Differences in monocarboxylic acid transporter type 1 expression in rumen epithelium of newborn calves due to age and milk or milk replacer feeding

Flaga, Jadwiga; Górka, P.; Zabielski, R.; Kowalski, Z.M.

doi:10.1111/jpn.12218

Cited by 12 publications

(11 citation statements)

References 41 publications

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“…This result is similar to a previous study for the MCT1 short-chain fatty acid transporter, whose abundance did not differ between dorsal and ventral sacs in developing calves (Flaga et al 2015). Since we have previously reported that UT-B2 transporters are constitutively activated (Tickle et al 2009), this strongly suggests that overall there will be no difference in UT-B mediated trans-epithelial transport in different ruminal sacs at the cellular level.…”

Section: Discussionsupporting

confidence: 91%

UT-B Urea Transporter Localization in the Bovine Gastrointestinal Tract

et al. 2015

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Facilitative UT-B urea transporters play an important role in the urea nitrogen salvaging process that occurs in the gastrointestinal tract of mammals, particularly ruminants. Gastrointestinal UT-B transporters have previously been reported in various ruminant species-including cow, sheep and goat. In this present study, UT-B transporter localization was investigated in tissues throughout the bovine gastrointestinal tract. RT-PCR analysis showed that UT-B2 was the predominant UT-B mRNA transcript expressed in dorsal, ventral and cranial ruminal sacs, while alternative UT-B transcripts were present in other gastrointestinal tissues. Immunoblotting analysis detected a strong, glycosylated ~50 kDa UT-B2 protein in all three ruminal sacs. Immunolocalization studies showed that UT-B2 protein was predominantly localized to the plasma membrane of cells in the stratum basale layer of all ruminal sac papillae. In contrast, other UT-B protein staining was detected in the basolateral membranes of the surface epithelial cells lining the abomasum, colon and rectum. Overall, these findings confirm that UT-B2 cellular localization is similar in all ruminal sacs and that other UT-B proteins are located in epithelial cells lining various tissues in the bovine gastrointestinal tract.

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Section: Discussionsupporting

confidence: 91%

UT-B Urea Transporter Localization in the Bovine Gastrointestinal Tract

et al. 2015

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“…These factors also trigger activation of downstream targets that can mediate rumen development and function in growing calves12. Changes of monocarboxylate transporter 1 ( MCT1 ) expression occur in an age-dependent manner in the rumen epithelium of neonatal calves, which suggests that the expression of MCT1 may be affected by the provision of liquid feed13. During the studies described above, many changes in gene expression related to rumen development were identified in calves.…”

mentioning

confidence: 99%

Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs

Wang

et al. 2016

Sci Rep

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Early consumption of starter feed promotes rumen development in lambs. We examined rumen development in lambs fed starter feed for 5 weeks using histological and biochemical analyses and by performing high-throughput sequencing in rumen tissues. Additionally, rumen contents of starter feed-fed lambs were compared to those of breast milk-fed controls. Our physiological and biochemical findings revealed that early starter consumption facilitated rumen development, changed the pattern of ruminal fermentation, and increased the amylase and carboxymethylcellulase activities of rumen micro-organisms. RNA-seq analysis revealed 225 differentially expressed genes between the rumens of breast milk- and starter feed-fed lambs. These DEGs were involved in many metabolic pathways, particularly lipid and carbohydrate metabolism, and included HMGCL and HMGCS2. Sequencing analysis of 16S rRNA genes revealed that ruminal bacterial communities were more diverse in breast milk-than in starter feed-fed lambs, and each group had a distinct microbiota. We conclude that early starter feeding is beneficial to rumen development and physiological function in lambs. The underlying mechanism may involve the stimulation of ruminal ketogenesis and butanoate metabolism via HMGCL and HMGCS2 combined with changes in the fermentation type induced by ruminal microbiota. Overall, this study provides insights into the molecular mechanisms of rumen development in sheep.

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“…A recent study reported that gene expression related to rumen development shows dynamic change between preweaning and postweaning calves (Kato et al, 2016). Flaga et al (2015) showed that age-dependent increase in MCT1 gene expression was detected in the rumen of calves before weaning (Flaga et al, 2015). Age-dependent changes of MCT4 and CD147, however, have not been investigated in calves even if those are significant for absorption of SCFAs.…”

Section: Introductionmentioning

confidence: 99%

Propionate and butyrate induce gene expression of monocarboxylate transporter 4 and cluster of differentiation 147 in cultured rumen epithelial cells derived from preweaning dairy calves1

Nakamura

Haga

Kimura

et al. 2018

Journal of Animal Science

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Short-chain fatty acids (SCFAs) are the main source of energy for postweaning ruminants. The monocarboxylic acid transporters, MCT1 and MCT4, are thought to contribute to the absorption of SCFAs from the surface of the rumen following weaning. The present study measured changes in MCT1 and MCT4 expression in ruminal epithelial cells isolated from male preweaning (22 to 34 d old, n = 6) and postweaning (55 to 58 d old, n = 8) calves after euthanasia and sought to examine whether SCFAs stimulate the expression of these transporters. In the current study, cluster of differentiation 147 (CD147) gene expression in the rumen was also investigated since CD147 has been considered to act as ancillary protein for MCT1 and MCT4 to express their correct function. The gene expression levels of MCT1, MCT4, and CD147 in the rumen were found to be significantly higher in postweaning calves than in preweaning calves. Strong MCT1 immunoreactivity was detected in both the stratum basale (SB) and the stratum spinosum (SS) in postweaning ruminal epithelium. Expression of MCT1 in preweaning calves was localized to a specific region of the SB and of the SS. MCT4-immunopositive cells were detected in the stratum corneum (SC) of the ruminal epithelium in postweaning calves. However, only a low level of signal was detected in the SC of preweaning animals. Furthermore, in vitro experiments, ruminal epithelial cells were incubated for 24 h with acetate (0.04, 0.4, and 4 mM), propionate (0.2, 2, and 20 mM), butyrate (0.1, 1, and 10 mM), or β-hydroxybutyrate (BHBA; 0.1, 1, and 10 mM), respectively. Both propionate and butyrate induced an increase in the gene expression levels of MCT4 and CD147, but did not affect MCT1 gene expression. There are no significant effects of acetate and BHBA treatment on these gene expressions. Taken together, these results suggest that an increase in MCT4 and CD147 gene expression in the ruminal epithelium of postweaning calves is likely to be due to the effects of propionate and butyrate derived from a solid-based diet, which may contribute to ruminal development following weaning.

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Differences in monocarboxylic acid transporter type 1 expression in rumen epithelium of newborn calves due to age and milk or milk replacer feeding

Cited by 12 publications

References 41 publications

UT-B Urea Transporter Localization in the Bovine Gastrointestinal Tract

UT-B Urea Transporter Localization in the Bovine Gastrointestinal Tract

Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs

Propionate and butyrate induce gene expression of monocarboxylate transporter 4 and cluster of differentiation 147 in cultured rumen epithelial cells derived from preweaning dairy calves1

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