Differences in Surface-Exposed Antigen Expression between Helicobacter pylori Strains Isolated from Duodenal Ulcer Patients and from Asymptomatic Subjects

Thoreson, Ann-Catrin E.; Hamlet, A.; Çelik, Janet; Byström, Mona; Nyström, Staffan; Olbe, L; Svennerholm, Ann‐Mari

doi:10.1128/jcm.38.9.3436-3441.2000

Cited by 40 publications

(20 citation statements)

References 34 publications

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“…This agrees well with a previous study on the effect of different H. pylori strains on cytokine and chemokine production in human endothelial cells (HUVEC) [23]. As in that study, we found no correlation between cytokine production and CagA or the Lewis y phenotype, all suggested to be of importance in the pathogenesis of H. pylori infection [17]. All strains in our study expressed cagA , which has been shown to be of major importance for induction of IL‐8 production [24–26].…”

Section: Discussionsupporting

confidence: 93%

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Down-regulation of epithelial IL-8 responses inHelicobacter pylori-infected duodenal ulcer patients depends on host factors, rather than bacterial factors

Stromberg¹,

Edebo

Lundin³

et al. 2005

Clinical and Experimental Immunology

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SummaryHelicobacter pylori infection is one of the most common gastrointestinal infections worldwide. Although the majority of the infected individuals remain asymptomatic carriers of the bacteria, approximately 15% develop peptic ulcers, which are most prevalent in the duodenum. H. pylori induce a vigorous immune response which, however, fails to clear the infection. Instead, the chronic inflammation that arises in the infected gastroduodenal mucosa may be involved in the development of H. pylori-associated peptic ulcers. We have previously shown that duodenal ulcer (DU) patients have a significantly lower epithelial cytokine, e.g. IL-8, response in the duodenum than asymptomatic (AS) carriers. In this study we have further investigated the mechanisms behind this finding, i.e. whether it can be explained by bacterial factors, down-regulation of epithelial cytokine production by regulatory T cells, or an impaired ability of the duodenal epithelium in DU patients to produce cytokines. Gastric AGS, and intestinal T84 epithelial cell lines were stimulated with H. pylori strains isolated from DU patients and AS carriers, respectively. All strains were found to induce comparable cytokine and cytokine receptor expression in epithelial cells. Regulatory T cells (CD4+ + + + CD25 high ), isolated from human peripheral blood and cocultured with H. pylori stimulated AGS cells, were found to slightly suppress H. pylori -induced epithelial cytokine production. Furthermore, primary cultures of duodenal epithelial cells from DU patients were found to produce markedly lower amounts of cytokines than epithelial cells isolated from AS carriers. These results suggest that the lower epithelial cytokine responses in the duodenum of DU patients, which may be of importance for the pathogenesis of H. pylori-induced duodenal ulcers, most likely can be explained by host factors, i.e. mainly a decreased ability of the duodenal epithelium to produce cytokines, but possibly partly also down-regulation by regulatory T cells.

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Section: Discussionsupporting

confidence: 93%

“…All H. pylori strains were analysed for cagA genotypes by PCR (Table 1), as previously described [15]. Expression of Lewis blood group antigens (Le x , Le y ) was determined by a whole‐cell ELISA as previously described [17].…”

Section: Methodsmentioning

confidence: 99%

Down-regulation of epithelial IL-8 responses inHelicobacter pylori-infected duodenal ulcer patients depends on host factors, rather than bacterial factors

Stromberg¹,

Edebo

Lundin³

et al. 2005

Clinical and Experimental Immunology

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“…Further, biopsy based studies demonstrated that, in H. pylori infected patients, babA2 was significantly correlated with higher degrees of mucosal inflammation, glandular atrophy and intestinal metaplasia [14,17]. Thoreson et al [18] reported a higher prevalence of BabA in Swedish patients with doudenal ulcer or duodenitis compared to asymptomatic subjects. Yu et al [19] reported that in Yantai County, where the incidence of gastric cancer is among the highest in China, the babA2 + strains were present in 79.8% of the studied patients, and were associated with higher degrees of lymphocytic infiltration, glandular atrophy and intestinal metaplasia.…”

Section: Discussionmentioning

confidence: 98%

Correlation of theHelicobacter pyloriadherence factor BabA with duodenal ulcer disease in four European countries

Olfat

Zheng²,

Oleastro

et al. 2005

FEMS Immunology &Amp; Medical Microbiology

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Helicobacter pylori strains harboring the vacAs1, cagA and babA2 have been associated with ulcer disease (UD). We compared the prevalence of these different genotypes and adhesive properties in H. pylori infected patients with UD in four European countries. Genomic DNA was isolated from 314 H. pylori strains: Germany (GER; n=92), Sweden (SWE, n=74), Portugal (POR, n=91) and Finland (FIN, n=57). The frequencies of babA2 genotype varied from 35% to 60%. Triple-positive strains (vacAs1+, cagA+ and babA2+) were significantly associated with UD in GER and POR and were closely correlated with UD in FIN, but not in SWE. Classification as triple-positive strains had a higher specificity for detection of UD in GER, POR and FIN than type1 or cagA+ strains. In vitro adhesion assays revealed that Swedish strains showed high adhesion properties and were thus correlated with the diagnosis of UD, although PCR detected the babA2 gene at lower frequencies and failed to show a correlation with UD. This finding appears to reflect allelic variations of the babA2 gene in SWE, although adhesive properties of the strains are retained.

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“…Mab 108:3 used for affinity purification was raised against purified rHpaA (28) and Mab HP30‐1:1:6 (used for cloning of hpaA ) was raised against the native protein. These Mabs were compared in a direct ELISA for binding to both the affinity‐purified rHpaA and the native HpaA.…”

Section: Resultsmentioning

confidence: 99%

“…A monoclonal antibody, 108:3 (isotype IgG1, 56 μg/ml), obtained after immunisation of BALB/c mice with rHpaA as described earlier (28), was coupled to NHS‐activated sepharose (Amersham‐Pharmacia, Biotech, Sweden) according to the manufacturer's protocol. The column was equilibrated with buffer (50 mM Tris‐HCl, 0.5 M NaCl, pH 8.0).…”

Section: Methodsmentioning

confidence: 99%

Recombinant HpaA purified from Escherichia coli has biological properties similar to those of native Helicobacter pylori HpaA

Lundström

Bölin

Byström

et al. 2003

APMIS

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The aim of this study was to recombinantly produce and purify Helicobacter pylori adhesin A (HpaA) from Escherichia coli and compare it to purified native H. pylori HpaA, for potential use as a vaccine antigen. The hpaA gene was cloned from H. pylori, transferred to two different expression vectors, and transformed into E. coli. Expression of rHpaA was analysed by immunoblot, inhibition ELISA, and semi-quantitative dot-blot. Using affinity chromatography, rHpaA was purified from E. coli and native HpaA from H. pylori. The binding of both purified proteins to sialic acid was analysed and antibody titres to native and rHpaA were compared after intraperitoneal immunisation of C57/Bl mice. The rHpaA protein was highly expressed in E. coli from both vectors. Purified recombinant and native HpaA bound similarly to fetuin but also to the non-sialylated asialofetuin. Both native HpaA and rHpaA induced comparable amounts of specific antibodies in serum after immunisation and they were identical in double immunodiffusion. In conclusion, rHpaA was successfully produced in E. coli. Purified rHpaA showed biological properties similar to those of native HpaA isolated from H. pylori and may therefore be further used as an antigen in the development of a vaccine against H. pylori infection.

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Differences in Surface-Exposed Antigen Expression between Helicobacter pylori Strains Isolated from Duodenal Ulcer Patients and from Asymptomatic Subjects

Cited by 40 publications

References 34 publications

Down-regulation of epithelial IL-8 responses inHelicobacter pylori-infected duodenal ulcer patients depends on host factors, rather than bacterial factors

Down-regulation of epithelial IL-8 responses inHelicobacter pylori-infected duodenal ulcer patients depends on host factors, rather than bacterial factors

Correlation of theHelicobacter pyloriadherence factor BabA with duodenal ulcer disease in four European countries

Recombinant HpaA purified from Escherichia coli has biological properties similar to those of native Helicobacter pylori HpaA

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