Differences in the Adsorptive Behavior of Human Strains of
            <i>Actinomyces viscosus</i>
            and
            <i>Actinomyces naeslundii</i>
            to Saliva-Treated Hydroxyapatite Surfaces

Qureshi, J. V.; Gibbons, R. J.

doi:10.1128/iai.31.1.261-266.1981

Cited by 28 publications

(15 citation statements)

References 12 publications

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“…However, neuramitiidase treatment of saliva barely enhanced agglutination in the absence of P-Gal, casting further doubt on the relative importance of P-Gal binding in Actinomyces agglutination. These findings have parallels in QtiRESHi & GIBBONS' studies of Actinomyces attachment to saliva-coated hydroxyapatite (8). These workers reported only weakly inhibitory effects of galactose and lactose and rather inconsistent neuraminidase enhancement.…”

Section: Anti-anti-buffersupporting

confidence: 68%

“…It is feasible that some pellicleassociated molecules for which A. viscosus has afTmity during its attachment to teeth are derived from the pool of agglutinins in saliva. Agglutinating activity could be ab.sorbed IW)m saliva by incubation with hydroxyapatite to mitnic the coating procedure employed by QiiRKsm & GIBBONS (8). Previous findings that streptococcal agglutinins can sorb to hydroxyapatite (19) and be extracted from dental plaque (20) are consistetit with this concept.…”

Section: Anti-anti-buffermentioning

confidence: 97%

“…Actinomycrs vi.scosus and A. naeskmdii differ in their intraoral ecologies (7), in their abilities to attach to saliva-coated hydroxyapatite (4,8), to hemagglutinate and to prime RBG for hemagglutination (5), and in the antigenic composition of their .surface fibrils (9,10). It follows that differences in the extent to which they interact with salivary agglutinins may be anticipated.…”

mentioning

confidence: 99%

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Actinomyces viscosus and Actinomyces naeslundii agglutinins in human saliva

Ellen

Bratthall

Borgström

et al. 1983

European J Oral Sciences

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– The objectives were to determine the degree of Actinomyces agglutinating activity in human saliva and to begin characterizing the agglutination mechanism. Agglutination titres of whole saliva collected from adults and 6‐yr‐old children were compared. Titres for A. naeslundii were always higher than for A. viscosus. The mean A. naeslundii titre for the adults’ and children's samples were equivalent. The children had a slightly lower mean titre than the adults for A. viscosus. No correlation was found between IgA concentration and agglutination titre. Agglutinating activity was partially impaired by incubation with anti‐IgA serum. Activity in submandibular/sublingual saliva was resistant to heat at 56°C but sensitive to boiling. Boiling the bacteria had no effect. In sugar inhibition tests, only galactosides (β‐Gal) and glucosamine (for A. viscosus) affected Actinomyces agglutination but impairment was only temporary. Agglutinating activity was diminished by incubating saliva with hydroxyapatite. Thus, Actinomyces agglutinins 1) are probably distinct from IgA but may complex with it; 2) may include both β‐Gal and higher affinity sites; and 3) may contribute to salivary pellicle.

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Section: Anti-anti-buffersupporting

confidence: 68%

Section: Anti-anti-buffermentioning

confidence: 97%

mentioning

confidence: 99%

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Actinomyces viscosus and Actinomyces naeslundii agglutinins in human saliva

Ellen

Bratthall

Borgström

et al. 1983

European J Oral Sciences

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“…Furthermore, the pretreatment of hydroxyapatite treated with human saliva with neuraminidase did not increase the attachment of strain 5951. This finding conflicts with data presented by Qureshi & Gibbons (32), in which type 2-bearing strains of A. naeslundii showed somewhat enhanced attachment to neuraminidasetreated hydroxyapatite treated with human saliva. The disparity may reflect differences in the quantity of hydroxyapatite used between comparative assay systems as presented by these authors.…”

Section: Discussioncontrasting

confidence: 99%

Fimbrial‐mediated colonization of murine teeth by Actinomyces naeslundii

Beem¹,

Hurley²,

Nesbitt³

et al. 1996

Oral Microbiology and Immunology

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Groups of mice fed diets high in sucrose or glucose were orally inoculated with 10(10), 10(9) or 10(8) colony-forming units of one of the following Actinomyces naeslundii strains possessing the type 1 (T1+) and/or the type 2 (T2+) fimbriae: T14VJ1 (T1+, T2+), 5519 (T1+), 5951 (T2+), and 147 (non-fimbriated). Ninety-six hours after inoculation their upper jaws were cultured to look at the implantation of each of these strains on the teeth. In mice fed a sucrose diet, regardless of the presence or absence of fimbriae, each bacterial strain colonized 100% of the mice at the highest inoculation doses of the infecting organism. But at a dose of 10(8), T14V-J1 was the only strain which colonized 100% (12/12) of the mice, 5519 colonized 10/11, 5951 colonized 9/11 and 147 colonized 7/11. These differences were not statistically significant. When mice were fed a high-glucose diet, 100% infection was achieved with strains T14V-J1, 5519 and 5951 only at the highest dose of 10(10) colony-forming units. Strain 147 colonized in 8/9 of the mice at that dosage. At lower dosages, no bacterial strain implanted in 100% of the mice. In the glucose experiment at a dose of 10(8), strains expressing the T1 fimbriae implanted significantly better than strains without the T1 fimbriae. At a dose of 10(9) colony-forming units, the parent strain T14V-J1 implanted significantly better than strains without the T1 fimbriae. Similarly, strain 5519 (T1+) implanted significantly better than 5951 and implanted better than 147, although the difference was not significant. These results suggest that while the presence of the T1 and T2 fimbriae may confer some advantage in the establishment of these organisms in vivo, even the strains without fimbriae were able to colonize. Strains T14VJ1 and 5519 were found to bind well to hydroxyapatite treated with mouse saliva, while strains 5951 and 147 did not. Only T2 fimbriated strains T14V-J1 and 5951 exhibited a lactose-reversible coaggreation with indigenous strains of enterococci that may contribute to the elevated levels of colonization of strain 5951 in vivo.

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“…The oral species of Actinomvces differ in their distribution and establishment patterns in the oral cavity (8), A. naeslundii is present in pre-dentate subjects on the tongue, mucosa and in saliva, whereas A. visco.sus, currently reclassified in the genospecies A. tmeslundii (18). is present in large numbers only after tooth eruption (8) as a consequence of its adhesion to the pellicle (24) and coaggregation with other bacteria (2,35).…”

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confidence: 99%

Surface‐associated properties of Actinomyces strains and their potential relation to pathogenesis

Loo

Willcox

Knox

1994

Oral Microbiology and Immunology

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Twenty-nine strains from the Actinomyces species were tested for a range of surface properties. Results show considerable heterogeneity both between different species and within some of the species, especially Actinomyces naeslundii. Two commonly used A. naeslundii strains, T14V and ATCC 12104, fell within the low (salivary aggregation and collagen binding by T14V), moderate (surface charge and haemagglutination) or high range of values (hydrophobicity, saliva-coated hydroxyapatite adhesion, polystyrene binding by T14V, fibrinogen binding by T14V and collagen binding by A. naeslundii ATCC 12104). Both strains adhered well to saliva-coated hydroxyapatite; T14V bound the highest amount of fibrinogen, ATCC 12104 had the highest number of cells bound to collagen and T14V was not bound at all. The heterogeneity of these characteristics highlights the need to include a range of strains of Actinomyces in studies on their pathogenicity. Statistical correlations were found between a number of properties, for example saliva-coated hydroxyapatite adhesion and hydrophobicity, and between haemagglutination and hydrophobicity.

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Differences in the Adsorptive Behavior of Human Strains of Actinomyces viscosus and Actinomyces naeslundii to Saliva-Treated Hydroxyapatite Surfaces

Cited by 28 publications

References 12 publications

Actinomyces viscosus and Actinomyces naeslundii agglutinins in human saliva

Actinomyces viscosus and Actinomyces naeslundii agglutinins in human saliva

Fimbrial‐mediated colonization of murine teeth by Actinomyces naeslundii

Surface‐associated properties of Actinomyces strains and their potential relation to pathogenesis

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