Differences in metabolites production using the Biolog FF Microplate™ system with an emphasis on some organic acids of Aspergillus niger wild type strains

Šimonovičová, Alexandra; Kupka, Daniel; Nosalj, Sanja; Kraková, Lucia; Drahovská, Hana; Bártová, Zuzana; Vojtková, Hana; Boturová, Kateřina; Pangallo, Domenico

doi:10.2478/s11756-020-00521-y

Cited by 14 publications

(6 citation statements)

References 55 publications

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“…The Biolog technique was also used in ecological studies to estimate the metabolic potential of microbial communities from rhizospheric soil ( Stefanowicz, 2006 ), substrate utilisation, growth, secondary metabolite, and the antimicrobial profiles of some fungal cultures important for the microbial drug discovery program ( Singh, 2009 ). The Biolog system was used to enable the evaluation of the metabolic profile diversity of microbial populations in environmental samples, which reflects the state of their activity ( Gryta et al, 2014 ; Šimonovičová et al, 2020 ). Twelve Aspergillus sp.…”

Section: Introductionmentioning

confidence: 99%

Aspergillus niger Environmental Isolates and Their Specific Diversity Through Metabolite Profiling

et al. 2021

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We present a biological profile of 16 Aspergillus niger environmental isolates from different types of soils and solid substrates across a pH range, from an ultra-acidic (<3.5) to a very strongly alkaline (>9.0) environment. The soils and solid substrates also differ in varying degrees of anthropic pollution, which in most cases is caused by several centuries of mining activity at old mining sites, sludge beds, ore deposits, stream sediments, and coal dust. The values of toxic elements (As, Sb, Zn, Cu, Pb) very often exceed the limit values. The isolates possess different macro- and micromorphological features. All the identifications of Aspergillus niger isolates were confirmed by molecular PCR analysis and their similarity was expressed by RAMP analysis. The biochemical profile of isolates based on FF-MicroPlate tests from the Biolog system showed identical biochemical reactions in 50 tests, while in 46 tests the utilisation reactions differed. The highest similarity of strains isolated from substrates with the same pH, as well as the most suitable biochemical tests for analysis of the phenotypic similarity of isolated strains, were confirmed when evaluating the biochemical profile using multicriterial analysis in the Canoco program. The isolates were screened for mycotoxin production by thin-layer chromatography (TLC), as well. Two of them were able to synthesise ochratoxin A, while none produced fumonisins under experimental conditions. Presence of toxic compounds in contaminated sites may affect environmental microscopic fungi and cause the genome alteration, which may result in changes of their physiology, including the production of different (secondary) metabolites, such as mycotoxins.

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Section: Introductionmentioning

confidence: 99%

Aspergillus niger Environmental Isolates and Their Specific Diversity Through Metabolite Profiling

et al. 2021

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“…The growth of moss as a pioneer plant is pointed out by a number of works dealing with the biological diversity of anthropogenically polluted places, especially after the mining and processing of ores [9][10][11][12]. The results are significant in terms of studying the adaptation of living organisms to anthropogenic influences, mainly due to bioremediation of soils [13][14][15].…”

Section: Resultsmentioning

confidence: 99%

Biomonitoring of Post-Mine Environment Using Ruderal Plants

KUČOVÁ¹

2022

GSE

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Ruderal plants that remove toxic metals by accumulating in their tissues are called accumulators. Such accumulators can be used for phytoremediation or phytomining. The use of plants for biomonitoring environmental pollution by a non-invasive monitoring method correlates the effects of anthropogenic factors and living organisms. The advantage is the early detection of changes in the environment, thanks to the rapid response of bioindicators, responding to changes in the habitat, for example, by creating deformations. At present, biomonitoring is one of the indispensable parts of nature and environmental protection. The following species were selected for research -Aster amellus L., Rosa canina L. and Paraleucobryum longifolium; the area of interest was the post-mining area. The aim of the study was to verify the hyperaccumulation activity of ruderal plants on the thermally active dump pile Ema, in the district of Ostrava-Karviná, Czech Republic. The plant samples were taken in the places with the thermal activity and were compared as for the capacity of bioaccumulation of hazardous metals -Co, Mo, Cd, Pb, Cr, As, Ba, Sr in the plant tissues from different parts of the plants (root, stem, leaf, fruit). The results were compared with the collection of metals at sampling points. The highest ability to bioaccumulate was demonstrated by plants from the Bryophyta division.

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“…In the exteriors, experiments were conducted under static conditions, and in this case, too, we recorded lower values of Ni content reduction in the aqueous medium (samples 9 and 10) compared with the anhydrous environment (samples 11 and 12). The species A. niger is a known producer of many organic acids and secondary metabolites ( Šimonovičová et al, 2020 , 2021 ) which are used in bioleaching processes. It can be assumed that in the case of adding distilled water, the organic acids produced were diluted, which caused a slowing down/reduction of the bioleaching process from the solid substrate.…”

Section: Resultsmentioning

confidence: 99%

Experimental Treatment of Hazardous Ash Waste by Microbial Consortium Aspergillus niger and Chlorella sp.: Decrease of the Ni Content and Identification of Adsorption Sites by Fourier-Transform Infrared Spectroscopy

et al. 2021

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Despite the negative impact on the environment, incineration is one of the most commonly used methods for dealing with waste. Besides emissions, the production of ash, which usually shows several negative properties, such as a higher content of hazardous elements or strongly alkaline pH, is problematic from an environmental viewpoint as well. The subject of our paper was the assessment of biosorption of Ni from ash material by a microbial consortium of Chlorella sp. and Aspergillus niger. The solid substrate represented a fraction of particles of size <0.63 mm with a Ni content of 417 mg kg–1. We used a biomass consisting of two different organisms as the sorbent: a non-living algae culture of Chlorella sp. (an autotrophic organism) and the microscopic filamentous fungus A. niger (a heterotrophic organism) in the form of pellets. The experiments were conducted under static conditions as well as with the use of shaker (170 rpm) with different modifications: solid substrate, Chlorella sp. and pellets of A. niger; solid substrate and pellets of A. niger. The humidity-temperature conditions were also changed. Sorption took place under dry and also wet conditions (with distilled water in a volume of 30–50 ml), partially under laboratory conditions at a temperature of 25°C as well as in the exterior. The determination of the Ni content was done using inductively coupled plasma optical emission spectrometry (ICP-OES). The removal of Ni ranged from 13.61% efficiency (Chlorella sp., A. niger with the addition of 30 ml of distilled water, outdoors under static conditions after 48 h of the experiment) to 46.28% (Chlorella sp., A. niger with the addition of 30 ml of distilled water, on a shaker under laboratory conditions after 48 h of the experiment). For the purpose of analyzing the representation of functional groups in the microbial biomass and studying their interaction with the ash material, we used Fourier-transform infrared (FTIR) spectroscopy. We observed that the amount of Ni adsorbed positively correlates with absorbance in the spectral bands where we detect the vibrations of several organic functional groups. These groups include hydroxyl, aliphatic, carbonyl, carboxyl and amide structural units. The observed correlations indicate that, aside from polar and negatively charged groups, aliphatic or aromatic structures may also be involved in sorption processes due to electrostatic attraction. The correlation between absorbance and the Ni content reached a maximum in amide II band (r = 0.9; P < 0.001), where vibrations of the C=O, C–N, and N–H groups are detected. The presented results suggest that the simultaneous use of both microorganisms in biosorption represents an effective method for reducing Ni content in a solid substrate, which may be useful as a partial process for waste disposal.

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Differences in metabolites production using the Biolog FF Microplate™ system with an emphasis on some organic acids of Aspergillus niger wild type strains

Cited by 14 publications

References 55 publications

Aspergillus niger Environmental Isolates and Their Specific Diversity Through Metabolite Profiling

Aspergillus niger Environmental Isolates and Their Specific Diversity Through Metabolite Profiling

Biomonitoring of Post-Mine Environment Using Ruderal Plants

Experimental Treatment of Hazardous Ash Waste by Microbial Consortium Aspergillus niger and Chlorella sp.: Decrease of the Ni Content and Identification of Adsorption Sites by Fourier-Transform Infrared Spectroscopy

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