Different allele frequencies of MC4R gene variants in Chinese pig Breeds

Chen, Min; Wang, Aiguo; Fu, Jinlian; Li, Ning

doi:10.5194/aab-47-463-2004

Cited by 8 publications

(3 citation statements)

References 14 publications

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“…Study about melanocortin gen also have done before in Magelang ducks and Ettawa goats (Rahayu, et al, 2015;Maharani et al, 2016). The melanocortinergic system has been detected in association with body weight in the agouti mouse (Chen et al, 2004). MC4R gene could activate leptin signaling for food intake and body weight in Mice (Marsh et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

Maharani

Fathoni

Sumadi

et al. 2018

J. Indonesian Trop. Anim. Agric.

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ABSTRAKGen MC4R dikenal sebagai kandidat gen untuk sifat pertumbuhan. Penelitian ini bertujuan untuk mengidentifikasi gen MC4R pada sapi Peranakan Ongole (PO) Kebumen dan hubungannya terhadap sifat pertumbuhan ternak tersebut. Data bobot lahir (BL), bobot sapih (BS), panjang badan lahir (PBL), lingkar dada lahir (LDL), tinggi gumba (TG), panjang badan sapih (PBS), lingkar dada sapih (LDS), tinggi gumba sapih (TGS) dan rata-rata pertumbuhan bobot badan harian (ADG) digunakan untuk analisis asosiasi berdasarkan gen MC4R. Total 60 sampel darah diambil dan digunakan untuk isolasi DNA.Produk PCR diamplifikasi dengan menggunakan primer forward 5'-GTCGGGCGTCTTGTTCATC-3' dan reverse 5' GCTTGTGTTTAGCATCGCGT-3'. Single Nucleotide Polymorphisms (SNP) g.1133 C>G digunakan untuk proses genotyping menggunakan metode PCR-RFLP dengan enzim restriksi HpyCH4IV. Frekuensi alel G (0,59) lebih besar dibandingkan dengan alel C (0.41). Frekuensi genotip tertinggi dimiliki oleh ternak bergenotip heterozigot CG, diikuti dengan ternak bergenotipe homozigot GG (0,33) dan CC (0,15). Hasil uji Chi-square menunjukkan populasi tidak menyimpang (P>0,05) dari Hardy-Weinberg Equilibrium (HWE). Hasil analisis asosiasi menunjukkan bahwa SNP g. 1133 C>G pada gen MC4R berpengaruh nyata terhadap panjang badan lahir ternak dengan genotip GG (P<0,05). Kesimpulannya, SNP g.1133 C>G dapat digunakan sebagai marker untuk seleksi sapi PO Kebumen berdasarkan sifat panjang badan saat lahir.Kata kunci: sapi PO Kebumen, Gen MC4R, bobot badan, ukuran tubuh ABSTRACT MC4R gene is known as an important candidate gene for the growth trait. The purpose of this research was to identify the MC4R gene in Kebumen Ongole grade cattle and examine its association with growth traits. Data of birth weight (BW), weaning weight (WW), birth body length (BBL), birth chest circumference (BCC), birth shoulder height (BSH), weaning body length (WBL), weaning chest circumference (WCC), weaning shoulder height (WSH) and average daily gain (ADG) were collected and used for analysis of MC4R gene. Sixty blood samples were collected for DNA isolation and PCR amplification. The single nucleotide polymorphisms (SNP) g.1133 C>G was used for genotyping by using PCR-RFLP methods. The frequenciy of G allele (0.59) was greater than C allele (0.41). The highest genotype frequencies have been detected in CG heterozygote animals (0.52) followed by GG (0.33) and CC (0.15) in homozygote animals. The results of Pearson 's Chi-square test indicated that the population was not deviate (P>0.05) from the Hardy-Weinberg equilibrium (HWE). The SNP g. 1133 C>G of MC4R gene indicated affecting high birth body length with GG genotype (P<0.05). In Kebumen Ongole Grade Cattle (D. Maharani et al.) 87 conclusion, the SNP g. 1133 C>G may can be a marker for birth body length of calf selection. Identification of MC4R Gene in

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Section: Introductionmentioning

confidence: 99%

Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

Maharani

Fathoni

Sumadi

et al. 2018

J. Indonesian Trop. Anim. Agric.

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“…It has been established that an essential factor in increasing the production of high-quality pork is the intensification of the breeding process in the field of pig breeding (Chen et al, 2004;Tsereniuk et al, 2010;Biriukova & Makovska, 2011;Khramkova, 2019;Dudka, 2020;Xu et al, 2021;Li et al, 2021). It provides for the introduction of innovative methods of evaluation and selection of highly productive animals, the use of animals of foreign breeding, as well as the study of the level of their operational value in the conditions of industrial technology of keeping (Kim et al, 2000;Fontanesi et al, 2010;Rodríguez et al, 2013;Herrero-Medrano et al, 2015;Tsybenko et al, 2021;Vashchenko & Berezovskyi, 2021;Khalak et al, , 2022Khalak et al, , 2023.…”

Section: Introductionmentioning

confidence: 99%

Reproductive qualities of French breed large white breed sows of different operating value and level of their discretion

Zasukha,

Voloshchuk,

Khalak

et al. 2024

Ukr. Jour. of Vet. and Agr. Sci.

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This work aimed to investigate the lifespan and breeding use, reproductive qualities, and the level of their discreteness in sows of the large white breed of French breeding, considering their operational value. Agroprime Holding LLC of the Odesa region, the laboratory of innovative technologies, and experimental livestock facilities of the Institute of Pig Breeding and AIP of the National Academy of Sciences carried out the practical part of the work. Evaluation of sows according to indicators characterizing the level of their adaptation and reproductive qualities was carried out taking into account the following characteristics: life expectancy, days; duration of tribal use, days; received farts; all live piglets were born, head.; multifertility, ch.; high fertility, kg.; nest weight at the time of weaning at 28 days, kg; preservation, %. The selection index of reproductive qualities of the sow (SIRQS) was calculated according to the method of O. M. Tsereniuk et al. (2010), the coefficient of discreteness (D) – Seromolot and Sviatchenko (1984). Biometric processing of research results was carried out according to generally accepted methods. It has been established that sows of the large white breed of French breeding, provided they are kept in production premises using innovative technologies, are characterized by high indicators of the level of adaptation and reproductive qualities. Thus, their life span is 905.7 days, the duration of breeding use is 680.6 days, and in terms of reproductive qualities, they belong to the elite class. A significant difference between the groups of experimental animals was established in terms of life expectancy, duration of breeding use, total number of live piglets, multifertility, and litter weight at the time of weaning at the age of 28 days. The maximum indicators of high fertility (1.34 kg) and preservation of piglets until weaning at the age of 28 days (100 %) were found in sows of the “low operational value” category. The coefficient of discreteness of life expectancy and breeding use in sows of I, II, and III experimental groups is equal to 0.406, 0.677, and 0.633, reproductive qualities – 0.828, 0.882, and 0.903, respectively. Coefficients of paired correlation between life span and breeding use of sows and signs of reproductive qualities range from -0.055 to +0.904. The maximum increase in additional production was obtained from sows of the “high operational value” category – +6.80 %, and its value is +355.57 UAH/head/ farrowing.

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“…Intensification of the breeding process, as well as the introduction of modern technologies for keeping and feeding pigs of different sex and age groups, determine the work program of agricultural formations, the goal of which is to create highly profitable pork production (Voloshchuk & Vasyliv, 2013;Bankovska, 2016;Barkar & Dekhtiar, 2017;Voloshchuk & Hryshyna, 2017;Martyshuk et al, 2021). At the same time, the essential issues are the objective assessment of the level of animal productivity and the search for effective methods of early prediction of the reproductive qualities of sows and breeding boars, as well as the fattening and meat qualities of their offspring Chen et al, 2004;Fontanesi et al, 2010;Zorc et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Activity of blood serum enzymes and their relationship with feeding and meat qualities in young pigs of different intrageneric differentiation according to the “formation intensity” index

Khalak¹,

Gutyj

2023

Ukr. Jour. of Vet. and Agr. Sci.

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The article presents the study results of the activity of blood serum enzymes, fattening, and meat qualities of young pigs of the universal direction of productivity, considering their intrabreed differentiation according to the “formation intensity” index. It was established that the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in the blood serum of young pigs of the large white breed corresponds to the physiological norm of clinically healthy animals; by the age of reaching a live weight of 100 kg, the thickness of lard at the level of 6–7 thoracic vertebrae and the length of the chilled carcass – I class and elite class. Taking into account the intrabreed differentiation of young pigs of the large white breed according to the “formation intensity” index, the animals of the experimental group I (∆ = 0.715–1.009) exceeded those of the same age as II (∆ = 1.011–1.356) in terms of average daily increase in live weight by 5.71%, age reaching a live weight of 100 kg – 1.28 %, fat thickness at the level of 6–7 thoracic vertebrae – 1.44 %. The pairwise correlation coefficient between biochemical indicators of blood serum and fattening and meat qualities of young pigs of the large white breed shows that this biometric indicator varies from -0.344 ± 0.1485 to +0.402 ± 0.1448. The maximum increase in additional production was obtained from young pigs of the first experimental group, in which the “formation intensity” index ranges from 0.715 to 1.009 points (+3.40 %). The criterion for selecting highly productive animals of the leading herd based on the absolute parameters of their offspring's fattening and meat qualities is their correspondence to the elite class, according to the “intensity of formation” index – 0.715–1.009 points

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Different allele frequencies of MC4R gene variants in Chinese pig Breeds

Cited by 8 publications

References 14 publications

Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

Reproductive qualities of French breed large white breed sows of different operating value and level of their discretion

Activity of blood serum enzymes and their relationship with feeding and meat qualities in young pigs of different intrageneric differentiation according to the “formation intensity” index

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