Differences in gut microbiota associated with age, sex, and stool consistency in healthy Japanese subjects

Takagi, Tomohisa; Naito, Yuji; Inoüe, Ryo; Kashiwagi, Saori; Uchiyama, Kazuhiko; Mizushima, Katsura; Tsuchiya, Saeko; Dohi, Osamu; Yoshida, Naohisa; Kamada, Kazuhiro; Ishikawa, Takeshi; Handa, Osamu; Konishi, Hideyuki; Okuda, Kayo; Tsujimoto, Yoshimasa; Ohnogi, Hiromu; Itoh, Yoshito

doi:10.1007/s00535-018-1488-5

Cited by 207 publications

(181 citation statements)

References 40 publications

Supporting

Mentioning

159

Contrasting

Unclassified

Order By: Relevance

“…At the phylum level, previous reports have shown that people with a Westernized lifestyle had increased proportions of Firmicutes and decreased proportions of Bacteroidetes 12,14 , which is in accordance with the results of the present study. In this study, Bacteroidetes, not Firmicutes, predominated, which differed from previous observations in Japanese participants 27,28 . This difference between studies might be due to the difference in factors including BMI and physical activity of the participants, and methods such as bacterial deoxyribonucleic acid extraction from feces and next-generation sequencing.…”

Section: Discussioncontrasting

confidence: 99%

Alteration of gut microbiota by a Westernized lifestyle and its correlation with insulin resistance in non‐diabetic Japanese men

Yamashita

Okubo

Kobuke

et al. 2019

J of Diabetes Invest

View full text Add to dashboard Cite

Aims/IntroductionThe severity of insulin resistance is higher in Japanese‐American people with American lifestyles than in native Japanese people with Japanese lifestyles. Recently, the role of gut microbiota in the control of host metabolic homeostasis and organ physiology has been recognized. In addition, gut microbiota alterations have been suggested to contribute to pathogenesis of insulin resistance. The principle aim of the present study was to evaluate the impact of a Westernized lifestyle on the gut microbiota of Japanese‐Americans versus native Japanese, and its correlation with insulin resistance.Materials and MethodsA total of 14 native Japanese men living in Hiroshima, Japan, and 14 Japanese‐American men living in Los Angeles, USA, were included. A 75‐g oral glucose tolerance test was carried out for all participants to assess their glucose tolerance, and normal glucose tolerance was observed. We compared the insulin response with oral glucose load, the Matsuda Index, and the composition of the gut microbiota between the native Japanese and Japanese‐American men.ResultsJapanese‐American men showed higher area under the curve values for serum insulin concentrations during the oral glucose tolerance test and lower Matsuda Index than native Japanese men. Gut microbiota composition of the Japanese‐American men was different; in particular, they showed a relatively lower abundance of Odoribacter than native Japanese men. The ratio between relative abundance of Odoribacter and Matsuda Index was positively correlated between the two groups.ConclusionsOur findings suggest that Westernized lifestyles alter gut microbiota, and its alteration might induce insulin resistance in non‐diabetic Japanese men.

show abstract

Section: Discussioncontrasting

confidence: 99%

Alteration of gut microbiota by a Westernized lifestyle and its correlation with insulin resistance in non‐diabetic Japanese men

Yamashita

Okubo

Kobuke

et al. 2019

J of Diabetes Invest

View full text Add to dashboard Cite

show abstract

“…The genera Prevotella, Megagamonas, Fusobacterium, and Megasphaera were significantly increased in male subjects, while Bifidobacterium, Ruminococcus, and Akkermansia were significantly increased in female subjects. These results are consistent with the results of previous Japanese studies [7] [32], and may be considered as the characteristic of gender differences of composition of intestinal microbiota in the Japanese population.…”

Section: Gut Microbiota and Sex/gendersupporting

confidence: 92%

“…Similar to Vandeputte et al [29], we found a significant association between the bowel habits (stool shape and defecation frequency) and gut microbiota diversity. Furthermore, deviation of the gut microbiota composition in several genera, including Oscillospira, Ruminococcus, Anaerotruncus, Alistipes, and Akkermansia, in constipation subjects and Fusobacterium and Dorea in diarrhea subjects were confirmed, which are consistent with a previous report [32]. Although the role of these genera in stool consistency remains unclear, the results illustrate the effect of gut microbiota on stool consistency in Japanese healthy subjects.…”

Section: Gut Microbiota and Bowel Habits (Diarrhea/constipation)supporting

confidence: 90%

“…Considering the heterogeneity and bowel habits of the sample dataset, we excluded samples from individuals with diarrhea or constipation ( Supplementary Figure 1), which might cause bias in the reference range [29][30][31] [32]. Finally, 1319 samples were selected as the healthy reference dataset ( Supplementary Figure 1 (Table 4).…”

Section: Reference Ranges From Japanese Healthy Cohortmentioning

confidence: 99%

“…Recent reports have shown a clear difference in the composition of intestinal flora of infants, adults, and the elderly [7][8] [32]. The microbiota composition initially shifts after birth, followed by significant shifts during childhood and in later years [7] [8].…”

Section: Gut Microbiota and Age/generationmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of healthy reference ranges for clinically relevant gut microbial indicators: Mykinso cohort study in Japan

Watanabe¹,

Kameoka²,

Shinozaki³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Although several large cohort studies have focused on the human gut microbiome, large cohort study of the Japanese gut microbiome is scarce, especially that of healthy or nondiseased individuals. The purpose of this study was to establish a reference range for gut microbial indices by collecting Japanese real-world microbiome data from the web-based cohort.Methods: We collected stool samples and original survey on lifestyle from 5,843 Japanese people through web-based services. From the obtained 16S rRNA sequence data derived from the stool sample, the ratio and distribution of each taxa were analyzed. The relationship between different epidemiological attributes and gut microbial indicators were statistically tested.Results: In total, 100 taxa (genus level) of gut microbiota were shared by more than 10% of Japanese people, and 20 taxa (genus level) by 90% or more. Bacteroides-dominant type was the most popular entero-type. The qualitative and quantitative indicators of these common gut microbiota were confirmed to be strongly correlated with age, constipation / diarrhea, and a history of lifestyle-related diseases. Therefore, we set up the healthy sub-cohort that controlled for these attribute factors, and defined a reference range from the distribution of gut microbial index in that population.Conclusions: The gut microbiota of Japanese people had high beta diversity, and there was no single "typical" gut microbiota type. We believe that the reference range of the gut microbial index obtained in this study can be a new reference value for determining the balance and health of the gut microbiota of an individual. In the future, it is necessary to clarify the clinical validity of this reference value by comparing with the clinical disease cohort. Word count: 272 wordsKeywords: Gut microbiota, 16S rRNA, Japanese, reference range, microbial index Title of the project Draft No. Outline Confidential Page 4 of 29 Recently, several studies have revealed gender differences in gut microbiota [14][15][16][17]. Min et al [18] conducted an association study to identify bacterial compositions associated with men and women and showed similar microbiota characteristics, including overall abundance and diversity, between men and women. However, they also showed gender differences at the species level between microbial taxa related to fat distribution, suggesting the existence of a gender-specific microbiome signature corresponding to gender-specific fat distribution, which may also contribute to the observed sex-specific immunity differences [19]. Thus, several immune pathophysiology may be involved in gender differences in gut bacterial composition. Title of the project Draft No. Outline Confidential Page 5 of 29 5 Age is also an important factor affecting the gut microbiota [8][20][21][22]. Recent reports have described differences in gut microbiota between children and adults, and an adult-like composition of bacterial communities is established around 3-4 years old or more [7] [8][23][24][25]. In addition, intes...

show abstract

Effects of host identity on the gut microbiota: A comparative study on three microtinae species

Yao,

Zhao,

Tang

et al. 2024

Anim Models and Exp Med

View full text Add to dashboard Cite

BackgroundGut microbiota exert an immense effect on host health and host environmental adaptation. Furthermore, the composition and structure of gut microbiota are determined by the environment and host genetic factors. However, the relative contribution of the environment and host genetic factors toward shaping the structure of gut microbiota has been poorly understood.MethodsIn this study, we characterized the fecal microbial communities of the closely related voles Neodon fuscus, Lasiopodomys brandtii, and L. mandarinus after caged feeding in the laboratory for 6 months, through high‐throughput sequencing and bioinformatics analysis.ResultsThe results of pairwise comparisons of N. fuscus vs. L. brandtii and L. mandarinus vs. L. brandtii revealed significant differences in bacterial diversity and composition after domestication. While 991 same operational taxonomic units (OTUs) were shared in three voles, there were 362, 291, and 303 species‐specific OTUs in N. fuscus, L. brandtii, and L. mandarinus, respectively. The relative abundances of Proteobacteria and Prevotella, which are reported to be enriched in high‐altitude populations, were significantly higher in high‐altitude N. fuscus than in low‐altitude L. brandtii after domestication. Firmicutes, which produce various digestive enzymes for energy metabolism, and Spirochaetes, which can degrade cellulose, were found in higher abundance in subterranean L. mandarinus than that in L. brandtii which dwells on the earth surface.ConclusionOur findings showed that some components of gut microbiota still maintained dominance even when different host species are reared under the same environmental conditions, suggesting that these bacteria are substantially influenced by host factors..

show abstract

Differences in gut microbiota associated with age, sex, and stool consistency in healthy Japanese subjects

Abstract: Several changes in gut microbiota were associated with age and sex. Stool consistency and gut microbiota associations emphasized the importance of stool consistency assessments to understand intestinal function.

Cited by 207 publications

References 40 publications

Alteration of gut microbiota by a Westernized lifestyle and its correlation with insulin resistance in non‐diabetic Japanese men

Alteration of gut microbiota by a Westernized lifestyle and its correlation with insulin resistance in non‐diabetic Japanese men

Analysis of healthy reference ranges for clinically relevant gut microbial indicators: Mykinso cohort study in Japan

Effects of host identity on the gut microbiota: A comparative study on three microtinae species

Contact Info

Product

Resources

About