Differences in the surface composition of seemingly similar F75 cobalt–chromium micron-sized particulates can affect synovial fibroblast viability

Kovacik, M.W.; Mostardi, Richard A.; Neal, Deborah; Bear, T.F.; Askew, Michael J.; Bender, Edward T.; Walker, J.; Ramsier, R. D.

doi:10.1016/j.colsurfb.2008.04.018

Cited by 7 publications

(5 citation statements)

References 37 publications

(47 reference statements)

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“…We did not observe any significant changes in the size or shape of the particles. In addition to the common use of metal-tometal arthroplasty, research [20,39] has revealed that the surface change of the chemical composition on the particle could produce different biological effects on cells. Therefore, we also compared the surface chemical composition between the different endotoxin removal treatments.…”

Section: Discussionmentioning

confidence: 99%

“…In fact, some papers contain no specific methods for the removal of endotoxins from particles. Instead, these studies describe the use of 0.1 lm polycarbonate filter membranes [11][12][13], ethanol [14][15][16][17], autoclaving [14], UV light [14], gamma irradiation [18,19] or sterilization without including detailed descriptions [20,21]. However, these methods do not remove most of the endotoxins from particles.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the cytotoxic and inflammatory responses of titanium particles with different methods for endotoxin removal in RAW264.7 macrophages

Ding

Zhu

Tang

et al. 2012

J Mater Sci: Mater Med

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It is generally accepted that periprosthetic bone resorption is initiated through aseptic inflammation aggravated by wear particles that are generated from artificial joint. However, some studies have demonstrated that "endotoxin-free" wear particles are almost completely unable to stimulate the macrophage-mediated production of proinflammatory cytokines. Here, we compare the titanium particles with different methods of endotoxin removal. The results indicated that different titanium particle preparation dosages did not significantly change particle size, morphology, and chemical composition. But it could cause variations in the endotoxin concentration of titanium particles and inflammatory responses in RAW264.7 macrophages. The particles with higher endotoxin levels correlated with more extensive inflammatory responses. When testing endotoxins using the supernatant of particle suspensions, it would lead to false negative results compared with testing the particle themselves. And when using the particles themselves, all the particles should be removed by centrifugation to avoid particle interference before the absorbance value was determined. Therefore, we suggest that research concerning wear particles should completely describe the endotoxin testing process, including endotoxin removal from particles and the details of endotoxin testing. Moreover, future research should focus on the surface of wear particles (the potential role of adherent endotoxin) rather than the particles themselves.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of the cytotoxic and inflammatory responses of titanium particles with different methods for endotoxin removal in RAW264.7 macrophages

Ding

Zhu

Tang

et al. 2012

J Mater Sci: Mater Med

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“…However, cytotoxicity varies considerably with particle size, particle geometry, and surface phenomenology with severe cellular toxicity caused by CoCr alloys (Kovacik et al. ; Mostardi et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…Most authors showed a dose dependency for the cytotoxic effect of metal particles (Mostardi et al 2010). However, cytotoxicity varies considerably with particle size, particle geometry, and surface phenomenology with severe cellular toxicity caused by CoCr alloys (Kovacik et al 2008;Mostardi et al 2010). Furthermore, hypersensitivity reactions might vary among individuals (Granchi et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Material attrition and bone micromorphology after conventional and ultrasonic implant site preparation

Rashad

Sadr-Eshkevari

Weuster

et al. 2012

Clinical Oral Implants Res

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“…However, the CoCr-A particles were less lethal to fibroblasts than the CoCr-B particles. In a more recent study, 67 the authors explained these results, showing that while the bulk metallic composition of the powders was similar, the surface was completely different, and hence, there were differences in terms of cytotoxicity to fibroblasts in culture. Furthermore, Naji and Harmand 68 showed that by simply altering the surface finishing process, the cytocompatibility of CoCr particles in fibroblasts could change markedly.…”

Section: Laboratory Wear Simulationsmentioning

confidence: 94%

Biological effects of wear particles generated in total joint replacements: trends and future prospects

Suñer

Tipper

Emami

2012

Tribology - Materials, Surfaces & Interfaces

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Joint replacements have considerably improved the quality of life of patients with joints damaged by disease or trauma. However, problems associated with wear particles generated due to the relative motion between the components of the bearing are still present and can lead to the eventual failure of the implant. The biological response to wear debris affects directly the longevity of the prosthesis. The identification of the mechanisms by which cells respond to wear debris and how particles distribute into the human body may provide valuable information for the long term success of artificial joints. During the last few decades, orthopaedic research has been focused on predicting the in vivo performance of joint replacements. However, the exact relationship between material physicochemical properties and inflammatory response has not been fully understood. Laboratory wear simulators provide an accurate prediction of implant wear performance. Though, particles generated from such wear simulators require validation to compare them with particles extracted from peri-implant tissues. This review focuses initially on the current status of total joint replacements (hard on soft and hard on hard bearings) as well as on the tribological behaviour of the potential materials currently under investigation. Then, the correspondence between particles observed in vivo and those generated in vitro to predict the cellular response to wear debris is discussed. Finally, the biological effects of the degradation products generated by wear and corrosion are described.

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Differences in the surface composition of seemingly similar F75 cobalt–chromium micron-sized particulates can affect synovial fibroblast viability

Cited by 7 publications

References 37 publications

Comparison of the cytotoxic and inflammatory responses of titanium particles with different methods for endotoxin removal in RAW264.7 macrophages

Comparison of the cytotoxic and inflammatory responses of titanium particles with different methods for endotoxin removal in RAW264.7 macrophages

Material attrition and bone micromorphology after conventional and ultrasonic implant site preparation

Biological effects of wear particles generated in total joint replacements: trends and future prospects

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