Differences in the Bacteriome of Smokeless Tobacco Products with Different Oral Carcinogenicity: Compositional and Predicted Functional Analysis

Al-Hebshi, Nezar N.; Alharbi, Fahd; Mahri, Mohammed; Chen, Tsute

doi:10.20944/preprints201703.0030.v1

Cited by 10 publications

(12 citation statements)

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“…16S rRNA gene library preparation and sequencing were done at the Australian Center for Ecogenomics (Brisbane, Australia) as detailed previously (Al-Hebshi et al, 2017a). Briefly, the V1-3 region was amplified using the degenerate primers 27FYM (Frank et al, 2008) and 519R (Lane et al, 1985) in standard PCR conditions.…”

Section: S Rrna Sequencing and Bioinformatic Analysismentioning

confidence: 99%

Salivary Oral Microbiome of Children With Juvenile Idiopathic Arthritis: A Norwegian Cross-Sectional Study

Frid

Baraniya

Halbig

et al. 2020

Front. Cell. Infect. Microbiol.

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Section: S Rrna Sequencing and Bioinformatic Analysismentioning

confidence: 99%

Salivary Oral Microbiome of Children With Juvenile Idiopathic Arthritis: A Norwegian Cross-Sectional Study

Frid

Baraniya

Halbig

et al. 2020

Front. Cell. Infect. Microbiol.

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“…28,29 It is heavily contaminated with microorganisms which likely contribute to carcinogenesis. 30 Khat is a psychoactive stimulant derived from the leaves of the Catha edulis plant, which is a flowering evergreen shrub indigenous to East Africa and parts of the Middle East. 31 Khat (or Miraa as it known in Kenya) holds significant social and cultural importance, particularly in Eastern Africa and the Arabian Peninsula, with increasing consumption in the global diaspora.…”

Section: Aetiology and Risk Factors Of Oral Cancer In Africamentioning

confidence: 99%

The burden of oral cancer in sub-Saharan Africa

Hille

Johnson

2017

Translational Research in Oral Oncology

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Objectives: To describe incidence and mortality rates of oral and of oropharyngeal cancers in sub-Saharan Africa using available sources and to discuss shortcomings. Methods: Incidence and mortality rates were obtained from GLOBOCAN online (IARC 2012) and the pathology-based South African National Cancer Registry (SA-NCR). Descriptions of oral and of oropharynx cancers for the four main geographical regions were derived from the literature. For South Africa, the incidence by ethnic group of lip and intra-oral cancer over the period 1988-2012 could be calculated from SA-NCR. The age distributions for intraoral and for oropharynx cancer in both genders in South Africa were compared for the period 1996-2002. Results: Age standardized incidence rates and mortality rates of lip and of oral cancer in sub-Saharan Africa varied substantially, depending on the regions, the mix of carcinogenic substances consumed in each country and the quality of the cancer registry data. African islands in the Indian Ocean and the Southern and Eastern African countries report the highest rates; these are generally reported to be much lower in West African countries. Available data are now old: not beyond 2010. Up until that time, in the Republic of South Africa, there appears to have been a slight decline in oral cancer rates, except in Black females, but a rise this millennium in cancer of the oropharynx, especially in Coloureds (mixed-race). At least until 2002, the Human Immunodeficiency Virus infection (HIV) pandemic had little or no stimulating influence on the incidence rates of oral squamous carcinoma in South Africa, although Kaposi's sarcoma has been a major burden in the past. Asian and mixed-race South African females had the highest levels of oral squamous cell carcinomas in the <45 year age group. Conclusion:The quality of cancer registry data in sub-Saharan Africa is variable, unreliable and out of date, suggesting gross under-ascertainment. Records of all causes of death are poor, which can result in both under-and over-attribution of cancer mortality. Improvements of the necessary resources for cancer registration and death certification deserve a high priority.

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“…Library preparation and sequencing were done at the Australian Centre for Ecogenomics as described previously 25 . Briefly, the degenerate primers 27FYM …”

Section: Amplicon Library Preparation and Sequencingmentioning

confidence: 99%

“…PRJNA352375 and preprocessed as described previously 25 . Briefly, reads with primer mismatches were removed before the primer sequences were trimmed off.…”

Section: Preprocessing Of Sequencing Datamentioning

confidence: 99%

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Inflammatory Bacteriome Featuring Fusobacterium nucleatum and Pseudomonas aeruginosa Identified in Association with Oral Squamous Cell Carcinoma

Al-Hebshi¹,

Nasher²,

Maryoud³

et al. 2017

Preprint

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Abstract:Studies on the possible association between bacteria and oral squamous cell carcinoma (OSCC) remain inconclusive, largely due to methodological variations/limitations. The objective of this study was to characterize the species composition as well as functional attributes of the bacteriome associated with OSCC. DNA obtained from 20 fresh OSCC biopsies (cases) and 20 deep-epithelium swabs (matched control subjects) were sequenced for the V1-V3 region using Illumina's 2x300 bp chemistry. High quality, non-chimeric merged reads were classified to species level using a prioritized BLASTN-algorithm. Downstream analyses were performed using QIIME, PICRUSt, and LEfSe. Fusobacterium nucleatum subsp. polymorphum was the most significantly overrepresented species in the tumors followed by Pseudomonas aeruginosa and Campylobacter sp. Peer-reviewed version available at Scientific Reports 2017, 7, , 1834; doi:10.1038/s41598-017-02079-3 2 while those responsible for DNA repair and combination, purine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, ribosome biogenesis and glycolysis/gluconeogenesis were significantly associated with the controls. This is the first epidemiological evidence for association of F. nucleatum and P. aeruginosa with OSCC. Functionally, an "inflammatory bacteriome" is enriched in OSSC.

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Differences in the Bacteriome of Smokeless Tobacco Products with Different Oral Carcinogenicity: Compositional and Predicted Functional Analysis

Cited by 10 publications

References 40 publications

Salivary Oral Microbiome of Children With Juvenile Idiopathic Arthritis: A Norwegian Cross-Sectional Study

Salivary Oral Microbiome of Children With Juvenile Idiopathic Arthritis: A Norwegian Cross-Sectional Study

The burden of oral cancer in sub-Saharan Africa

Inflammatory Bacteriome Featuring <em>Fusobacterium nucleatum</em> and <em>Pseudomonas aeruginosa </em>Identified in Association with Oral Squamous Cell Carcinoma<strong></strong>

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