Differences in production of several extracellular virulence factors in clinical and food <i>Aeromonas</i> spp. strains

Pin, Carmen; Marín, María; Selgas, Dolores; García, María L.; Tormo, J.; Casás, Carmen

doi:10.1111/j.1365-2672.1995.tb02839.x

Cited by 20 publications

(13 citation statements)

References 23 publications

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“…Production of exotoxin (a and b-hemolysin) is associated with enteric disturbances, as reported by Burke et al (1984b), Daily et al (1981, Handfield et al (1996), Macedo et al (1997), Matté (1995, Monfort & Baleux (1991b), Pin et al (1995), Singh and Sanyal (1992a, b). These results highlight the pathogenic potential of Aeromonas species and pose a public health concern.…”

Section: Resultsmentioning

confidence: 71%

“…These organisms are well recognized emerging pathogens responsible for gastrointestinal disturbances (Havelaar et al 1987;Joseph et al 1991;Monfort & Baleux 1991a;Singh & Sanyal 1992a,b;Hä ninen et al 1995;Villarruel-Ló pez et al 2005), septicemia, endocarditis, conjunctivitis and wound infections (Freitas et al 1993;Merino et al 1995;Pin et al 1995;Figueras et al 2005;Hiransuthikul et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains

Razzolini

Bari²,

Sanchez

et al. 2007

Journal of Water and Health

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Aeromonads are inhabitants of aquatic ecosystems and are described as being involved in intestinal disturbances and other infections. A total of 200 drinking water samples from domestic and public reservoirs and drinking fountains located in Sã o Paulo (Brazil), were analyzed for the presence of Aeromonas. Samples were concentrated by membrane filtration and enriched in APW. ADA medium was used for Aeromonas isolation and colonies were confirmed by biochemical characterization. Strains isolated were tested for hemolysin and toxin production.Aeromonas was detected in 12 samples (6.0%). Aeromonas strains (96)

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Section: Resultsmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains

Razzolini

Bari²,

Sanchez

et al. 2007

Journal of Water and Health

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“…infection in humans (8,20,21,22,24,29,34). Therefore, one of the purposes of the study was to investigate whether the same clones exist in the environment and in the stool specimens collected from patients with diarrhea.…”

Section: Discussionmentioning

confidence: 99%

Typing of Clinical and Environmental Aeromonas sp. Strains by Random Amplified Polymorphic DNA PCR, Repetitive Extragenic Palindromic PCR, and Enterobacterial Repetitive Intergenic Consensus Sequence PCR

Szczuka

Kaznowski

2004

J Clin Microbiol

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A collection of 120 strains isolated from stool specimens collected from humans suffering from gastroenteritis and from environmental samples were analyzed by random amplified polymorphic DNA PCR (RAPD), repetitive extragenic palindromic PCR (REP-PCR), and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR). Species of Aeromonoas hydrophila, A. bestiarum, A. salmonicida, A. caviae, A. media, and A. veronii revealed clonal structure. There was no dominant clone causing gastroenteritis in humans. Moreover, there was no genetic similarity between clinical and environmental strains of Aeromonas sp. isolated from different geographical areas as well as from the same geographical area. Some clones colonized specific ecosystems, e.g., drinking water distribution systems. RAPD and ERIC-PCR methods had the same discriminatory power and proved to be useful for epidemiological investigation and population genetic analysis of Aeromonas spp., whereas REP-PCR was less effective for differentiating the isolates of Aeromonas spp.Bacteria of Aeromonas sp. are gram-negative, straight cells (rod-shaped to coccoid) with rounded ends. They are oxidase and catalase positive, reduce nitrate to nitrite, and ferment D-glucose. These bacteria are widely spread in the environment, especially in surface water and sewage; they also occur in untreated and treated drinking water (1, 2, 4, 18). In humans, Aeromonas spp. are responsible for gastroenteritis, chronic diarrhea, wound infections, respiratory tract infections, peritonitis, urinary tract infections, and septicemia (2, 17). Among Aeromonas-associated infections of humans A. hydrophila, A. caviae, and A. veronii are the predominating species, whereas A. eucrenophila, A. popoffii, (2), and A. culicicola (28) have never been found in clinical samples. Some Aeromonas species are associated with a wide variety of diseases in cold-and warm-blooded animals, including fish, frogs, water buffaloes, reptiles, birds, and cattle (16, 28). A. veronii, A. hydrophila, and A. salmonicida are capable of causing septicemia in freshwater and marine fish (3, 9). Clinical and environmental Aeromonas sp. isolates secrete many extracellular products, such as hemolysins, enterotoxins, and proteases. Studies conducted by Kühn et al. (22) showed that some isolates of a given species produce virulence factors more frequently than others. These findings indicate that the virulence within the genus Aeromonas might be a clonal property and only some clones may be responsible for progressive disease. However, there have been no studies that would have determined clonal structure within Aeromonas spp. and the spread of specific clones in human population and in the environment. Our study was undertaken to recognize the clonal relatedness of strains derived from diarrheal stool specimens collected from humans living in different geographical areas. We also compared genetic similarities of clones recovered from stool and the environment. In addition, we attempted to determine the genetic relati...

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“…Aeromonas genus is considered an emerging pathogen (26,27) and responsible for gastrointestinal disturbances (19), septicemia, endocarditis, conjunctivitis and wound infections (8,9,13,16,18). These organisms are largely distributed in several water sources such as rivers, lakes, wells, sewage, wastewaters and drinking water supplies (19,24).…”

Section: Introductionmentioning

confidence: 99%

Aeromonas presence in drinking water from collective reservoirs and wells in peri-urban area in Brazil

Razzolini¹,

Günther²,

Martone-Rocha³

et al. 2010

Braz. J. Microbiol.

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Aeromonas genus is considered an emerging pathogen and its presence in drinking water supplies is a reason to public health concern. This study investigated the occurrence of Aeromonas in samples from collective reservoirs and wells used as drinking water sources in a peri-urban area. A total of 35 water samples were collected from collective reservoirs and 32 from wells bimonthly, from September 2007 to September 2008. Aeromonas spp determination was carried out using a Multiple-Tube Technique. Samples were inoculated into alkaline peptone water and the superficial film formed was transferred to blood agar plates amended with ampicillin. Typical Aeromonas colonies were submitted to a biochemical screening and then to biochemical tests for species differentiation. Aeromonas was detected in 13 (19%) of the 69 samples examined (6 from collective reservoirs and 7 from wells). Concentrations of Aeromonas in collective reservoirs ranged from <0.3 to 1.2 x10 2 MPN/100mL and, in wells, from <0.3 to 2.4 x10 2 MPN/100mL. The most frequent specie in the collective reservoir samples was Aeromonas spp (68%), followed by A. encheleia (14%) and A. allosaccharophila (8%) and A. hydrophila (8%). Aeromonas spp (87%) was the most frequent specie isolated from well samples, followed by A. allosacchariphila (8%), A. encheleia (2%) and A. jandaei (5%). These data show the presence and diversity of Aeromonas genus in the samples analyzed and highlight that its presence in drinking water poses a significant public health concern.

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Differences in production of several extracellular virulence factors in clinical and food Aeromonas spp. strains

Cited by 20 publications

References 23 publications

Aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains

Aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains

Typing of Clinical and Environmental Aeromonas sp. Strains by Random Amplified Polymorphic DNA PCR, Repetitive Extragenic Palindromic PCR, and Enterobacterial Repetitive Intergenic Consensus Sequence PCR

Aeromonas presence in drinking water from collective reservoirs and wells in peri-urban area in Brazil

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