Different 4T1 Cells Migration under Caesalpinia sappan L. and Ficus septica Burm.f Ethanolic Extracts

Haryanti, Sari; Murwanti, Retno; Putri, Herwandhani; Ilmawati, Gagas Pradani Nur; Pramono, Suwijiyo; Meiyanto, Edy

doi:10.14499/indonesianjcanchemoprev8iss1pp21-26

Cited by 9 publications

(13 citation statements)

References 13 publications

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“…EEDAA juga terbukti menginduksi apoptosis dan menurunkan ekspresi protein antiapoptosis Bcl-2 pada sel MCF-7 (Sekti et al, 2010). Selain itu, EEDAA bersifat sitotoksik kuat pada sel 4T1 (Haryanti et al, 2017), suatu tipe sel yang memiliki kemampuan metastasis (Heppner et al, 2000). Sedangkan, hasil studi in vivo dengan J Pharm Sci Clin Res, 2021, 03 301 dosis 750 mg/kgBB meningkatkan apoptosis pada tikus yang diinduksi DMBA sebagai model kanker payudara melalui p53-independent pathway (Anindyajati et al, 2012).…”

Section: Sel Kanker Ekstrakunclassified

“…Begitu juga turunan alkaloid fenantroindoisidin lainnya, antofin, yang menghambat migrasi dengan pembentukan tube sel endotel serta menghambat proses signaling ke reseptor (Nurhasanah et al, 2018). Ekstrak etanolik daun awar-awar sendiri juga terbukti mampu menghambat metastasis dengan menurunkan aktivitas dan ekspresi MMP-9 dan Rac-1 (Haryanti et al, 2017). Rac-1 merupakan protein yang mengatur pertumbuhan dan motilitas sel kanker melalui pembentukan lamellipodia (Parri & Chiarugi, 2010).…”

Section: Potensi Daun Awar-awar Sebagai Antimetastasisunclassified

“…F.) mengandung senyawa potensial golongan alkaloid fenantroindolisidin (Damu et al, 2005). Ekstrak dan alkaloid fenantroindolisidin yang dikandungnya terbukti memiliki efek sitotoksik dan menghambat metastasis kanker payudara (Haryanti et al, 2017;Mandhare, 2015). Ketika dikombinasikan dengan agen kemoterapi, daun awar-awar menunjukkan efek sinergis dalam meningkatkan sitotoksisitas sel kanker (Nugroho et al, 2013).…”

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Review Potensi Daun Awar-awar Sebagai Agen Ko-Kemoterapi Antimetastasis Kanker Payudara

Salsabila

Faqiha

Anggraeni

et al. 2021

J. Pharm. Sci. Clin. Res.

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<p>Kematian akibat kanker payudara sebagian besar disebabkan oleh metastasis. Kemoterapi sebagai agen terapi kanker payudara juga menimbulkan efek samping metastasis. Daun awar-awar (<em>Ficus septica</em> Burm. F.) mengandung senyawa golongan alkaloid fenantroindolisidin. Ekstrak daun awar-awar dan alkaloid fenantroindolisidinnya berefek sitotoksik pada sel kanker payudara dan sinergis terhadap agen kemoterapi. Artikel ini mengulas potensi daun awar-awar sebagai agen pendamping kemoterapi (ko-kemoterapi), khususnya melalui mekanisme antimetastasis. Aktivitas sitotoksik daun awar-awar pada sel kanker terjadi melalui peningkatan apoptosis dan modulasi siklus sel. Daun awar-awar menghambat metastasis sel kanker payudara dengan menurunkan ekspresi VEGF, MMP-9, dan Rac-1 yang terlibat dalam metastasis. Oleh karena itu, dapat disimpulkan bahwa daun awar-awar berpotensi untuk dikembangkan sebagai agen ko-kemoterapi antimetastasis untuk kanker payudara. </p>

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Section: Sel Kanker Ekstrakunclassified

Section: Potensi Daun Awar-awar Sebagai Antimetastasisunclassified

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Review Potensi Daun Awar-awar Sebagai Agen Ko-Kemoterapi Antimetastasis Kanker Payudara

Salsabila

Faqiha

Anggraeni

et al. 2021

J. Pharm. Sci. Clin. Res.

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“…Namun demikian, aktivitas antimetastasis secang tergolong lemah dibandingkan daun awar-awar (Ficus septica). 5 Secang menghambat siklus sel fase G2/M pada sel MCF-7 dan MCF-7/HER2. 6,7 Lempuyang atau Zingiber zerumbet L. Roscoe ex Sm.…”

Section: Pendahuluanunclassified

Efek Sinergis Kombinasi Ekstrak Etanolik Kayu Secang dan Rimpang Lempuyang pada Sel Kanker Payudara MCF-7

Haryanti¹,

Sholikhah²,

Widiyastuti³

2019

jki

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Cancer is one of critical, chronic, and complex disease, also becoming the high cause of death in the world. Brazilin and brazilein in sappan wood (Caesalpinia sappan L.) and zerumbone in bitter ginger rhizome (Zingiber zerumbet L.) are known having cytotoxic activity with different mechanisms. This study aimed to examine combination effect of sappan wood and bitter ginger rhizome. Sappan wood and bitter ginger rhizomes macerated with ethanol 96% for 3x24 hours, filtered, and evaporated to obtain dried extract.Cytotoxic effect on MCF-7 cells was done using MTT assay. Combination Index (CI) was determined by CompuSyn based on the result of cytotoxic combination. Cell cycle profile and apoptosis induction was analyzed by flow cytometry. Sappan wood extracts and bitter ginger rhizome exhibited cytotoxic effects on MCF-7 cells with the IC50 values of 30 and 155 μg/mL respectively. The combination of sappan wood 15 μg/mL and bitter ginger 8, 12, 24, and 60 μg/mL produced synergistic effect with the CI value of 0.57-0.85. Sappan wood 15 μg/mL combined with bitter ginger 8 and 24 μg/mL showed cell cycle inhibition at G2/M phase. The combination also increased apoptosis induction compared to untreated cells and its single treatment.The combination of sappan wood ethanolic extracts and bitter ginger rhizome showed synergistic cytotoxic effect. Its synergism effect revealed through cell cycle arrested at G2/M phase and acceleration of apoptotic induction.

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“…Sappan wood (Caesalpinia sappan L.) is one of the plants containing potential compounds as chemoprevention that promise cytotoxic and anti-MMP activities in various highly metastatic cancer cell lines such as MDA-MB-231, 4T1, MCF-7/HER2, and T47D (Hsieh, et al, 2013;Handayani, et al, 2016;Haryanti, et al, 2017;Jenie, et al, 2020;Handayani, et al, 2020). The mechanisms, including down-regulates the expression of MMP9, MMP2, Rac1, and p120; inhibits the gelatin-degradation activity of MMP9 and MMP2, and inhibits cell migration and invasion.…”

Section: Introductionmentioning

confidence: 99%

Revealing the Potential of Compounds in Sappan Wood as Cervical Cancer Metastasis Chemopreventive Agent With MMP9 Target

Hastuti

Adisusilo

Ashar

et al. 2022

Indones J Cancer Chemoprevent

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Matrix metalloproteinase-9 (MMP9) has an essential role in cervical cancer metastasis. Sappan wood extract (SWE) from Caesalpinia sappan contains metabolites that have pharmacological effects and can potentially inhibit metastasis by targeting the protein markers. This research aims to discover the potency of compounds in C. sappan as chemopreventive agents for metastasis in cervical cancer by targeting MMP9. SWE was obtained by maceration with methanol and analyzed using thin layer chromatography (TLC). In vitro cytotoxicity test of SWE on HeLa cells was performed by direct counting method. MMP9 expression profiles and survival rates in cervical cancer patients were explored through bioinformatics studies by the GEPIA database. The CMAUP and PubChem databases were used to obtain the metabolomic profile of SWE. SWE compounds’ activities on target proteins were obtained through KNIME software, while its interaction with MMP9 was analyzed using molecular docking with MOE software. We obtained SWE with a yield of 9.7% w/w. The extract contains brazilin and is indicated by the spot appearance at Rf 0.375. The cytotoxicity of SWE against HeLa cells was considered potential as the IC50 value was 54.93 μg/mL. Based on the bioinformatics analysis, there is a significant difference in MMP9 expression between normal and cervical cancer tissue. The patient’s survival probability decreased if MMP9 was overexpressed. The molecular docking results showed that active compounds of SWE bind to the MMP9 inhibition site with higher affinity compared to the native ligand. This study reveals that SWE potential to be developed as a chemopreventive agent through metastasis inhibition in cervical cancer by targeting MMP9.Keywords: Caesalpinia sappan L., metastasis, bioinformatics, molecular docking, MOE.

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Different 4T1 Cells Migration under Caesalpinia sappan L. and Ficus septica Burm.f Ethanolic Extracts

Cited by 9 publications

References 13 publications

Review Potensi Daun Awar-awar Sebagai Agen Ko-Kemoterapi Antimetastasis Kanker Payudara

Review Potensi Daun Awar-awar Sebagai Agen Ko-Kemoterapi Antimetastasis Kanker Payudara

Efek Sinergis Kombinasi Ekstrak Etanolik Kayu Secang dan Rimpang Lempuyang pada Sel Kanker Payudara MCF-7

Revealing the Potential of Compounds in Sappan Wood as Cervical Cancer Metastasis Chemopreventive Agent With MMP9 Target

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