Different Apoptotic Effects of Triterpenoid Saponin-Rich <i>Gypsophila oldhamiana</i> Root Extract on Human Hepatoma SMMC-7721 and Normal Human Hepatic L02 Cells

Abstract

The roots of Gypsophila oldhamiana are rich in triterpenoid saponins with antitumor properties. Although previous reports have revealed the anticancer potency of some Gypsophila species, the underlying molecular mechanisms of this activity have not been studied in detail. The purpose of the present study was to prepare a triterpenoid saponin-rich G. oldhamiana root extract (TGOE) determined by LC-electrospray ionization (ESI)-MS n for biological studies and to evaluate the different anti-proliferative activities and apoptotic effects of TGOE on human hepatoma SMMC-7721 and normal human hepatic L02 cells.

Cytotoxic Effect of Bitter Melon (Momordica charantia L.) Ethanol Extract and Its Fractions on Pancreatic Cancer Cells in vitro

“…29, 30 Similarly, saponin-rich extracts selectively induced apoptosis in hepatoma cells, without causing the same effect in their normal counterparts. 31 In this study, the SE did not discriminate in its cytotoxic activity and, in fact, was more potent to the HPDE cells. The lower doses (125 and 250 µg/mL) required longer treatment times, of 72 or 96 h, before any observed Richmond R.A. et al: Cytotoxicity of bitter melon for pancreatic cancer Explor Res Hypothesis Med reduction in cancer cell viability, specifically for the CFPAC-1 and BxPC3 cells, which showed no response to SE in the first 24 h. This may be due to differences between the mechanisms and kinetics of cell death, such as the cells' ability to absorb the compounds in a given time.…”

Selective Profiling of Saponins from Gypsophila trichotoma Wend. by HILIC Separation and HRMS Detection

“…Except for the fully characterised saponin references from G. trichotoma , the tentative identification of the GTR saponins was based on HRMS‐measurements, comparison with mass fragmentation observed for the GOTCAB references and literature data (Chen et al ., , Voutquenne‐Nazabadioko et al ., ; Zhang et al ., ).…”

“…HILIC is an orthogonal chromatographic version of the reversed phase mode and two‐dimensional chromatography combining both of them has been applied for analytical (Xing et al ., ) and preparative (Guo et al ., ) separations of saponins. In addition, modern hyphenated techniques combining better resolved and faster chromatography with mass spectrometric detection (MS, MS/MS and HRMS) allow fairly rapid dereplication, or de novo‐ identification, albeit tentative pending more rigorous structural elucidation studies of saponins (Xing et al ., ; Zhang et al ., ; Huang et al, ).…”

Suppression of Hepatitis B Virus Production and Inflammatory Response in vitro and in vivo by Mormodica charantia Compound EMCDO

“…The triterpenoid saponin-rich G. oldhamiana root extract (TGOE) selectively inhibited the proliferation of hepatoma SMMC-7721 cells in a dose-dependent manner, while the cytotoxic effects of TGOE on normal hepatocyte L02 cells were much lower. TGOE preferentially induced apoptosis in SMMC-7721 cells due to the regulation of caspase-3 and mitogen-activated protein kinases (MAPKs) (Zhang, Luo, Zhang, & Kong, 2013). In conclusion, the chemical structure determination of 8 isolated Mormodica charantia compounds.…”