Different Approaches to Produce Transgenic Virus B Resistant Chrysanthemum

Mitiouchkina, Tatiana; Фирсов, А. А.; Titova, S M; Pushin, Alexander; Shulga, O. A.; Dolgov, Sergey

doi:10.3390/agronomy8030028

Cited by 10 publications

(5 citation statements)

References 24 publications

(12 reference statements)

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“…Overexpression of the coat protein (CP) gene conferred tolerance against CMV [ 151 ]. The sense and double-sense CVB coat protein (CP) gene in transgenic chrysanthemum improved tolerance to chrysanthemum virus B [ 152 ]. Choi et al (2015) have presented a transcriptome analysis of chrysanthemum associated with three RNA viruses including TSWV, CMV, and PVX [ 153 ].…”

Section: Biotic Stress Resistancementioning

confidence: 99%

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

Mekapogu

Kwon

Song

et al. 2022

IJMS

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Incessant development and introduction of novel cultivars with improved floral attributes are vital in the dynamic ornamental industry. Chrysanthemum (Chrysanthemum morifolium) is a highly favored ornamental plant, ranking second globally in the cut flower trade, after rose. Development of new chrysanthemum cultivars with improved and innovative modifications in ornamental attributes, including floral color, shape, plant architecture, flowering time, enhanced shelf life, and biotic and abiotic stress tolerance, is a major goal in chrysanthemum breeding. Despite being an economically important ornamental plant, the application of conventional and molecular breeding approaches to various key traits of chrysanthemum is hindered owing to its genomic complexity, heterozygosity, and limited gene pool availability. Although classical breeding of chrysanthemum has resulted in the development of several hundreds of cultivars with various morphological variations, the genetic and transcriptional control of various important ornamental traits remains unclear. The coveted blue colored flowers of chrysanthemums cannot be achieved through conventional breeding and mutation breeding due to technical limitations. However, blue-hued flower has been developed by genetic engineering, and transgenic molecular breeding has been successfully employed, leading to substantial progress in improving various traits. The recent availability of whole-genome sequences of chrysanthemum offers a platform to extensively employ MAS to identify a large number of markers for QTL mapping, and GWAS to dissect the genetic control of complex traits. The combination of NGS, multi-omic platforms, and genome editing technologies has provided a tremendous scope to decipher the molecular and regulatory mechanisms. However, the application and integration of these technologies remain inadequate for chrysanthemum. This review, therefore, details the significance of floral attributes, describes the efforts of recent advancements, and highlights the possibilities for future application towards the improvement of crucial ornamental traits in the globally popular chrysanthemum plant.

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Section: Biotic Stress Resistancementioning

confidence: 99%

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

Mekapogu

Kwon

Song

et al. 2022

IJMS

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“…The chrysanthemum plants (Chrysanthemum morifolium Ramat) of the cultivar White Snowdon were used in all experiments. Agrobacterium-mediated transformation and in vitro culture of chrysanthemum plants were performed as described previously [31,32].…”

Section: Agrobacterial Transformation Of Chrysanthemummentioning

confidence: 99%

Agrobacterium-Mediated Transformation of Chrysanthemum with Artemisinin Biosynthesis Pathway Genes

Фирсов

Mitiouchkina

Shaloiko

et al. 2020

Plants

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Artemisinin-based drugs are the most effective medicine for the malaria treatment. To date, the main method of artemisinin production is its extraction from wormwood plants Artemisia annua L. Due to the limitation of this source, considerable efforts are now directed to the development of methods for artemisinin production using heterologous expression systems. Artemisinin is a sesquiterpene lactone, synthesized through the cyclization of farnesyl diphosphate involved in other sesquiterpene biosynthetic systems. Chrysanthemum species as well as A. annua, belong to Asteraceae family, and had been characterized by containing highly content of sesquiterpenes and their precursors. This makes chrysanthemum a promising target for the production of artemisinin in heterologous host plants. Chrysanthemum (C. morifolium Ramat.) was transformed by Agrobacterium tumefaciens carrying with the binary vectors p1240 and p1250, bearing artemisinin biosynthesis genes coding: amorpha-4,11-diene synthase, artemisinic aldehyde Δ11(13) reductase, amorpha-4,11-diene monooxygenase (p1240 was targeted to the mitochondria and p1250 was targeted to the cytosol), cytochrome P450 reductase from A. annua, as well as yeast truncated 3-hydroxy-3-methylglutarylcoenzyme A reductase. This study obtained 8 kanamycin-resistant lines after transformation with the p1240 and 2 lines from p1250. All target genes were detected in 2 and 1 transgenic lines of the 2 vectors. The transformation frequency of all target genes were 0.33% and 0.17% for p1240 and p1250, relative to the total transformed explant numbers. RT-PCR analysis revealed the transcription of all transferred genes in two lines obtained after transformation with the p1240 vector, confirming the possibility of transferring genetic modules encoding entire biochemical pathways into the chrysanthemum genome. This holds promise for the development of a chrysanthemum-based expression system to produce non-protein substances, such as artemisinin.

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“…With the improvement of living standards, people’s desire for better health is increasingly urgent, and the market demand for functional chrysanthemums is also growing daily. However, with the continuous expansion of the cultivation area for functional chrysanthemums, viral infection is common in the growth process [ 24 , 25 , 26 , 27 , 28 , 29 ], and chrysanthemums can be infected by a variety of viruses at the same time [ 30 ]. In addition, chrysanthemum is heterohexaploid [ 31 , 32 ], with a complex genetic background and high variability in hybrid offspring traits.…”

Section: Introductionmentioning

confidence: 99%

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

Gao,

Chen,

Pan

et al. 2023

Viruses

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Chrysanthemum is an important functional plant that is used for food, medicine and tea. Functional chrysanthemums become infected with viruses all around the world, seriously lowering their quality and yield. Viral infection has become an important limiting factor in chrysanthemum production. Functional chrysanthemum is often propagated asexually by cutting during production, and viral infection of seedlings is becoming increasingly serious. Chrysanthemums can be infected by a variety of viruses causing different symptoms. With the development of biotechnology, virus detection and virus-free technologies for chrysanthemum seedlings are becoming increasingly effective. In this study, the common virus species, virus detection methods and virus-free technology of chrysanthemum infection are reviewed to provide a theoretical basis for virus prevention, treatment and elimination in functional chrysanthemum.

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Different Approaches to Produce Transgenic Virus B Resistant Chrysanthemum

Cited by 10 publications

References 24 publications

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

Agrobacterium-Mediated Transformation of Chrysanthemum with Artemisinin Biosynthesis Pathway Genes

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

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