Differences in the Microstructure and Biomechanical Properties of the Recurrent Laryngeal Nerve as a Function of Age and Location

Williams, Megan; Utzinger, Urs; Barkmeier-Kraemer, Julie; Geest, Jonathan P. Vande

doi:10.1115/1.4027682

Cited by 13 publications

(15 citation statements)

References 43 publications

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“…The Advanced Intravital Microscope (AIM) for multiphoton imaging at the University of Arizona's BIO5 institute was used to observe the total cell migration along the scaffold depth [21, 22]. The AIM is an Olympus BX51 upright laser-scanning microscope coupled to a Coherent 120-fs tunable pulsed Titanium-Sapphire laser (Santa Clara, CA).…”

Section: Methodsmentioning

confidence: 99%

TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs

et al. 2015

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A main goal of tissue engineering is the development of scaffolds that replace, restore and improve injured tissue. These scaffolds have to mimic natural tissue, constituted by an extracellular matrix (ECM) support, cells attached to the ECM, and signaling molecules such as growth factors that regulate cell function. In this study we created electrospun flat sheet scaffolds using different compositions of gelatin and fibrinogen. Smooth muscle cells (SMCs) were seeded on the scaffolds, and proliferation and infiltration were evaluated. Additionally, different concentrations of Transforming Growth Factor-beta2 (TGFβ2) were added to the medium with the aim of elucidating its effect on cell proliferation, migration and collagen production. Our results demostrated that a scafold with a composition of 80% gelatin-20% fibrinogen is suitable for tissue engineering applications since it promotes cell growth and migration. The addition of TGFβ2 at low concentrations (≤1ng/ml) to the culture medium resulted in an increase in SMC proliferation and scaffold infiltration, and in the reduction of collagen production. In contrast, TGFβ2 at concentrations >1ng/ml inhibited cell proliferation and migration while stimulating collagen production. According to our results TGFβ2 concentration has a differential effect on SMC function and thus can be used as a biochemical modulator that can be beneficial for tissue engineering applications.

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Section: Methodsmentioning

confidence: 99%

TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs

et al. 2015

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“…Given the differences in the species and types of nerves described above, the comparison of quantitative data among studies is challenging. Several studies have been done that demonstrate that the morphology and mechanical behavior of peripheral motor nerves vary among different types of nerves, as well as along the length of an individual nerve (1,4,8,37). However, until function-deficit studies are done in human RLN tissues, these previously published works provide some indication of the quantity of strain required to elicit damage to a peripheral nerve.…”

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confidence: 94%

“…An isotropic, hyperelastic constitutive model was used in our previous work to capture the behavior of porcine RLN tissue (37). Briefly, Cauchy stress, T 11, was calculated at each time point as…”

Section: Materials Propertiesmentioning

confidence: 99%

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A computational study of the role of the aortic arch in idiopathic unilateral vocal-fold paralysis

Williams

Ayylasomayajula

Behkam

et al. 2015

Journal of Applied Physiology

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Unilateral vocal-fold paralysis (UVP) occurs when one of the vocal folds becomes paralyzed due to damage to the recurrent laryngeal nerve (RLN). Individuals with UVP experience problems with speaking, swallowing, and breathing. Nearly two-thirds of all cases of UVP is associated with impaired function of the left RLN, which branches from the vagus nerve within the thoracic cavity and loops around the aorta before ascending to the larynx within the neck. We hypothesize that this path predisposes the left RLN to a supraphysiological, biomechanical environment, contributing to onset of UVP. Specifically, this research focuses on the identification of the contribution of the aorta to onset of left-sided UVP. Important to this goal is determining the relative influence of the material properties of the RLN and the aorta in controlling the biomechanical environment of the RLN. Finite element analysis was used to estimate the stress and strain imposed on the left RLN as a function of the material properties and loading conditions. The peak stress and strain in the RLN were quantified as a function of RLN and aortic material properties and aortic blood pressure using Spearman rank correlation coefficients. The material properties of the aortic arch showed the strongest correlation with peak stress [ρ = -0.63, 95% confidence interval (CI), -1.00 to -0.25] and strain (ρ = -0.62, 95% CI, -0.99 to -0.24) in the RLN. Our results suggest an important role for the aorta in controlling the biomechanical environment of the RLN and potentially in the onset of left-sided UVP that is idiopathic.

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“…Tissues are often mechanically inhomogeneous due to variations in properties (e.g., supraspinatus tendon [1], [2]; pulmonary artery [3]; left recurrent laryngeal nerve [4]), variations in active behavior (e.g., myocardial shortening [5]), injury (e.g., whiplash [6]), myocardial [7]–[9] or dermal [10]–[12] scarring), or disease (coronary artery disease [13]; idiopathic pulmonary fibrosis [14], [15]; or cancer [16], [17]). The imaging of soft tissues to identify regional variations in mechanical properties, known as elastography, has become a major focus for medical imaging (reviewed by [18]–[21]).…”

Section: Introductionmentioning

confidence: 99%

Automatic Segmentation of Mechanically Inhomogeneous Tissues Based on Deformation Gradient Jump

Witzenburg

Dhume

Lake

et al. 2016

IEEE Trans. Med. Imaging

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Variations in properties, active behavior, injury, scarring, and/or disease can all cause a tissue’s mechanical behavior to be heterogeneous. Advances in imaging technology allow for accurate full-field displacement tracking of both in vitro and in vivo deformation from an applied load. While detailed strain fields provide some insight into tissue behavior, material properties are usually determined by fitting stress-strain behavior with a constitutive equation. However, the determination of the mechanical behavior of heterogeneous soft tissue requires a spatially varying constitutive equation (i.e. one in which the material parameters vary with position). We present an approach that computationally dissects the sample domain into many homogeneous subdomains, wherein subdomain boundaries are formed by applying a betweenness based graphical analysis to the deformation gradient field to identify locations with large discontinuities. This novel partitioning technique successfully determined the shape, size and location of regions with locally similar material properties for: (1) a series of simulated soft tissue samples prescribed with both abrupt and gradual changes in anisotropy strength, prescribed fiber alignment, stiffness, and nonlinearity, (2) tissue analogs (PDMS and collagen gels) which were tested biaxially and speckle tracked (3) and soft tissues which exhibited a natural variation in properties (cadaveric supraspinatus tendon), a pathologic variation in properties (thoracic aorta containing transmural plaque), and active behavior (contracting cardiac sheet). The routine enables the dissection of samples computationally rather than physically, allowing for the study of small tissues specimens with unknown and irregular inhomogeneity.

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Differences in the Microstructure and Biomechanical Properties of the Recurrent Laryngeal Nerve as a Function of Age and Location

Cited by 13 publications

References 43 publications

TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs

TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs

A computational study of the role of the aortic arch in idiopathic unilateral vocal-fold paralysis

Automatic Segmentation of Mechanically Inhomogeneous Tissues Based on Deformation Gradient Jump

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