Differences in staining of the unicellular algae Chlorococcales as a function of algaenan content

Zych, Maria; Burczyk, J.; Kotowska, Martyna M.; Kapuścik, A.; Banaś, Antoni; Stolarczyk, A.; Dudek, Sławomir; Klasik, S.

doi:10.1556/aagr.57.2009.3.12

Cited by 12 publications

(12 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To proof the selection of strains, Crystal Violet, Calcofluor, Ruthenium red, Toluidine blue O and FD4-staining were performed to characterize the investigated coccoid strains with respect to cell wall properties. These findings support the work of Kodner et al (2009) and Zych et al (2009) who showed algaenan as present in the species A. obliquus . Also for D. armatus the presence of algaenan was demonstrated before ( Allard and Templier, 2000 ).…”

Section: Discussionsupporting

confidence: 92%

“…The investigated species were selected based on literature research for presence or absence of algaenan ( Table 1 ). In addition, cell wall resistance for all investigated green algae was evaluated again by (a) cell inner staining with Crystal Violet, proposed by Zych et al (2009) , Ruthenium red and Toluidine blue O (b) by estimating the ratio of flow cytometrically measured Calcofluor-fluorescence to Forward-Scatter-signal and (c) exemplary flow cytometric detection of accumulated empty cell walls in the culture ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…Crystal Violet is a common staining dye for Gram-positive and Gram-negative bacteria. Zych et al (2009) suggested that the dye passively penetrates the cell wall of non-algaenan species, while algaenan-containing cell walls exclude the large molecules of Crystal Violet (MW 407.5 g mol -1 ). The authors noted that Crystal Violet remained in the non-algaenan containing algal cells, although rinsing was performed.…”

Section: Resultsmentioning

confidence: 99%

“…Crystal Violet staining was prepared following the protocol of Zych et al (2009) . As modification, concentration of Crystal Violet was reduced to 0.2 g L -1 final concentration instead of 2 g L -1 .…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Cell Wall Structure of Coccoid Green Algae as an Important Trade-Off Between Biotic Interference Mechanisms and Multidimensional Cell Growth

Dunker

Wilhelm

2018

Front. Microbiol.

View full text Add to dashboard Cite

Coccoid green algae can be divided in two groups based on their cell wall structure. One group has a highly chemical resistant cell wall (HR-cell wall) containing algaenan. The other group is more susceptible to chemicals (LR-cell wall – Low resistant cell wall). Algaenan is considered as important molecule to explain cell wall resistance. Interestingly, cell wall types (LR- and HR-cell wall) are not in accordance with the taxonomic classes Chlorophyceae and Trebouxiophyceae, which makes it even more interesting to consider the ecological function. It was already shown that algaenan helps to protect against virus, bacterial and fungal attack, but in this study we show for the first time that green algae with different cell wall properties show different sensitivity against interference competition with the cyanobacterium Microcystis aeruginosa. Based on previous work with co-cultures of M. aeruginosa and two green algae (Acutodesmus obliquus and Oocystis marssonii) differing in their cell wall structure, it was shown that M. aeruginosa could impair only the growth of the green algae if they belong to the LR-cell wall type. In this study it was shown that the sensitivity to biotic interference mechanism shows a more general pattern within coccoid green algae species depending on cell wall structure.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Cell Wall Structure of Coccoid Green Algae as an Important Trade-Off Between Biotic Interference Mechanisms and Multidimensional Cell Growth

Dunker

Wilhelm

2018

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The differential sensitivity of green algae species to allelopathic effects is often discussed in ecotoxicological studies (Chalifour et al, 2009) and might depend on the cell wall composition. Zych et al (2009) found evidence that the permeability of the cell wall is greater in strains with a tri-laminar structure and which do not contain algaenan. O. marsonii is known to possess a tri-laminar cellulose cell wall (Quader, Robinson & van Kempen, 1983;Fujino & Itoh, 1994;Krienitz & Bock, 2012), whereas S. obliquus contains algaenan in its cell wall (Atkinson, Gunning & John, 1972).…”

Section: Co-cultivation Of M Aeruginosa-o Marsoniimentioning

confidence: 97%

Contrasting effects of the cyanobacterium Microcystis aeruginosa on the growth and physiology of two green algae, Oocystis marsonii and Scenedesmus obliquus, revealed by flow cytometry

2013

View full text Add to dashboard Cite

Summary Aquatic photosynthetic productivity is influenced by resource availability, resource use efficiency and interspecific interactions. In hypertrophic environments, interspecific interference is known to influence species succession. The effect of interference by phytoplankton on biomass production is very poorly understood. One hypothesis is that the development of cyanobacterial blooms is triggered by the excretion of allelopathic substances that inhibit the growth of other phytoplankton species. In this study, we analysed how the co‐cultivation of the cyanobacterium Microcystis aeruginosa with two green algae (Oocystis marsonii and Scenedesmus obliquus) influences the resource use efficiency of each partner under nutrient‐replete conditions. Using single‐cell technologies, it was possible to quantify the efficiency of light use and the biomass gain for all three species under hypertrophic (non‐limiting nutrient) conditions. The presence of M. aeruginosa resulted in growth inhibition, reduced metabolic activity and an increased quantum requirement for biomass assimilation in O. marsonii, whereas no changes were observed in S. obliquus. M. aeruginosa, on the other hand, showed an increased cell size in mixed cultures with O. marsonii. However, with respect to biomass production, M. aeruginosa did not profit from the growth inhibition of O. marsonii in the experimental period of 8 days. By contrast, the co‐cultivation of M. aeruginosa with S. obliquus did not affect the physiological activity of either species. This study highlights the potential of flow cytometry to determine species‐specific basic growth parameters, light‐use efficiency and biomass gain during co‐cultivation of different algal strains. It is further demonstrated that sorted cells can be used to analyse their macromolecular composition. The results of this study reveal very different species‐specific patterns of growth kinetics and light‐use‐efficiency of green algae in co‐cultivation experiments with a cyanobacterium.

show abstract

Enzymatic cell disruption of microalgae biomass in biorefinery processes

Demuez

Mahdy

Tomás‐Pejó

et al. 2015

Biotech & Bioengineering

142

View full text Add to dashboard Cite

When employing biotechnological processes for the procurement of biofuels and bio-products from microalgae, one of the most critical steps affecting economy and yields is the "cell disruption" stage. Currently, enzymatic cell disruption has delivered effective and cost competitive results when compared to mechanical and chemical cell disruption methods. However, the introduction of enzymes implies additional associated cost within the overall process. In order to reduce this cost, autolysis of microalgae is proposed as alternative enzymatic cell disruption method. This review aims to provide the state of the art of enzymatic cell disruption treatments employed in biorefinery processes and highlights the use of endopeptidases. During the enzymatic processes of microalgae life cycle, some lytic enzymes involved in cell division and programmed cell death have been proven useful in performing cell lysis. In this context, the role of endopeptidases is emphasized. Mirroring these natural events, an alternative cell disruption approach is proposed and described with the potential to induce the autolysis process using intrinsic cell enzymes. Integrating induced autolysis within biofuel production processes offers a promising approach to reduce overall global costs and energetic input associated with those of current cell disruption methods. A number of options for further inquiry are also discussed.

show abstract

Differences in staining of the unicellular algae Chlorococcales as a function of algaenan content

Cited by 12 publications

References 7 publications

Cell Wall Structure of Coccoid Green Algae as an Important Trade-Off Between Biotic Interference Mechanisms and Multidimensional Cell Growth

Cell Wall Structure of Coccoid Green Algae as an Important Trade-Off Between Biotic Interference Mechanisms and Multidimensional Cell Growth

Contrasting effects of the cyanobacterium Microcystis aeruginosa on the growth and physiology of two green algae, Oocystis marsonii and Scenedesmus obliquus, revealed by flow cytometry

Enzymatic cell disruption of microalgae biomass in biorefinery processes

Contact Info

Product

Resources

About