Differences in serum protein 2D gel electrophoresis patterns of Przewalski's (Mongolian wild horse) and thoroughbred horses

Abstract

The objective of this study was to assess differences in serum protein expression profiles of Przewalski's (Mongolian wild horse) and thoroughbred horses using proteome analysis. The serum proteins were separated by two-dimensional electrophoresis (2-DE) and five different gene products were identified. Proteins represented by the five spots were identified by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS)/MS technology. The identities of all proteins were deduced based on their similarity to proteins in the human plasma protein database. Three proteins (a haptoglobin-2 alpha glycoprotein and two haptoglobin-2beta glycoproteins with different accession numbers) were downregulated in Przewalski's horse sera compared to thoroughbred horse sera. Moreover, two proteins (tetraspanin-18 and pM5) were upregulated in Przewalski's horses compared to thoroughbred horses. Haptoglobin-2 alpha and haptoglobin-2beta may serve as candidate molecules in future studies of inflammation, coagulation, immune modulation and pro-oxidant and antioxidant activity with consequential effects on the entire metabolism of the horse.

Analysis of genetic diversity and structure of Mongolian horse using microsatellite markers

“…In modern times, their use has diversified into areas such as leisure and tourism. Mongolian horses are one of the oldest breeds in the world [ 1 ], and are under a strong natural selection from the environment as they are typically not stabled or given supplemental feed [ 2 ].…”

Quantitative analysis of the serum proteome during early pregnancy in mares

“…Compared to previous studies that evaluated either a single protein or a small set of proteins, the application of proteomics allows large-scale identification of blood proteins. The normal serum/plasma proteome patterns of horses have been reported (Lepczy nski et al, 2018;Miller et al, 2004), and changes in plasma protein levels after prolonged physical exercise (Scoppetta et al, 2012) as well as differences in serum protein expression profiles between distinct horse breeds (Barsuren et al, 2015) have been identified through proteome analysis. Furthermore, there is growing interest in early-pregnancy-related changes in the serum/plasma proteome of domestic animals.…”

Label-Free Mass Spectrometry-Based Quantitative Proteomics Analysis of Serum Proteins During Early Pregnancy in Jennies (Equus asinus)

“…Thus far, several proteomics approaches have been widely and effectively applied to explore the blood proteome of equids. Two-dimensional (2D) gel electrophoresis is routinely used for assessing quantitative changes in serum and/or plasma proteins of horses, such as for establishing the serum protein map (9,10), evaluating changes in plasma proteins after prolonged physical exercise (11), and comparing differences in serum protein expression profiles between distinct breeds (12). Henze et al (13) evaluated genetic differences in the serum proteome of horses, donkeys, and mules using surface-enhanced laser desorption-ionization timeof-flight mass spectrometry (MS), and they found considerable differences in the proteome of horses and donkeys and that the proteome of mules showed a higher similarity to donkeys than to that of horses.…”