Different alterations in lactate dehydrogenase activity and profile of peripheral blood mononuclear cells in Hodgkin's and non‐Hodgkin's lymphomas

Abstract

The enzyme lactate dehydrogenase (LDH) activity of peripheral blood mononuclear cells (PBMC), LDH isotype H and M pattern and PBMC spontaneous LDH release activity were examined in 55 non-Hodgkin's lymphoma (NHL) patients, 46 Hodgkin's disease (HD) patients and 47 controls. The intracellular LDH and M isotype activity of PBMC, their spontaneous LDH release activity significantly increase (p < 0.01) in NHL with progressing histological grade of malignancy. Contrary to this, all classical HD patients have a significant elevation (p < 0.05) of each of these parameters. Furthermore, unlike HD, in NHL clinical stage is associated with significant (p < 0.05) increase in the level of spontaneous LDH release activity in each histological form. It is also shown that spontaneous LDH release activity of PBMC for HD and NHL patients demonstrates significant positive correlation (p < 0.005) with serum LDH level, although elevation of spontaneous LDH release precedes serum LDH increase in both diseases. The results obtained regarding alterations in intracellular, isotype and spontaneously released LDH activity of circulating PBMC show that these parameters are dependent, in NHL patients, on the grade of malignancy and tumor burden, while they are persistently present in HD patients.

Estimation of cell membrane alteration after drug treatment by LDH release

“…3,4 None contained sufficient dysplasia to be classified as acute leukemia with multilineage dysplasia, a diagnosis that requires dysplasia in at least 50% of the cells of at least 2 lineages. 2 In addition, the one case with more than 80% erythroid precursors revealed erythroid maturation and did not meet the criteria for pure erythroid leukemia. 2 All 5 cases were diagnosed as acute erythroleukemia.…”

“…2 In addition, the one case with more than 80% erythroid precursors revealed erythroid maturation and did not meet the criteria for pure erythroid leukemia. 2 All 5 cases were diagnosed as acute erythroleukemia.…”

“…2 Microassay for estimation of cell death process in vitro, by LDH release, was recommended. The assay is based on substrate mixture, using the small volume of culture cells or their supernates.…”

Metabolic regulation of NK cells in blood cancers: Lipid-rich environment in blood cancers is associated with metabolic and functional changes of NK cells

“…Increased lactate levels increase the acidity of the environment, which has been demonstrated to affect the cytotoxic potential of NK cells (Brand et al, 2016;Potzl et al, 2017). In accordance, elevated serum levels of lactate dehydrogenase (LDH), a metabolic enzyme which facilitates interconversions between lactate and pyruvate, are observed in advanced lymphoma, and are strongly correlated with NK cell dysfunction (Jurisic et al, 2000;Konjevic et al, 1999). Similarly, adenosine, which is derived from increased breakdown of extracellular ATP by tumours, also negatively affects NK cell function (Hoskin et al, 2008;Raskovalova et al, 2006;Williams et al, 1997).…”

“…Indeed, NK cells become dysfunctional in lymphomas, and the degree of dysfunction is associated with lymphoma progression and aggressiveness (Danielou-Lazareth et al, 2013;Konjevic et al, 1999;Reiners et al, 2013). In lymphoma patients, elevated serum levels of lactate dehydrogenase (LDH), a metabolic enzyme which promotes interconversion between lactate and pyruvate, is associated with suppressed NK cell function in B cell lymphoma (Jurisic et al, 2000;Konjevic et al, 2012;Rai and Bhatnagar, 2016).…”