Differences in fatty acid profiles and desaturation indices of abdominal subcutaneous adipose tissue between pregnant women with and without PCOS

Emami, Neda; Alizadeh, AliReza; Moini, Ashraf; Yaghmaei, Parichehreh; Shahhosseini, Maryam

doi:10.1080/21623945.2019.1710021

Cited by 7 publications

(7 citation statements)

References 35 publications

(57 reference statements)

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“…PUFA could modulate hormonal and lipid profiles of the body, lowered TG and cholesterol levels, patients with PCOS usually showed abnormal levels of PUFA metabolites. The study focused on differences in FA profiles of abdominal subcutaneous adipose tissue between pregnant women with and without PCOS found that total PUFA was lower in PCOS than non-PCOS women ( p < 0.004) [ 22 ]. The animal model study also showed that ω-3 PUFA had an effective role in improving lipid and hormonal profile, reducing blood glucose, body weight and histopathological damages in PCOS rats [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Untargeted metabolomic approach to study the serum metabolites in women with polycystic ovary syndrome

Tan²,

Zhuang³

et al. 2021

BMC Med Genomics

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Background Polycystic ovary syndrome (PCOS) is not only a kind of common endocrine syndrome but also a metabolic disorder, which harms the reproductive system and the whole body metabolism of the PCOS patients worldwide. In this study, we aimed to investigate the differences in serum metabolic profiles of the patients with PCOS compared to the healthy controls. Material and methods 31 PCOS patients and 31 matched healthy female controls were recruited in this study, the clinical characteristics data were recorded, the laboratory biochemical data were detected. Then, we utilized the metabolomics approach by UPLC-HRMS technology to study the serum metabolic changes between PCOS and controls. Results The metabolomics analysis showed that there were 68 downregulated and 78 upregulated metabolites in PCOS patients serum compared to those in the controls. These metabolites mainly belong to triacylglycerols, glycerophosphocholines, acylcarnitines, diacylglycerols, peptides, amino acids, glycerophosphoethanolamines and fatty acid. Pathway analysis showed that these metabolites were enriched in pathways including glycerophospholipid metabolism, fatty acid degradation, fatty acid biosynthesis, ether lipid metabolism, etc. Diagnosis value assessed by ROC analysis showed that the changed metabolites, including Leu–Ala/Ile–Ala, 3-(4-Hydroxyphenyl) propionic acid, Ile–Val/Leu–Val, Gly–Val/Val–Gly, aspartic acid, DG(34:2)_DG(16:0/18:2), DG(34:1)_DG(16:0/18:1), Phe–Trp, DG(36:1)_DG(18:0/18:1), Leu–Leu/Leu–Ile, had higher AUC values, indicated a significant role in PCOS. Conclusion The present study characterized the difference of serum metabolites and related pathway profiles in PCOS patients, this finding hopes to provide potential metabolic markers for the prognosis and diagnosis of this disease.

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Section: Discussionmentioning

confidence: 99%

Untargeted metabolomic approach to study the serum metabolites in women with polycystic ovary syndrome

Tan²,

Zhuang³

et al. 2021

BMC Med Genomics

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“…It is estimated 12 PCOS with 36 controls (for improving power of the study) with 1:3 ratio without any missing samples. Details of the sample collection were presented by Emami et al [ 22 ]. At the time of caesarean section, the surgeon excised 3–4 g of subcutaneous AT upon exposing the abdominal cavity.…”

Section: Methodsmentioning

confidence: 99%

Differences in expression of genes related to steroidgenesis in abdominal subcutaneous adipose tissue of pregnant women with and without PCOS; a case control study

Emami

Moini

Yaghmaei

et al. 2021

BMC Pregnancy Childbirth

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Background It was reported that steroid-related gene expressions in the adipose tissue (AT) of women differ between women affected with polycystic ovary syndrome (PCOS) and non-PCOS. Although association between PCOS in mother and offspring’s health is a crucial issue, there are few studies focusing on AT of pregnant women suffering from PCOS. Our objectives were to determine the differences between mRNA expression levels of key steroid-converting enzymes in abdominal subcutaneous AT of pregnant women afflicted with PCOS and non-PCOS. Methods Twelve pregnant women with PCOS (case) and thirty six non-PCOS pregnant women (control) (1:3 ratio; age- and BMI-matched) undergoing cesarean section were enrolled for the present study. Expressions of fifteen genes related to steriodogenesis in abdominal subcutaneous AT were investigated using quantitative real-time PCR. Results No significant differences were detected with respect to age, BMI (prior pregnancy and at delivery day), gestational period and parity among pregnant women with PCOS and non-PCOS. Most of the sex steroid-converting genes except 17β-Hydroxysteroid dehydrogenases2 (17BHSD2), were highly expressed on the day of delivery in subcutaneous AT. Women with PCOS showed significantly higher mRNA levels of steroidgenic acute regulator (STAR; P < 0.001), cytochrome P450 monooxygenase (CYP11A1; P < 0.05), 17α-hydroxylase (CYP17A1; P < 0.05), and 11β-Hydroxysteroid dehydrogenase (11BHSD1 and 11BHSD2; P < 0.05). The expression of steroid 21-hydroxylase (CYP21) in non-PCOS was fourfold higher than those of women with PCOS (P < 0.001). There were no significant differences between relative expression of aromatase cytochrome P450 (CYP19A1), 3β-hydroxysteroid dehydrogenase (3BHSD1 and 3BHSD2), and 17BHSD family (1, 3, 5, 7, and 12) between the two groups. Conclusion The expression levels of genes related to sex steroids metabolism were similar to age-matched and BMI- matched pregnant non-PCOS and pregnant women with PCOS at delivery day. However, the alterations in gene expressions involved in glucocorticoids and mineralocorticoids metabolism were shown. It is necessary to point out that further studies regarding functional activity are required. More attention should be given to AT of pregnant women with PCOS that was previously ignored.

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“…When performing the laparoscopy, all patients were fasting, and the surgeon removed 3-4 g of subcutaneous fat. Biopsies were immediately rinsed in cooled NaCl 0.9% solution, segmented, and snap-frozen as previously reported (9,18). Until analysis, samples were kept frozen in liquid nitrogen (196°C).…”

Section: Sample Preparationmentioning

confidence: 99%

“…Adipose tissue (AT) has significant endocrine and metabolic functions as is known as an organ for storing fat (8). Extensive research has been done on the functions of subcutaneous AT in PCOS women (9,10). Beyond proteins, adipocytokines-which are released by AT-also involve lipids, extracellular vesicles, metabolites, and noncoding RNAs in the process whereby AT communicates with other body organs (11).…”

Section: Introductionmentioning

confidence: 99%

Differences in metabonomic profiles of abdominal subcutaneous adipose tissue in women with polycystic ovary syndrome

Gao²,

Yao³

et al. 2023

Front. Endocrinol.

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IntroductionPolycystic ovary syndrome (PCOS) is a complex endocrine disorder that often coexists with a metabolic disorder. Studies have demonstrated that the malfunction of adipose tissue, particularly abdominal adipose tissue, could exacerbate reproductive and metabolic problems in PCOS patients. Adipose tissue-secreted signaling mediators (e.g., lipids and metabolites) would then interact with other body organs, including the ovary, to maintain the systemic equilibrium.MethodsIn this study, we examined adipose samples from PCOS patients and unaffected individuals using a liquid chromatography–mass spectrometry-based metabonomics approach (LC–MS/MS). PCOS biomarkers were selected using multivariate statistical analysis.ResultsOur pathway analysis revealed that these differential metabolites could be engaged in inflammatory diseases and mitochondrial beta-oxidation. We further developed an in vitro PCOS cell model to examine the effects of hyperandrogenism on granulosa cells and related metabolic disorders. We noted that isoleucine recovered the promotive effect on cell apoptosis, inhibitory effect on cell proliferation, sex hormone secretion, and mitochondrial function induced by dehydroepiandrosterone. Our gas chromatography–mass spectrometry targeted analysis (GC–MS/MS) revealed that isoleucine was significantly decreased in PCOS patients.DiscussionBased on these results, we speculate that metabolome alterations are vital in ameliorating PCOS symptoms. This may be a novel therapeutic target for PCOS treatment. Our study provides preliminary evidence that these findings will enhance our ability to accurately diagnose and intervene in PCOS.

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Differences in fatty acid profiles and desaturation indices of abdominal subcutaneous adipose tissue between pregnant women with and without PCOS

Cited by 7 publications

References 35 publications

Untargeted metabolomic approach to study the serum metabolites in women with polycystic ovary syndrome

Untargeted metabolomic approach to study the serum metabolites in women with polycystic ovary syndrome

Differences in expression of genes related to steroidgenesis in abdominal subcutaneous adipose tissue of pregnant women with and without PCOS; a case control study

Differences in metabonomic profiles of abdominal subcutaneous adipose tissue in women with polycystic ovary syndrome

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