Differences in DNA composition along mammalian metaphase chromosomes

Chapelle, Albert de la; Schröder, Jim; Selander, Ritva Kajsa; Stenstrand, K

doi:10.1007/bf00399406

Cited by 46 publications

(4 citation statements)

References 36 publications

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“…A modification of the procedure, termed T -banding, stains only telomeres (122). Subsequent studies demonstrated that staining the treated chromosomes with acridine orange resulted in excellent R banding (26, [123][124][125][126][127][128][129]. When photographed in color the R-bands were green, the remaining portions of the chromosome were orange or red.…”

Section: R-banding Detection Of Gc-rich Dnamentioning

confidence: 99%

Mechanisms of Chromosome Banding and Implications for Chromosome Structure

Comings¹

1978

Annu. Rev. Genet.

266

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Section: R-banding Detection Of Gc-rich Dnamentioning

confidence: 99%

Mechanisms of Chromosome Banding and Implications for Chromosome Structure

Comings¹

1978

Annu. Rev. Genet.

266

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“…In a study of mouse and human chromosomes by the C-banding technique, CHEN and RUDDLE (1971) showed that metacentric mouse chromosomes contained about twice the normal amount of centric hvterochromatin. This has been confirmed by the use of a new fluorescent compound (HILWIG ahd GROPP 1972) and by acridine orange staining after denaturation-reassociation (DE LA CHAPELLE et al 1973). Furthermore, on mouse chromosomes there are several instances of heterochromatin displaced from the centromere but staining deeply by the C-banding technique and annealing with mouse satellite DNA (CHEN and RUDDLE 1971), and also staining brightly with the compound detected by HILWG and GROPP (1972).…”

Section: Discussionmentioning

confidence: 80%

Dicentric human X chromosomes

Chapelle

Stenstrand

2009

Hereditas

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Cytological findings in 5 individuals with structurally abnormal X chromosomes are presented. Of 4 patients with an i(Xq) chromosome, 2 had an apparently dicentric, and 2 an apparently monocentric isochromosome. The former type of chromosome had twice as much centric heterochromatin (2 distinct C‐bands) as the latter. In the apparently dicentric isochromosomes, it could not be determined whether one or both centromeres were functional. These findings confirm our previously presented hypothesis that transverse breakage of the X chromosome in the centromere region leading to isochromosome formation may occur at more than one site. A 60‐year‐old female, 154 cm in height, with primary amenorrhoea and no other signs of Turner's syndrome, had 46 chromosomes, including a normal X and a giant chromosome composed of two X chromosomes joined end to end by their short arms. By conventional staining this chromosome had 1 centromere, but C‐banding revealed centric heterochromatin both in the centromere and at the site where the centromere of the second component chromosome should have occurred. It is inferred that centric heterochromatin can occur elsewhere than at the centromere and is not necessarily associated with kinetochore function.

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“…This assumption is supported by observations of the quenching of HO fluorescence of BrdUrd substituted DNA of whole cells (2,21) and of chromosomes isolated from cells treated with BrdUrd (7). It should be noted, however, that the effect of BrdUrd substitution on energy transfer from HO to CA3 (19) and the nonrandom distribution of nucleotides in the various chromosomes (8,18,(22)(23)(24) have not been considered in these calculations. Using these simplifying assumptions we estimated the fraction of HO binding DNA synthesized during various periods of BrdUrd labeling (due to the HO binding specificity for runs of three adenine (A)/thymine (T) pairs, this relates to the amount of AT replicated).…”

Section: Resultsmentioning

confidence: 84%

Replication kinetics of Chinese hamster chromosomes as revealed by bivariate flow karyotyping

Cremer

Gray

1983

Cytometry

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In this paper we show that the replication kinetics of individual chromosomes can be estimated by bivariate flow cytometric analysis of chromosomes isolated from 5-bromo-Z'-deoxyuridine (BrdUrd) treated cells. To study the timing of replication of chromosomes, Chinese hamster M3-1 cells were grown for different times in BrdUrd containing medium. Colcemid was added during the last 90 minutes of each BrdUrd labeling period. Chromosomes were isolated from the

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Differences in DNA composition along mammalian metaphase chromosomes

Cited by 46 publications

References 36 publications

Mechanisms of Chromosome Banding and Implications for Chromosome Structure

Mechanisms of Chromosome Banding and Implications for Chromosome Structure

Dicentric human X chromosomes

Replication kinetics of Chinese hamster chromosomes as revealed by bivariate flow karyotyping

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