Differences in liver microRNA profiling in pigs with low and high
                    feed efficiency

Miao, Yuanxin; Fu, Chuanke; Liao, Mingxing; Fang, Fang

doi:10.5187/jast.2022.e4

Cited by 2 publications

(3 citation statements)

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“…Therefore, adipose tissue acts as an energy depot to mediate metabolic homeostasis and nutrient availability, thereby further regulating feed efficiency [ 50 ]. miRNAs can modulate various biological processes through modulating tissue that takes part in energy metabolism and is associated with pig feed efficiency [ 24 , 25 , 51 , 52 , 53 ]. In this study, the miRNA expression profiles of adipose tissues from high-FE and low-FE pigs were compared to reveal the miRNA-mediated regulation of FE.…”

Section: Discussionmentioning

confidence: 99%

“…A total of 15 DEMs were identified in skeletal muscle between high- and low-RFI pigs, and they mainly take part in skeletal muscle growth and development [ 24 ]. In addition, 14 DEMs have been found in the liver of pigs with significant RFI differences, and they are mainly related to the signaling pathways of insulin, GnRH and mTOR [ 25 ]. These results indicate that miRNAs are vital regulators of FE.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Differentially Expressed miRNAs in Porcine Adipose Tissues and Evaluation of Their Effects on Feed Efficiency

Liao

Ren

Miao

2022

Genes

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Feed efficiency (FE) is a very important trait affecting the economic benefits of pig breeding enterprises. Adipose tissue can modulate a variety of processes such as feed intake, energy metabolism and systemic physiological processes. However, the mechanism by which microRNAs (miRNAs) in adipose tissues regulate FE remains largely unknown. Therefore, this study aimed to screen potential miRNAs related to FE through miRNA sequencing. The miRNA profiles in porcine adipose tissues were obtained and 14 miRNAs were identified differentially expressed in adipose tissues of pigs with extreme differences in FE, of which 9 were down-regulated and 5 were up-regulated. GO and KEGG analyses indicated that these miRNAs were significantly related to lipid metabolism and these miRNAs modulated FE by regulating lipid metabolism. Subsequently, quantitative reverse transcription–polymerase chain reaction (qRT-PCR) of five randomly selected DEMs was used to verify the reliability of miRNA-seq data. Furthermore, 39 differentially expressed target genes of these DEMs were obtained, and DEMs–target mRNA interaction networks were constructed. In addition, the most significantly down-regulated miRNAs, ssc-miR-122-5p and ssc-miR-192, might be the key miRNAs for FE. Our results reveal the mechanism by which adipose miRNAs regulate feed efficiency in pigs. This study provides a theoretical basis for the further study of swine feed efficiency improvement.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Differentially Expressed miRNAs in Porcine Adipose Tissues and Evaluation of Their Effects on Feed Efficiency

Liao

Ren

Miao

2022

Genes

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“…The liver plays an essential, multifunctional role in mammals, including hematopoiesis in the embryonic and fetal stages and metabolism, immunity, and clotting in the postnatal stages [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. In particular, the liver is the animal metabolic hub [ 29 , 30 , 31 ] and closely associated with economic traits (e.g., meat yield, feed efficiency, and growth rate) in pigs [ 32 , 33 , 34 ]. Moreover, porcine liver is a potential source of xenotransplantation in humans [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Profiling of Chromatin Accessibility in Pigs across Multiple Tissues and Developmental Stages

Bai,

Lin,

Zhang

et al. 2023

IJMS

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The study of chromatin accessibility across tissues and developmental stages is essential for elucidating the transcriptional regulation of various phenotypes and biological processes. However, the chromatin accessibility profiles of multiple tissues in newborn pigs and across porcine liver development remain poorly investigated. Here, we used ATAC-seq and rRNA-depleted RNA-seq to profile open chromatin maps and transcriptional features of heart, kidney, liver, lung, skeletal muscle, and spleen in newborn pigs and porcine liver tissue in the suckling and adult stages, respectively. Specifically, by analyzing a union set of protein-coding genes (PCGs) and two types of transcripts (lncRNAs and TUCPs), we obtained a comprehensive annotation of consensus ATAC-seq peaks for each tissue and developmental stage. As expected, the PCGs with tissue-specific accessible promoters had active transcription and were relevant to tissue-specific functions. In addition, other non-coding tissue-specific peaks were involved in both physical activity and the morphogenesis of neonatal tissues. We also characterized stage-specific peaks and observed a close association between dynamic chromatin accessibility and hepatic function transition during liver postnatal development. Overall, this study expands our current understanding of epigenetic regulation in mammalian tissues and organ development, which can benefit both economic trait improvement and improve the biomedical usage of pigs.

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Differences in liver microRNA profiling in pigs with low and high feed efficiency

Abstract: Competing interestsNo potential conflict of interest relevant to this article was reported. Funding sourcesState funding sources (grants, funding sources, equipment, and supplies). Include name and number of grant if available.

Cited by 2 publications

References 88 publications

Identification of Differentially Expressed miRNAs in Porcine Adipose Tissues and Evaluation of Their Effects on Feed Efficiency

Identification of Differentially Expressed miRNAs in Porcine Adipose Tissues and Evaluation of Their Effects on Feed Efficiency

Profiling of Chromatin Accessibility in Pigs across Multiple Tissues and Developmental Stages

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