Differences in Virulence Between Bovine-Derived Clinical Isolates of Pasteurella multocida Serotype A from the UK and the USA in a Model of Bovine Pneumonic Pasteurellosis

Dagleish, Mark P.; Bayne, C.W.; Moon, G.G.; Finlayson, J.; Sales, Jill; Williams, James T.; Hodgson, John

doi:10.1016/j.jcpa.2016.05.010

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…Pasteurella multocida is a gram-negative pathogen and has various host types, including cattle, birds, pigs, cats, and dogs ( 31 ). Among them, bovine PmA is one of the most prevalent pathogen in cattle around the world, causing representative respiratory tract syndrome with high lethality ( 32 , 33 ). In China, since 2008, the respiratory pathogen bovine PmA has replaced P. multocida serogroup B to become the most prevalent pathogen in clinics.…”

Section: Discussionmentioning

confidence: 99%

L-Ascorbic Acid Shapes Bovine Pasteurella multocida Serogroup A Infection

Zhao

et al. 2021

Front. Vet. Sci.

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Bovine Pasteurella multocida serogroup A (bovine PmA) is one of the most important pathogens causing fatal pneumonia in cattle. However, it is largely unknown how nutrition shapes bovine PmA infection. Here, we discovered that the infected lung held the highest bacterial density than other tissues during infection. By screening the different metabolites between high (lung)- and low (liver)-bacterial density tissues, the present work revealed that L-ascorbic acid and L-aspartic acid directly influenced bovine P. multocida growth. Interestingly, L-ascorbic acid, which is expressed at higher levels in the infected livers, inhibited bovine PmA growth as well as virulence factor expression and promoted macrophage bactericidal activity in vitro. In addition, ascorbic acid synthesis was repressed upon bovine PmA infection, and supplementation with exogenous L-ascorbic acid significantly reduced the bacterial burden of the infected lungs and mouse mortality. Collectively, our study has profiled the metabolite difference of the murine lung and liver during bovine PmA infection. The screened L-ascorbic acid showed repression of bovine PmA growth and virulence expression in vitro and supplementation could significantly increase the survival rate of mice and reduce the bacterial load in vivo, which implied that L-ascorbic acid could serve as a potential protective agent for bovine PmA infection in clinic.

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Section: Discussionmentioning

confidence: 99%

L-Ascorbic Acid Shapes Bovine Pasteurella multocida Serogroup A Infection

Zhao

et al. 2021

Front. Vet. Sci.

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“…In contrast, the colonisation and proliferation of bacterial pathogens in bovine lungs are closely related to bovine pneumonia (Confer, 2009), and the three target bacteria can invade lymph nodes (Nattermann and Horsch, 1977;Shoo et al, 1990;Dagleish et al, 2016). Therefore, combined with the necropsy results, the detection results obtained from tissue samples could be more precise than those from bovine upper respiratory tract samples in determining whether or not these bacteria are involved in the disease.…”

Section: Discussionmentioning

confidence: 99%

Development of a multiplex PCR for simultaneous detection of Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes

Zhang

Liu

et al. 2017

Acta Veterinaria Hungarica

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Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes are three bacterial pathogens closely associated with the bovine respiratory disease complex (BRDC). In the current study, a multiplex PCR for the simultaneous detection of these three bacteria in cultures was established. After serial optimisation, the detection limit of the method for the genomic DNA of the three bacteria was 40 pg/μl. The method could detect the genomic DNA of these three bacteria but not the genomic DNA of seven other bacterial strains. Together with the bacterial enrichment technology, the multiplex PCR could be used for detecting the three bacteria in animal tissues. This method might be valuable for speeding up laboratory diagnosis and directing the treatment of BRDC to these three bacterial pathogens.

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“…Challenge organisms were calculated by plate count method to determine colony forming units (CFUs) / ml. The authentic mean dose was determined, post inoculation, looking back from viable/plate counts as is described by [9].…”

Section: Fresh Culturesmentioning

confidence: 99%

Pathological Study On Experimental Infection With Mycoplasma Mycoides Subspecies Capri In Different Age Groups of Goats

Ayaz Hussain Panhwar,

Mansoor Tariq,

Amjad Ali Channa

et al. 2023

biosight

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Introduction: To determine clinico-pathology caused by Mycoplasma mycoides subspecies capri (PG3) in goats of different age groups and antibody titers. Methods: Twenty-one goats of three age groups viz; 1, 2 and 3 years with 7 goats of each age. Goats were divided in four groups i-e; G1, G2, G3 each contain 5 goats of each age while G4 comprise 6 goats; 2 from each group. Goats in G1, G2 and G3 were infected with Mycoplasma mycoides (PG3) dose (1×107 CFU/ml/kg) whereas, G4 was kept as control. Clinical examinations were recorded at 12-h intervals. The blood samples collected were tested through cELISA and the specimen of trachea, lungs, kidney, and liver were collected at the end of experiment for gross and histopathology. Results: Temperature, respiratory rate, pulse rate nasal discharge, coughing and lacrimation were noted higher in G1 compared to G2 and G3 groups. Gross pathology showed severe multifocal and diffused necrosis G1 compared to G2 and G3 groups. Histopathology showed sloughing of tracheal mucosa in all groups while hypertrophic secretary glands in G1. Lungs showed emphysema in all groups except G4. Kidneys showed glomerulonephritis while Liver showed congestion and hyperemia in all groups. cELISA, revealed the antibody titers rose from 1st to 3rd week post infection afterwards, reduced slowly. Antibody titers were higher in G1 compared to G2 and G3 groups. Conclusion: Mycoplasma mycoides subspecies capri (PG3) can cause infection in goats of all ages, yet the infection is more severe in young animals compared to old.

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Differences in Virulence Between Bovine-Derived Clinical Isolates of Pasteurella multocida Serotype A from the UK and the USA in a Model of Bovine Pneumonic Pasteurellosis

Cited by 5 publications

References 15 publications

L-Ascorbic Acid Shapes Bovine Pasteurella multocida Serogroup A Infection

L-Ascorbic Acid Shapes Bovine Pasteurella multocida Serogroup A Infection

Development of a multiplex PCR for simultaneous detection of Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes

Pathological Study On Experimental Infection With Mycoplasma Mycoides Subspecies Capri In Different Age Groups of Goats

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