Differences in the bone differentiation properties of MC3T3‐E1 cells on polished bulk and sputter‐deposited titanium specimens

Oya, Kei; Tanaka, Yuta; Moriyama, Yoshihisa; Yoshioka, Yuki; Kimura, Tsuyoshi; Tsutsumi, Yusuke; Doi, H.; Nomura, Naoyuki; Noda, Kohei; Kishida, Akio; Hanawa, Takao

doi:10.1002/jbm.a.32751

Cited by 12 publications

(11 citation statements)

References 21 publications

(26 reference statements)

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“…Considering their specific roles during osteogenic differentiation and calcification, target genes can be divided into two groups: one includes Runx2, Col1α1, and Akp2, which are involved in osteoblast differentiation; and the other includes Ibsp, Spp1, Bglap1, and Ifitm5, which are involved in cellular calcification, also known as mineralization. Additionally, in our previous study, the maximum alkaline phosphatase activity of cells occurred between days 10 and 14, which may indicate the transition from initial osteogenic differentiation to calcification after the maturation of osteoblast cells. Analysis of osteogenic differentiation and calcification‐involved gene expression on the metals was comprised of two parts: osteogenic differentiation during the first 7 days of culture (Fig.…”

Section: Discussionmentioning

confidence: 67%

Differences in the calcification of preosteoblast cultured on sputter‐deposited titanium, zirconium, and gold

Chen

Nagai

Tsutsumi

et al. 2015

J Biomedical Materials Res

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In this study, osteogenic differentiation and calcification of preosteoblast (MC3T3-E1) cultured on sputter-deposited titanium (Ti), zirconium (Zr), and gold (Au) on cover glasses were evaluated to understand the differences in bone formation ability among these three metals; these metals show the same high corrosion resistance, but Ti and Zr are covered by surface passive oxide film while Au is not covered by the oxide film. Ti and Zr promoted cellular proliferation without osteogenic differentiation. Cells cultured on Ti and Zr expressed higher levels of Runx2, Col1α1, and Akp2 at an earlier stage, which indicated faster promotion of osteogenic differentiation, as compared to those cultured on Au. Moreover, after 21 days of culture, the Bglap1 and Ifitm5 expression peaks in cells cultured on Ti and Zr were higher than those in cells cultured on Au, which indicated faster promotion of calcification. Cells cultured on Ti showed an advantage in osteogenic differentiation at an early stage, while cells on Zr showed better calcification promotion with a long-term culture. The amount of extracellular calcified deposits was in good agreement with the gene expression results. On the other hand, the intracellular calcium content of cells on Au specimens was higher than that of cells on Ti and Zr specimens. The results indicate that preosteoblasts on Ti and Zr showed faster osteogenic differentiation and calcification than those on Au, whereas Au improved the intracellular calcium content. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 639-651, 2016.

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Section: Discussionmentioning

confidence: 67%

Differences in the calcification of preosteoblast cultured on sputter‐deposited titanium, zirconium, and gold

Chen

Nagai

Tsutsumi

et al. 2015

J Biomedical Materials Res

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“…In addition, the surface of pure titanium is stabilized by forming a thin oxide film of approximately 2-5 nm in thickness consisting of TiO 2 , TiO, and Ti 2 O 3 . 25,26) The thin oxide film may affect the processing of the nanoperiodic structure. Further analyses and consideration regarding factors affecting laser processing are required.…”

Section: Discussionmentioning

confidence: 99%

Morphological Observations of Mesenchymal Stem Cell Adhesion to a Nanoperiodic-Structured Titanium Surface Patterned Using Femtosecond Laser Processing

Oya

Aoki

Shimomura

et al. 2012

Jpn. J. Appl. Phys.

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It is known that the adhesive and anisotropic properties of cell-derived biomaterials are affected by micro-or nanoscale structures processed on culture surfaces. In the present study, the femtosecond laser processing technique was used to scan a laser beam at an intensity of approximately the ablation threshold level on a titanium surface for nanoscale processing. Microscopy observation revealed that the processed titanium exhibited a periodic-patterned groove structure at the surface; the width and depth of the groove were 292 AE 50 and 99 AE 31 nm, respectively, and the periodic pitch of the groove was 501 AE 100 nm. Human synovium-derived mesenchymal stem cells were cultured on the surface at a cell density of 3:0 Â 10 3 cells/cm 2 after 4 cell passages. For comparison, the cells were also cultured on a nonprocessed titanium surface under the condition identical to that of the processed surface. Results revealed that the duration for cell attachment to the surface was markedly reduced on the processed titanium as compared with the nonprocessed titanium. Moreover, on the processed titanium, cell extension area significantly increased while cell orientation was aligned along the direction of the periodic grooves. These results suggest that the femtosecond laser processing improves the adhesive and anisotropic properties of cells by producing the nanoperiodic structure on titanium culture surfaces.

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“…A cylinder of bulk cpTi placed in bone demonstrated hypocellular fibrous tissue surrounding the implant with little inflammation . MC3T3‐E1 preosteoblast cells do not exhibit cytotoxicity when cultured on cpTi unless a cathodic voltage is applied . As bulk, the advantage of Ti‐alloy as a stem is lower modulus of elasticity, and its disadvantage as a head is poor wear resistance .…”

Section: Metals Utilized During Tjrmentioning

confidence: 99%

The biological response to orthopaedic implants for joint replacement: Part I: Metals

Gibon

Amanatullah

Loi

et al. 2016

J. Biomed. Mater. Res.

107

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Joint replacement is a commonly performed, highly successful orthopaedic procedure, for which surgeons have a large choice of different materials and implant designs. The materials used for joint replacement must be both biologically acceptable to minimize adverse local tissue reactions, and robust enough to support weight bearing during common activities of daily living. Modern joint replacements are made from metals and their alloys, polymers, ceramics, and composites. This review focuses on the biological response to the different biomaterials used for joint replacement. In general, modern materials for joint replacement are well tolerated by the body as long as they are in bulk (rather than in particulate or ionic) form, are mechanically stable and noninfected. If the latter conditions are not met, the prosthesis will be associated with an acute/chronic inflammatory reaction, peri-prosthetic osteolysis, loosening and failure. This article (Part 1 of 2) is dedicated to the use of metallic devices in orthopaedic surgery including the associated biological response to metallic byproducts is a review of the basic science literature regarding this topic.

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Differences in the bone differentiation properties of MC3T3‐E1 cells on polished bulk and sputter‐deposited titanium specimens

Cited by 12 publications

References 21 publications

Differences in the calcification of preosteoblast cultured on sputter‐deposited titanium, zirconium, and gold

Differences in the calcification of preosteoblast cultured on sputter‐deposited titanium, zirconium, and gold

Morphological Observations of Mesenchymal Stem Cell Adhesion to a Nanoperiodic-Structured Titanium Surface Patterned Using Femtosecond Laser Processing

The biological response to orthopaedic implants for joint replacement: Part I: Metals

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