Differences in the regulation of exocellular proteinase synthesis during growth and sporogenesis of Bacillus megaterium

Abstract

Differences in the regulation of exocellular proteinase synthesis during growth and sporogenesis of Bacillus megaterium. Can. J. Microbiol. 28: 1214-121 8. Bacillus megaterium 27 produces one exocellular metalloproteinase during growth as well as during sporogenesis. The formation of the enzyme during growth is repressed by the addition of amino acids of the leucine-isoleucine group. Its synthesis during sporogenesis is rather insensitive to repression. The enzyme is formed during the first 90 min after transfer to a sporulation medium, amino acids delaying only the onset of its synthesis as well as the onset of sporogenesis. The synthesis of the proteinase in asporogenic B. megaterium KM1 is repressed by amino acids during growth as well as during incubation in the sporulation medium and proceeds for a longer time under the latter condition. The results suggest that the formation of the proteinase during growth may be controlled differently from that during sporulation. CHALOUPKA, J., A. I. SEVERIN, K. J. SASTRY, H. KUEEROVA et M. STRNADOVA. 1982. Differences in the regulation of exocellular proteinase synthesis during growth and sporogenesis of Bacillus megaterium. Can. J. Microbiol. 28: 1214-121 8. Bacillus megaterium 27 produit une mttallo-prottinase exocellulaire durant sa croissance tout c o m e durant la sporogenkse.La formation de l'enzyme pendant la croissance est riprimie par l'addition d'acides amints du groupe leucine-isoleucine. Sa synthkse durant la sporogenbe est plutSt insensible h la ripression. L'enzyme est form6 au cours des 90 premikres min aprks le transfert dans un milieu de sporulation; les acides amints retardent seulement le dtclenchement de sa synthkse tout c o m e le diclenchement de la sporogenbse. La synthkse de la prottinase chez l'asporogbne B. megaterium KM1 est riprimie par les acides aminis durant sa croissance ainsi que durant l'incubation dans le milieu de sporulation et sous cette condition, elle se poursuit plus longtemps. Les rtsultats suggkrent que la formation de la prottinase durant la croissance pourrait Etre sous un contr6le qui differe de celui de la sporulation.[Traduit par le journal]

Isolation and characterization of sporulation lacZ fusion mutants of Bacillus megaterium

“…When the wild-type strain and the fusion mutants were tested for protease production, all were found to be positive. The extracellular protease of QM B1551 is completely inhibited by EDTA (data not shown) and therefore is probably the single neutral, or metalloprotease reported for other strains of B. megaterium (Chaloupka et al, 1982;Millet & Aubert, 1969;Priest, 1977). Indeed, we have isolated a mutant that produces no detectable extracellular proteases on milk plates (D. A. Lach & P. S. Vary, unpublished data).…”

“…Sporulating cells have been tested for several enzymes (Chatelain, 1975), fatty acids (Scandella & Kornberg, 1969), polyamines (Setlow, 1974), proteases (Chaloupka et al, 1982;Setlow, 1975), electron transport , calcium accumulation (Bronner & Freund, 1972;Hogarth & Ellar, 1978), dipicolinic acid (DPA) (Bach & Gilvarg, 1966), coat protein synthesis (Imagawa et al, 1985) and penicillin-binding proteins (Todd & Ellar, 1982). Several small acid-soluble proteins (SASPs) and their specific protease have also been isolated, cloned and characterized by Setlow and co-workers.…”

Molecular cloning and nucleotide sequence of the gene encoding a calcium-dependent exoproteinase from Bacillus megaterium ATCC 14581

“…The formation of the enzyme during growth is repressed by free amino acids and glucose or acetate (Chaloupka, 1969;Millet & Aubert, 1969). Its synthesis during sporogenesis is rather insensitive to repression (Chaloupka et a/., 1982).…”

Suppression by temperature of sporulation and of exocellular metalloproteinase synthesis in Bacillus megaterium

“…The level of mRNA coding for the enzyme was determined in the dark in the presence of actinomycin D (1/~g/ml, Calbiochem) at 35°C as described earlier [5]. The enzyme activity released in the presence of a mixture of actinomycin D and tetracycline (50/~g/ml, Spofa, Prague) was taken as the performed enzyme and was subtracted• During a 33-min interval of incubation with actinomycin D more than 95% of the proteinasecoding capacity declined [5].…”

“…Bacillus megaterium ~ excretes the metalloproteinase during growth as well as during sporogenesis [3]. However, during spornlation, the synthesis of the metalloproteinase is much less sensitive to repression and is switched off suddenly after 2 h incubation in the sporulation medium at 35°C [5]. However, during spornlation, the synthesis of the metalloproteinase is much less sensitive to repression and is switched off suddenly after 2 h incubation in the sporulation medium at 35°C [5].…”