Differences in soil physicochemical properties and rhizosphere microbial communities of flue-cured tobacco at different transplantation stages and locations

Abstract

Rhizosphere microbiota play an important role in regulating soil physical and chemical properties and improving crop production performance. This study analyzed the relationship between the diversity of rhizosphere microbiota and the yield and quality of flue-cured tobacco at different transplant times (D30 group, D60 group and D90 group) and in different regions [Linxiang Boshang (BS) and Linxiang ZhangDuo (ZD)] by high-throughput sequencing technology. The results showed that there were significant differences in the physicochemical properties and rhizosphere microbiota of flue-cured tobacco rhizosphere soil at different transplanting times, and that the relative abundance of Bacillus in the rhizosphere microbiota of the D60 group was significantly increased. RDA and Pearson correlation analysis showed that Bacillus, Streptomyces and Sphingomonas were significantly correlated with soil physical and chemical properties. PIGRUSt2 function prediction results showed that compared with the D30 group, the D60 group had significantly increased metabolic pathways such as the superpathway of pyrimidine deoxyribonucleoside salvage, allantoin degradation to glyoxylate III and pyrimidine deoxyribonucleotides de novo biosynthesis III metabolic pathways. The D90 group had significantly increased metabolic pathways such as ubiquitol-8 biosynthesis (prokaryotic), ubiquitol-7 biosynthesis (prokaryotic) and ubiquitol-10 biosynthesis (prokaryotic) compared with the D60 group. In addition, the yield and quality of flue-cured tobacco in the BS region were significantly higher than those in the ZD region, and the relative abundance of Firmicutes and Bacillus in the rhizosphere microbiota of flue-cured tobacco in the BS region at the D60 transplant stage was significantly higher than that in the ZD region. In addition, the results of the hierarchical sample metabolic pathway abundance map showed that the PWY-6572 metabolic pathway was mainly realized by Paenibacillus, and that the relative abundance of flue-cured tobacco rhizosphere microbiota (Paenibacillus) participating in PWY-6572 in the D60 transplant period in the BS region was significantly higher than that in the ZD region. In conclusion, different transplanting periods of flue-cured tobacco have important effects on soil physical and chemical properties and rhizosphere microbial communities. There were significant differences in the rhizosphere microbiota and function of flue-cured tobacco in different regions, which may affect the performance and quality of this type of tobacco.

Effects of aftercrop tomato and maize on the soil microenvironment and microbial diversity in a long-term cotton continuous cropping field

“…The soil physicochemical properties, an important component of soil health, affect moisture, aeration, soil temperature, nutrient cycling, and root growth, which, in turn, affects crop yields and environmental quality ( Zhou et al, 2014 ; Sainju et al, 2022 ; Zhao and Nian, 2023 ). Crop rotations can have an impact on the soil physicochemical properties ( Sandhu et al, 2017 ), and long-term continuous cropping patterns can lead to imbalances in the soil nutrients ( Munishi et al, 2021 ).…”

Integrated metabolomic and metagenomic strategies shed light on interactions among planting environments, rhizosphere microbiota, and metabolites of tobacco in Yunnan, China

“… Zhou et al (2023) studied different intercropping system-modulated soil–bacterial communities that shaped the growth and development status of tobacco. Zhao et al (2023) studied the correlations between differences in soil physicochemical properties and diversified rhizosphere microbiota during different transplanting stages of tobacco at two sites of Linxiang district, Lincang city, Yunnan. Currently, there is no report on direct interactions between tobacco metabolites and its rhizosphere microbiota; thus, this study uses the major tobacco variety of Yunnan “Yun87” as the test cultivar in four experimental sites (i.e., Chuxiong City (CX) in Chuxiong Yi Autonomous Prefecture, Jianshui County (JS) in Honghe Hani Yi Autonomous Prefecture, Xiangyun County (XY) in Dali Bai Autonomous Prefecture, and Zhanyi District (ZY) in Qujing City), which are typical representative sites of tobacco-producing areas in Yunnan to conduct this experiment.…”

Interventions Change Soil Functions and the Mechanisms Controlling the Structure of Soil Microbial Communities

“…The lack of information regarding physicochemical soil environments is reported as one of the major problems. Soil physicochemical properties play a crucial role in determining soil microbiome composition and function [ 12 ]. However, most soil microbiome studies either do not include soil physicochemical properties or limit measurements to pH, soil organic carbon or total carbon, and total nitrogen.…”