Differences in Gene Expression Profiles between Human Breast Tissue and Peripheral Blood Samples for Breast Cancer Detection

Abstract

The purpose of this study is to check the similarities of differential gene expression of 11 genes in breast cancer tissue and blood samples from the same individual for early detection of breast cancer. We had investigated differential gene expression by qRT-PCR in 20 breast cancer patients' tumoral tissues and corresponding blood sample. In our analysis BRCA2, HER-2, ER, PR, MET and BRAF mRNA levels were significantly over expressed in tumoral tissues. ER and PR mRNA levels were not detected in any of the peripheral blood samples, whereas KRAS and PTEN mRNA levels were not detected in any of the tumoral tissues. HER-2 (45%), EGFR (40%) and PI3KCA (30%). KRAS and PTEN mRNA levels were significantly over expressed in peripheral blood. In the correlation analysis expression of most of the genes were significantly altered in grade II and III in the tissues, where as in premenopausal women mRNA expression was significantly high in Grade II and III and ER/PR negative tumors. Our results suggests that BRCA2, ER, PR, PI3KCA, MET and BRAF differential gene expression at mRNA levels showed no diagnostic value as a marker of circulating tumour cells in breast cancer. qRT-PCR may be suitable alternative method for the determination of HER-2, EGFR, PI3KCA KRAS and PTEN mRNA status in the blood of breast cancer patients. Premenopausal women with high grade (Grade II and III) and ER/PR negative cases may be associated with proliferation/metastasis, high recurrence rate, and poor prognosis.

BRCA1 Gene Expression is Down Regulated in Both Familial and Sporadic Breast Cancer Cases in Baghdad- Iraq

“…All of the observations support the tumor suppressor role of BRCA1 gene in breast cancer development. The relatively increased BRCA1 expression in premenopausal patients in our study seems consistent with the findings of Kandula's and colleagues who showed an increase in BRCA1 expression in premenopausal women with grades II and III of breast cancer [38].…”

Cancer Biomarkers in Liquid Biopsy for Early Detection of Breast Cancer: A Systematic Review

“…In contrast, 26 articles reported sensitivity and specificity figures greater than 70% for combined biomarkers in the form of panels 27,29,30,61,76,77,81,82,87,90,93,94,97,98,101,104,106,111-114,118,123,124,132,147 leaving only 6 panels with figures lower than 70%. 61,76,93,114,124,132 Finally, 27 articles reported AUC and P values, 26,62,63,67,71,72,78,83,84,88,102,110,116,120,122,125,127,130,133,135,137,139,140,142,143,145,146 and of these, AUC was lower than 0.7 67,133,1...…”

Exploration of disease - specific biomarkers in cancer research by integrating biological knowledge and high throughput data

“…The purpose and contribution of C 3 is justified based on the consideration of tissue-specific differences in the expression of genes, which have been established in biological studies even for same-condition (homogeneous) population (variability of expression within and across populations) [Kandula et al, 2012, Schobesberger et al, 2008, Radich et al, 2004, Shin et al, 2011. Basically, the inclusion of C 3 aims to alleviate cross-tissue differences appearing in the base populations of control breast and control blood engaged in our study.…”