Differences in trehalase activity in the intestine of fed and starved larvae of Tenebrio molitor L.

Rosiński, Grzegorz; Wrzeszcz, Alina; Obuchowicz, L.

doi:10.1016/0020-1790(79)90067-2

Cited by 44 publications

(13 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tenebrio molitor L. adults (4 weeks old) were obtained from a culture maintained at the Department of Animal Physiology and Development, Adam Mickiewicz University, Poznań, Poland, as described previously [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the physiological activity of venom from the Eurasian water shrew Neomys fodiens

et al. 2017

Self Cite

View full text Add to dashboard Cite

BackgroundAnimal toxins can have medical and therapeutic applications. Principally, toxins produced by insects, arachnids, snakes and frogs have been characterized. Venomous mammals are rare, and their venoms have not been comprehensively investigated. Among shrews, only the venom of Blarina brevicauda has been analysed so far, and blarina toxin has been proven to be its main toxic component. It is assumed that Neomys fodiens employs its venom to hunt larger prey. However, the toxic profile, properties and mode of action of its venom are largely unknown. Therefore, we analysed the cardio-, myo- and neurotropic properties of N. fodiens venom and saliva of non-venomous Sorex araneus (control tests) in vitro in physiological bioassays carried out on two model organisms: beetles and frogs. For the first time, we fractionated N. fodiens venom and S. araneus saliva by performing chromatographic separation. Next, the properties of selected compounds were analysed in cardiotropic bioassays in the Tenebrio molitor heart.ResultsThe venom of N. fodiens caused a high decrease in the conduction velocity of the frog sciatic nerve, as well as a significant decrease in the force of frog calf muscle contraction. We also recorded a significant decrease in the frog heart contractile activity. Most of the selected compounds from N. fodiens venom displayed a positive chronotropic effect on the beetle heart. However, one fraction caused a strong decrease in the T. molitor heart contractile activity coupled with a reversible cardiac arrest. We did not observe any responses of the insect heart and frog organs to the saliva of S. araneus. Preliminary mass spectrometry analysis revealed that calmodulin-like protein, thymosin β-10, hyaluronidase, lysozyme C and phospholipase A2 are present in the venom of N. fodiens, whereas thymosin β4, lysozyme C and β-defensin are present in S. araneus saliva.ConclusionOur results showed that N. fodiens venom has stronger paralytic properties and lower cardioinhibitory activity. Therefore, it is highly probable that N. fodiens might use its venom as a prey immobilizing agent. We also confirmed that S. araneus is not a venomous mammal because its saliva did not exhibit any toxic effects.Electronic supplementary materialThe online version of this article (10.1186/s12983-017-0230-0) contains supplementary material, which is available to authorized users.

show abstract

Section: Methodsmentioning

confidence: 99%

Evaluation of the physiological activity of venom from the Eurasian water shrew Neomys fodiens

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…A stock culture of T. molitor L. was maintained at the Department of Animal Physiology and Development, Adam Mickiewicz University in Poznań, as described previously by Rosiński et al [37]. Beetles were reared under laboratory conditions at 26 °C and approximately 65% relative humidity under a 12 h light/12 h dark cycle, with oatmeal and fresh carrot being provided as food.…”

Section: Methodsmentioning

confidence: 99%

Cholinergic Agonists and Antagonists Have an Effect on the Metabolism of the Beetle Tenebrio Molitor

2018

Self Cite

View full text Add to dashboard Cite

Synthetic insecticides are still widely used in plant protection. The main target for their action is the nervous system, in which the cholinergic system plays a vital role. Currently available insecticides have low selectivity and act on the cholinergic systems of invertebrates and vertebrates. Acetylcholine, a cholinergic system neurotransmitter, acts on cells by two types of receptors: nicotinic and muscarinic. In mammals, the role of muscarinic acetylcholine receptors (mAChRs) is quite well-known but in insects, is still not enough. Based on data indicating that the muscarinic cholinergic system strongly affects mammalian metabolism, we investigated if it similarly occurs in insects. We investigated the influence of agonists (acetylcholine, carbachol, and pilocarpine) and antagonists (tropane alkaloids: atropine and scopolamine) of mAChRs on the level of selected metabolites in Tenebrio molitor beetle trophic tissues. We analyzed the glycogen content in the fat body and midgut, the total free sugar concentration in the hemolymph and the lipid amount in the fat body. Moreover, we analyzed the levels of insulin-like peptides in the hemolymph. The tested compounds significantly influenced the mentioned parameters. They increased the glycogen content in the fat body and midgut but decreased the concentration of free sugars in the hemolymph. The observed effects were tissue-specific, and were also time- and dose-dependent. We used nonligated and neck-ligated larvae (to eliminate the influence of head factors on tissue metabolism) to determine whether the observed changes are the result of direct or indirect impacts on tissues. The obtained data suggest that the cholinergic system affects the fat body and midgut indirectly and directly and a pleiotropic role for mAChRs exists in the regulation of energy metabolism in insects. Moreover, tested compounds significantly affected the level of insulin-like peptides in hemolymph. Our studies for the first time showed that mAChRs are involved in regulation of insect metabolism of trophic tissues, and act on them directly and indirectly. Improved knowledge about insect cholinergic system may help in searching more selective and environment-friendly solutions in pest management.

show abstract

“…A stock culture of T. molitor was maintained at the Department of Animal Physiology and Development as described previously [31]. Studies were carried out on 4-days old adult male beetles.…”

Section: Insectsmentioning

confidence: 99%

Copper(II) complexes of terminally free alloferon peptide mutants containing two different histidyl (H1 and H6 or H9 or H12) binding sites Structure Stability and Biological Activity

Matusiak

Kuczer

Czarniewska

et al. 2015

Journal of Inorganic Biochemistry

Self Cite

View full text Add to dashboard Cite

Differences in trehalase activity in the intestine of fed and starved larvae of Tenebrio molitor L.

Cited by 44 publications

References 19 publications

Evaluation of the physiological activity of venom from the Eurasian water shrew Neomys fodiens

Evaluation of the physiological activity of venom from the Eurasian water shrew Neomys fodiens

Cholinergic Agonists and Antagonists Have an Effect on the Metabolism of the Beetle Tenebrio Molitor

Copper(II) complexes of terminally free alloferon peptide mutants containing two different histidyl (H1 and H6 or H9 or H12) binding sites Structure Stability and Biological Activity

Contact Info

Product

Resources

About