Differences in glycosaminoglycans synthesized by fibroblast-like cells from chick cornea, heart, and skin.

Conrad, Gary W.; Hamilton, Clayon B.; Haynes, Emily P

doi:10.1016/s0021-9258(17)39929-5

Cited by 110 publications

(6 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…( 6) Over half of the [3H]glucosamine incorporated into the glycosaminoglycan fraction is in a large (M >10000), unsulfated glycoconjugate which is resistant to sequential treatments capable of degrading all known types of glycosaminoglycans. Such a large proportion of glycosaminoglycan-like material, which is resistant to all of the sequential treatments degrading glycosaminoglycans, has not been observed in previous work with other cell types (Conrad et al, 1977;Hart, 1978;Lemkin & Farquhar, 1981). Related investigations suggest that this novel polysaccharide(s) is (are) made preferentially by the lymphocytes derived from the cortical region of the thymus and is (are) synthesized much less by the more mature medullary lymphocytes.2…”

Section: Discussionmentioning

confidence: 89%

“…Interestingly, most of the 3H radioactivity remains excluded from Sephadex G-50 after all of these treatments (Figure 3C,F). Unlike such material from other tissues (Hart, 1976(Hart, , 1978Conrad et al, 1977) this material is also totally resistant to degradation by keratanase,2 indicating that it is not undersulfated keratan sulfate and also that it is most probably unrelated to recently discovered unsulfated lactosaminoglycans such as erythroglycanin (Jarnefelt et al, 1978;Kukuda et al, 1979;Fukuda et al, 1981). This excluded material is also resistant to exhaustive neuraminidase digestion.2 Again, mitogenic activation of lymphocytes by concanavalin A stimulates the incorporation of radioactivity into both chondroitin sulfates and into this unidentified-resistant polysaccharide.…”

Section: Resultsmentioning

confidence: 98%

See 1 more Smart Citation

Biosynthesis of glycosaminoglycans by thymic lymphocytes. Effects of mitogenic activation

Hart¹

1982

Biochemistry

View full text Add to dashboard Cite

The immune response is regulated by cellular interactions involving thymus-derived lymphocytes. Even though evidence from several systems suggests that proteoglycans or their polysaccharide side chains, the glycosaminoglycans, are important mediators (modulators) of cellular interactions, little is known concerning the biosynthesis or possible functions of these macromolecules in lymphocytes. As an initial step in our systematic analyses of the complex arrays of protein saccharides of lymphocytes, the biosynthesis and secretion of glycosaminoglycans by both unstimulated and mitogenically activated lymphocytes have been investigated. Isolated thymic lymphocytes were labeled with D-[6-3H]-glucosamine and 35SO4(2-), and the amounts of radioactivity in each family of glycosaminoglycan or other types of saccharides were determined. The data indicate the following: (1) Lymphocytes synthesize and secrete substantial amounts of glycosaminoglycans. (2) Activated lymphocytes have greatly accelerated rates of secretion of glycosaminoglycans, which appear to be more highly sulfated than those of nonstimulated cells. (3) Sulfated glycosaminoglycans of lymphocytes consist largely of chondroitin 4-sulfates, with smaller amounts of heparan sulfates. (4) Lymphocyte stimulation results in a rapid and dramatic increase in the relative proportion of both cell-associated and cell-secreted chondroitin 6-sulfates. (5) Lymphocytes synthesize large proportions of an apparently unsulfated, glycosaminoglycan-like glycoconjugate which is resistant to sequential treatments degrading all known types of glycosaminoglycans. Taken together with previous work which indicates that exogenously added glycosaminoglycans are capable of altering lymphocyte functions, these data suggest that lymphocyte-derived glycosaminoglycans themselves may play an important role in modulating the cellular interactions which regulate the immune system.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 98%

Biosynthesis of glycosaminoglycans by thymic lymphocytes. Effects of mitogenic activation

Hart¹

1982

Biochemistry

View full text Add to dashboard Cite

show abstract

“…35S-GAGs were recovered from the column Vo with <5 % of alkali-treated material ineluded in the column. The HS and CS content of 35S-GAG samples were determined by sequential nitrous acid deaminative cleavage and chondroitinase ABC treatment by methods modified from Conrad et al (1977). Detergent-extracted 35S-macromoleculas resistant to the effects of nitrous acid and chondroitinase ABC digestion were considered 35S-glycoproteins (Brown et al, 1981) and this population never exceeded 10% of the detergent extract.…”

Section: Proteoglycan Purificationmentioning

confidence: 99%

Cell surface phosphatidylinositol-anchored heparan sulfate proteoglycan initiates mouse melanoma cell adhesion to a fibronectin-derived, heparin-binding synthetic peptide

Drake

Klein

Mickelson

et al. 1992

The Journal of Cell Biology

View full text Add to dashboard Cite

Abstract. Cell surface heparan sulfate proteoglycan (HSPG) from metastatic mouse melanoma cells initiates cell adhesion to the synthetic peptide FN-C/H II, a heparin-binding peptide from the 33-kD A chain-derived fragment of fibronectin. Mouse melanoma cell adhesion to FN-C/H II was sensitive to soluble heparin and pretreatment of mouse melanoma cells with heparitinase. In contrast, cell adhesion to the fibronectin synthetic peptide CS1 is mediated through an ot4B1 integrin and was resistant to heparin or heparitinase treatment.Mouse melanoma cell HSPG was metabolically labeled with [35S]sulfate and extracted with detergent.

show abstract

“…Indeed, although maximal levels of keratan sulfate biosynthesis are reached by day 9 (stage 35) of chick development, half-maximal levels already are present in the cornea by day 7 (stage 30) (22), i.e ., at the same stage at which the cornea stops making type III collagen (44) . In vivo, the fibroblasts make almost all of the keratan sulfate of the cornea (6,23), but they lose this ability within 24-48 h after their inoculation in vitro (6,7), a loss also seen in cultured corneal fibroblasts from other animals including humans (9,10,17,18,27,46,47). Corneal fibroblasts in vitro may, therefore, be free to begin synthesis of THE JOURNAL OF CELL BIOLOGY -VOLUME 84, 1980 type III collagen, for they would be losing or would have lost their ability to make keratan sulfate.…”

Section: Discussionmentioning

confidence: 99%

Synthesis of type III collagen by fibroblasts from the embryonic chick cornea

Conrad¹

1980

The Journal of Cell Biology

View full text Add to dashboard Cite

Synthesis of collagen types I, II, III, and IV in cells from the embryonic chick cornea was studied using specific antibodies and immunofluorescence . Synthesis of radioactively labeled collagen types I and III was followed by fluorographic detection of cyanogen bromide peptides on polyacrylamide slab gels and by carboxymethylcellulose chromatography followed by disc gel electrophoresis .Type III collagen had been detected previously by indirect immunofluorescence in the corneal epithelial cells at Hamburger-Hamilton stages 20-30 but not in the stroma at any age. Intact corneas from embryos older than stage 30 contain and synthesize type I collagen but no detectable type III collagen . However, whole stromata subjected to collagenase treatment and scraping (to remove epithelium and endothelium) and stromal fibroblasts from such corneas inoculated in vitro begin synthesis of type III collagen within a few hours while continuing to synthesize type I collagen . As demonstrated by double-antibody staining, most corneal fibroblasts contain collagen types I and III simultaneously . Collagen type III was identified biochemically in cell layers and media after chromatography on carboxymethylcellulose by detection of disulfide-linked al(III)3 by SDS gel electrophoresis .The conditions under which the corneal fibroblasts gain the ability to synthesize type III collagen are the same as those under which they lose the ability to synthesize the specific proteoglycan of the cornea: the presence of corneal-type keratan sulfate . KEY WORDS collagen -cornea fibroblasts -immunofluorescence " antibodiesThe factors that normally control the synthesis of type III collagen remain to be elucidated . Fibrous connective tissues generally contain both collagen type I and collagen type III, but the ratio between them varies with the tissue : the medial layer of the aorta contains, and aortic smooth muscle cells synthesize, -70% type III and^-30% type 1(5, 30) ; J . CELL BIOLOGY © The Rockefeller University Press " 0021-9525/80/03/0501/12 $1 .00 Volume 84 March 1980 501-512 dermis contains, and dermal fibroblasts synthesize, -10-20% type III and -80-90% type I (5, 13); tendon contains mostly type I (40) with some type III, which is located in the endotendineum (2) .Under constant conditions in vitro, the ratio between collagen types I and III synthesized remains constant for many cell generations (21) . However, changes in serum concentration or cell density can alter the ratio, with the highest proportions of type III collagen being synthesized in 501 on

show abstract

Differences in glycosaminoglycans synthesized by fibroblast-like cells from chick cornea, heart, and skin.

Cited by 110 publications

References 45 publications

Biosynthesis of glycosaminoglycans by thymic lymphocytes. Effects of mitogenic activation

Biosynthesis of glycosaminoglycans by thymic lymphocytes. Effects of mitogenic activation

Cell surface phosphatidylinositol-anchored heparan sulfate proteoglycan initiates mouse melanoma cell adhesion to a fibronectin-derived, heparin-binding synthetic peptide

Synthesis of type III collagen by fibroblasts from the embryonic chick cornea

Contact Info

Product

Resources

About