaluated as mouse models of autism (Qin et al, 2018; Radyushkin et al, 2009; Winkler et al, 2018). SHANK3Δ11 deficiency is known to reduce cognitive flexibility, and Shank3B heterozygous mice demonstrate impaired discrimination learning (Copping et al, 2017; Ponzoni et al, 2019). Among the components of the PSD-95 protein complex, Syngap1 heterozygous mice have been reported to show impaired cognitive flexibility, whereas deletion of neuroligin-3 or Dlgap2 enhanced cognitive flexibility (Horner et al, 2021).…”

“…heterozygous mice demonstrate impaired discrimination learning (Copping et al, 2017;Ponzoni et al, 2019). Among the components of the PSD-95 protein complex, Syngap1 heterozygous mice have been reported to show impaired cognitive flexibility, whereas deletion of neuroligin-3 or Dlgap2 enhanced cognitive flexibility (Horner et al, 2021).…”

IL1RAPL1 Gene Deletion in a Female Patient with Developmental Delay and Continuous Spike-Wave during Sleep

“…16 Deletion of IL1RAPL1 in mice ( IL1RAPL1 −/− mice) produces endophenotypes of ASD including anxiety along with impaired spatial and context-dependent fear memory and behavioral flexibility. 20 Mice with global deletion of PTPδ—a synaptic partner of IL1RAPL1—exhibit impaired non-REM sleep and abnormal sleep behavior. 21 Further clinical investigations and functional studies of IL1RAPL1 in experimental systems may contribute to the function of IL1RAPL1 in brain development as well as to more general mechanisms underlying CSWS and ESES.…”