Differences in proteomic profile between two haemocyte types, granulocytes and hyalinocytes, of the flat oyster Ostrea edulis

Ballina, Nuria R. de la; Villalba, António; Cao, Asunción

doi:10.1016/j.fsi.2020.03.033

Cited by 12 publications

(10 citation statements)

References 133 publications

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“…Initially, the distinction between hyalinocytes and granulocytes was made based on morphological parameters, years later it has been confirmed that there are also functional differences between both haemolymph cell types after analysis of metabolic and functional parameters (122,221). Recently, research efforts are focusing on revealing dissimilar immune functions among marine invertebrate haemocyte subpopulations, for example in ascidians (222); crustaceans as shrimps (223)(224)(225)(226)(227)(228), lobsters (229) and crabs (230); gastropod molluscs as sea snails (231)(232)(233)(234), abalones (235) and sea hares (236); and bivalve molluscs as oysters (182,216,237,238). This review focuses on the major findings in functional differences between the main bivalve haemocyte types.…”

Section: Functional Differences Between Hyalinocytes and Granulocytesmentioning

confidence: 99%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

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Bivalve molluscs stand out for their ecological success and their key role in the functioning of aquatic ecosystems, while also constituting a very valuable commercial resource. Both ecological success and production of bivalves depend on their effective immune defence function, in which haemocytes play a central role acting as both the undertaker of the cellular immunity and supplier of the humoral immunity. Bivalves have different types of haemocytes, which perform different functions. Hence, identification of cell subpopulations and their functional characterisation in immune responses is essential to fully understand the immune system in bivalves. Nowadays, there is not a unified nomenclature that applies to all bivalves. Characterisation of bivalve haemocyte subpopulations is often combined with 1) other multiple parameter assays to determine differences between cell types in immune-related physiological activities, such as phagocytosis, oxidative stress and apoptosis; and 2) immune response to different stressors such as pathogens, temperature, acidification and pollution. This review summarises the major and most recent findings in classification and functional characterisation of the main haemocyte types of bivalve molluscs.

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Section: Functional Differences Between Hyalinocytes and Granulocytesmentioning

confidence: 99%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

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“…Hyaline amoebocyte consists of a few cytoplasmic granules, a central nucleus, (Adamowicz, 2005), and short pseudopodia. This cell was reported to be involved in wound healing and clotting activities (Ballina et al, 2020). Granular amoebocytes contain dense cytoplasmic granules (Kurek et al, 2007) and may represent a functional analogy to vertebrate neutrophils.…”

Section: Discussionmentioning

confidence: 99%

“…In bivalves, granular amoebocytes have been reported to play a pivotal role in phagocytic activity due to their large pseudopodia and high lysosomal contents (Cajaraville et al, 1995). They generate higher amounts of cytotoxic molecules as compared to hyaline amoebocytes (Ballina et al, 2020). Eleocytes play a major role in cellular metabolism and the production of bioactive molecules (Engelmann, Hayashi, Bodó et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

A comparative account of coelomocyte of earthworm ecotypes with reference to its morphology, morphometry, density, phagocytosis, autofluorescence, and oxidative status

Manna

Ray

Gautam

et al. 2022

Journal of Morphology

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Earthworms inhabit different strata of moist soil. Epigeic and endogeic earthworms prefer superficial and inner stratum of soil respectively, whereas, semiaquatic species are distributed around hydrated soil near ponds and lakes. Coelomocytes, the chief immunoeffector cells of coelomic origin, perform diverse physiological functions like phagocytosis, maintenance of cellular homeostasis, and acid–base balance of coelomic fluid, graft rejection, elicitation of cytotoxic, and oxidative responses under the challenges of pathogens and toxins. The present study aims to analyze selected morphological and functional parameters in three differentially adapted Indian earthworms of nonsimilar habitats. Coelomocytes of Glyphidrilus tuberosus (Stephenson, 1916) (semiaquatic), Perionyx excavatus (Perrier, 1872) (epigeic), and Eutyphoeus orientalis (Beddard, 1883) (endogeic) were isolated for morphological and morphometric analyses and subjected to determination of phagocytic, oxidative, and cytotoxic responses. Activities of phenoloxidase, pro, and antioxidant enzymes, and autofluorescence were determined in the extruded coelomocytes of earthworms of three contrasting habitats. The differential result may be correlated with species‐specific responses and variation in habitat preference and related adaptation.

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“…Sel hyalin adalah sel paling banyak ditemukan pada hemosit gonggong, L. turturella, yaitu berkisar antara 58%-80% dari jumlah keseluruhan sel, sedangkan sel granular adalah yang paling sedikit ditemukan, yaitu berkisar antara 20%-42%. Beberapa penelitian tentang hemolim pada hewan-hewan vertebrata, jumlah sel hyalin yang dominan pada hemosit kemudian diikuti dengan sel granular (Estrada et al, 2013;Hong et al, 2013;Ballina et al, 2020). Sel hyalin melakukan fungsi dalam imunitas sebagai fagositosis (Thornqvist et al, 1994).…”

Section: Kampung Madong Lobamunclassified

“…Sel granular ini juga berkorelasi dengan kapasitasnya dalam intracellular killing. Ballina et al (2020) menyatakan bahwa sel granular dipercaya lebih efisien dalam membunuh mikroorganisme, sedangkan sel hyaline lebih spesifik dalam penggumpalan dan penyembuhan luka.…”

Section: Kampung Madong Lobamunclassified

PROFIL HEMOLIM SIPUT GONGGONG, Laevistrombus turturella ASAL PERAIRAN PESISIR PULAU BINTAN PROVINSI KEPULAUAN RIAU SEBAGAI KANDIDAT BIOTA BUDIDAYA

Muzahar

Zahra

Wulandari

2022

Jurnal Riset Akuakultur

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Siput gonggong, Laevistrombus turturella termasuk komoditas perikanan laut yang memiliki nilai ekonomi tinggi di Pulau Bintan Provinsi Kepulauan Riau. Hemolim pada siput berperan penting dalam sistem pertahanan tubuh dan reproduksi. Penelitian ini bertujuan untuk mengetahui total hemosit dan diferensial hemosit pada siput gonggong asal Pulau Bintan. Penelitian dilaksanakan pada bulan Juli-Agustus 2021. Sampel siput gonggong berasal dari perairan laut Kampung Madong dan Lobam. Hemolim diambil dari otot kaki gonggong untuk pemeriksaan total hemosit dan diferensial hemosit. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan total hemosit, persentase hyalin dan sel granular siput gonggong dari Kampung Madong dan Lobam relatif sama dan dalam kisaran normal (< 1,0 x 106 sel/mm3). Jumlah total hemosit siput gonggong asal Kampung Madong dan Lobam secara berturut-turut adalah berkisar antara 77.000-166.600 sel/mm3 dan 71.000-165.000 sel/mm3, persentase sel hyalin berkisar antara, 58%-80% dan 52%-70%, serta persentase sel granular berkisar antara 20%-42% dan 30%-48%. Oleh karena nilai total hemosit dan diferensial hemosit relatif sama maka siput gonggong asal Kampung Madong disarankan sebagai kandidat calon induk karena memiliki ukuran tubuh 6,52 ± 5,61 cm lebih besar dibandingkan asal Lobam 5,27 ± 0,40 cm.Gonggong conch, Laevistrombus turturella is a marine gastropod highly valued as a seafood commodity in Bintan Island Riau Islands Province. The farming technology of the species is currently not available due to limited information on its biology including its immune system and reproduction. Hemolymph in conchs plays an important role in gonggong conch body’s defense system and reproduction. This study aimed to determine the total haemocytes and hemocytic differential of gonggong conchs originated from Bintan Island. The research was conducted between July-August 2021. The gonggong conch samples were collected from the coastal waters of Kampung Madong and Lobam. Hemolymph was taken from the gonggong conch leg muscles for examination of total hemocytes and hemocytic differential and analyzed descriptively. The results showed that the total hemocytes, percentage of hyaline and granular cells of gonggong conchs from Kampung Madong and Lobam were relatively similar and within the normal range (< 1.0 x106 cell/mm3). The total number of haemocytes of gonggong conchs from Kampung Madong and Lobam ranged from 77,000-166,600 cells/mm3and 71,000-165,000 cells/mm3; respectively, while the percentages of hyaline cells ranged between 58%-80% and 52%-70%, respectively, and the percentage of granular cells ranged between 20%-42% and 30%-48%. Gongong conch from Kampung Madong had a body size of 6.52 ± 5.61 cm which was larger than that of Lobam with body size of 5.27 ± 0.40 cm. Because both had relatively similar values of total haemocytic and haemocytic differential, the gonggong conch from Kampung Madong was suggested as the best candidate for broodstock.

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Differences in proteomic profile between two haemocyte types, granulocytes and hyalinocytes, of the flat oyster Ostrea edulis

Cited by 12 publications

References 133 publications

Bivalve Haemocyte Subpopulations: A Review

Bivalve Haemocyte Subpopulations: A Review

A comparative account of coelomocyte of earthworm ecotypes with reference to its morphology, morphometry, density, phagocytosis, autofluorescence, and oxidative status

PROFIL HEMOLIM SIPUT GONGGONG, Laevistrombus turturella ASAL PERAIRAN PESISIR PULAU BINTAN PROVINSI KEPULAUAN RIAU SEBAGAI KANDIDAT BIOTA BUDIDAYA

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