Differences in metabolic parameters and gene expression related to Osteochondrosis/Osteoarthrosis in pigs fed 25-hydroxyvitamin D₃

Abstract

-Osteochondrosis/osteoarthrosis (OC/OA) are common terms for various joint pathologies that occur in pigs. Pathologies that may contribute to these disorders have been described, but the primary cause(s) remain unknown. We hypothesised that as OC has some similarities to dyschondroplasia, which involves a failure of growth plate chondrocytes to fully differentiate and hypertrophy, treatment with 25-hydroxyvitamin D 3 (25-D) might reduce the incidence and/or severity of lesions in pigs, as it does in chickens with dyschondroplasia. Control pigs were fed a commercial diet ad libitum. In the treated group this diet was supplemented with 25-D at 0.1mg/kg. Ten pigs from each of the control and treated groups were sampled at 7, 12, 16 and 21 weeks. Treatment with 25-D had no effect on the incidence or severity of OC/OA lesions. Cartilage dry weight, total collagen content and proteoglycan content, and plasma levels of total calcium, inorganic phosphorous, vitamin C, insuline-like growth factor-I, parathyroid hormone and tumour necrosis factor alpha were unaffected by treatment. In addition, none of these parameters were correlated with the incidence or severity of OC/OA lesions. The mRNA expression levels of 21 out of 23 genes assayed by RT-PCR were unaltered in articular cartilage from OA lesion samples as compared to normal articular cartilage. However, collagen type II was reduced and collagen type X increased in OA lesion and near lesion samples. These results suggest that OA in pigs may share some features of osteoarthritis in other mammalian species. Ostéo-chondrite et arthrose (OC/AR) sont des termes courants désignant les diverses pathologies des articulations survenant chez le porc. Les maladies pouvant contribuer à ces troubles ont été décrites, mais les causes premières demeurent inconnues. Nous avons fait l'hypothèse que, puisque l'ostéochon-drite a quelques similarités avec la dyschondroplasie, qui entraîne une incapacité de la plaque endochondriale à se différencier et à s'hypertrophier, le traitement par la 25-hydroxyvitamine D 3 (25-D) pourrait réduire l'incidence et/ou la sévérité des lésions chez le porc, comme elle le fait chez les poulets atteints de dyschondroplasie. Des porcs témoins ont été nourris à volonté par des rations commerciales. Dans le groupe des porcs traités, cette ration a été complétée par de la 25-D à 0,1 mg/kg. Dix porcs provenant de chaque groupe (témoin et traité) ont été examinés aux semaines 7, 12, 16 et 21. Le traitement par la 25-D n'a eu aucun effet sur l'incidence ou la sévérité des lésions OC/AR. Le poids sec des cartilages, le contenu total en collagène et en protéoglycanes, et les taux plasmatiques en calcium total, en phosphore inorganique, en vitamine C, en facteur de croissance proche de l'insuline-I (IGF-I), en hormone parathyroïdienne, et en facteur de nécrose des tumeurs-α (TNFα), n'ont pas été affectés par le traitement. De plus, aucun de ces paramètres n'était corrélé à l'incidence ou à la sévéri-té des lésions OC/AR. Les niveaux d'expression de l'ARNm...

Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring

“…However, col1a1 was upregulated in the menisci of the animals used in the presented study which was identified as degeneration of the meniscal tissue [45]; Thus a change in the assessed articular cartilage should have been detected. For other genes, for example acan , our findings were shared with some authors but differed from the findings of others [12,18,21,41,125,127,128,129]. The lack of difference in the gene expression results between the ACLR- and sham-operated groups in our study could be expected as it is in accordance with our histological findings.…”

Functional characterization of TRPV4 as an osmotically sensitive ion channel in porcine articular chondrocytes

“…RNA was extracted from porcine chondrocytes and reverse-transcribed using commercial kits (RNeasy Mini Kit; Qiagen, Valencia, CA, and SuperScript™ First-Strand Synthesis System for RT-PCR; Invitrogen, Carlsbad, CA). PCR was performed using reagents from Qiagen, and porcine GAPDH (33, 37) was used as a housekeeping gene. The PCR products were submitted to a core facility (Duke University DNA Analysis Facility, Durham, NC) for sequencing.…”

Role of Insulin-Transferrin-Selenium in Auricular Chondrocyte Proliferation and Engineered Cartilage Formation in Vitro

“…Primer sequences were quoted from references [27,28] or designed using Gene Runner software (version 3.05, Hastings Software Inc. Hastings, NY, USA). The specificity of sequences was verified using the basic local alignment search tool (BLAST) of the National Center for Biotechnology Information (NCBI) online database.…”