Differences in features of calcium transients between the nucleus and the cytosol in cultured heart muscle cells: analyzed by confocal microscopy

Minamikawa, Tetsuhiro; Takahashi, Akiyuki; Fujita, Setsuya

doi:10.1016/0143-4160(95)90031-4

Cited by 23 publications

(9 citation statements)

References 29 publications

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“…5-LO activation is calcium-dependent (30). In some cell types, changes in intranuclear calcium levels seem to be independent of changes in cytosolic calcium (31), whereas in other cell types, the two apparently are linked (32). Theoretically, an agonist that increased calcium exclusively in the cytosol would activate 5-LO (and initiate LT synthesis) in blood PMNs but not in elicited PMNs.…”

Section: Discussionmentioning

confidence: 99%

Rapid Import of Cytosolic 5-Lipoxygenase into the Nucleus of Neutrophils after in Vivo Recruitment and in Vitro Adherence

Brock¹,

McNish

Bailie

et al. 1997

Journal of Biological Chemistry

104

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5-Lipoxygenase catalyzes the synthesis of leukotrienes from arachidonic acid. The subcellular distribution of 5-lipoxygenase is known to be cell type-dependent and is cytosolic in blood neutrophils. In this study, we asked whether neutrophil recruitment into sites of inflammation can alter the subcellular compartmentation of 5-lipoxygenase. In peripheral blood neutrophils from rats, 5-lipoxygenase was exclusively cytosolic, as expected. However, in glycogen-elicited peritoneal neutrophils, abundant soluble 5-lipoxygenase was in the nucleus. Upon activation with calcium ionophore A23187, intranuclear 5-lipoxygenase translocated to the nuclear envelope. Elicited neutrophils required a greater concentration of A23187 for activation than did blood neutrophils (half-maximal response, 160 versus 52 nM, respectively) but generated greater amounts of leukotriene B 4 upon maximal stimulation (26.6 versus 7.68 ng/10 6 cells, respectively). Intranuclear 5-lipoxygenase was also evident in human blood neutrophils after adherence to a variety of surfaces, suggesting that adherence alone is sufficient to drive 5-lipoxygenase redistribution. These results demonstrate a physiologically relevant circumstance in which the subcellular distribution of 5-lipoxygenase can be rapidly altered in resting cells, independent of 5-lipoxygenase activation. Nuclear import of 5-lipoxygenase may be a universal accompaniment of neutrophil recruitment into sites of inflammation, and this may be associated with alterations in enzymatic function.

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Section: Discussionmentioning

confidence: 99%

Rapid Import of Cytosolic 5-Lipoxygenase into the Nucleus of Neutrophils after in Vivo Recruitment and in Vitro Adherence

Brock¹,

McNish

Bailie

et al. 1997

Journal of Biological Chemistry

104

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“…Thus the ratio of the emitted fluorescence intensities at 410 and 485 nm allows accurate estimation of [Ca 2ϩ ] i independent of optical path length or total dye concentration. Such ratio measurements also correct for uneven dye loading, dye leakage, photobleaching, and changes in cell volume, e.g., during contraction in muscle cells (43,252,416) (Fig. 2).…”

Section: Indomentioning

confidence: 99%

Measurement of Intracellular Calcium

Takahashi

Camacho

Lechleiter

et al. 1999

Physiological Reviews

671

511

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To a certain extent, all cellular, physiological, and pathological phenomena that occur in cells are accompanied by ionic changes. The development of techniques allowing the measurement of such ion activities has contributed substantially to our understanding of normal and abnormal cellular function. Digital video microscopy, confocal laser scanning microscopy, and more recently multiphoton microscopy have allowed the precise spatial analysis of intracellular ion activity at the subcellular level in addition to measurement of its concentration. It is well known that Ca2+ regulates numerous physiological cellular phenomena as a second messenger as well as triggering pathological events such as cell injury and death. A number of methods have been developed to measure intracellular Ca2+. In this review, we summarize the advantages and pitfalls of a variety of Ca2+ indicators used in both optical and nonoptical techniques employed for measuring intracellular Ca2+ concentration.

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“…2A and B), but its effect seems to be functionally negligible in terms of the time course of the physiological responses in oocytes. Ca2+ ions diffuse in and out of the nucleus probably through the nuclear pore, which is thought to be highly permeable for small molecules (Horowitz, 1972 (Carroll et al 1994;Gillot & Whitaker, 1994), as well as in many somatic cells (Giovannardi, Cesare & Peres, 1994;Lin et al 1994;O'Malley, 1994;Minamikawa et al 1995). Al-Mohanna et al (1994) reported that a relatively large [Ca2+], rise (> 300 nM)…”

Section: Resultsmentioning

confidence: 99%