Differencial proteome of clear-cell renal cell carcinoma (ccRCC) tissues

Abstract

Purpose: We attempted to detect, for the first time in a Brazilian cohort, differences in protein expression between clear-cell renal cell carcinoma (ccRCC) and their normal adjacent tissues, aiming to identify biomarkers and/or therapeutic target candidates for this disease. Material and Methods: Twenty-four ccRCC and adjacent normal tissues were collected after surgery and their protein extracts were quantified, pooled and separated by two--dimensional polyacrylamide gel electrophoresis (2DE), followed by statistical analysis of the stained gels. Spots of interest were excised from the gels, digested with trypsin and identified by MALDI-TOF-TOF mass spectrometry. Results: Twenty-six differential spots were detected between the two classes of tissues, among which twenty were identified by mass spectrometry and sixteen were found to be non-redundant. Eleven proteins were either underexpressed or undetected in the ccRCC extracts, such as prohibitin and peroxiredoxin-3, whereas five were found to be overexpressed or exclusively detected in the ccRCC extract, including αβ crystalin and heat shock protein 27. Conclusions: Several proteins were detected at differential levels when compared to normal adjacent tissues, and, moreover, many have been previously described by their relationship with RCC. Therefore, this work corroborates previous reports on the search for biomarkers for ccRCC, as well as it points out new candidates that may be validated in future studies.

CT diagnosis for metastasis of clear cell renal cell carcinoma to the pancreas

“…Tumor markers such as CEA, CA125, and CA19-9 may rise slightly in few cases, but there is no specificity. [13] Among 3 cases in our study, only one case (Case 2) showed slightly increased CEA, while the rest tumor markers were all normal in all 3 cases. Therefore, quite a lot of pancreatic metastases from CCRCC are detected during check-ups or routine physical examination.…”

Differential Diagnosis of Metastatic Lesions in the Pancreas in Patients with Renal Cell Carcinoma and Focal Chronic Pancreatitis: A Clinical Observation

“…В некоторых случаях заболевание протекает бессимптомно. Онкомаркеры, такие как раково-эмбриональный антиген и СА19-9, могут незначительно повышаться, но специфичность отсутствует [15]. В обзоре 236 случаев метастазов ПКР как одиночных, так и множественных, F. Sellner и соавт.…”

Proteomic approaches for characterizing renal cell carcinoma

“…The individual spots visualized by this methodology are then excised and subjected to mass spectrometry analysis to identify and annotate the protein spots, with differences in protein spot intensity between experimental conditions inferring differential protein abundance and identification of dysregulated proteins. Multiple studies have leveraged this technique to identify differential expressed protein profiles between ccRCC and NAT samples, followed by immunoblotting or immunohistochemical validation in independent samples [ 26 – 30 ]. Perroud et al [ 26 ], identified 31 proteins that were differentially expressed between ccRCC tumor tissues and NATs derived from four patients, validating the overexpression of HSP27 (HSPB1) and PKM2 via immunoblotting.…”