Differences in Detected Fluorescence Among Several Bacterial Species Measured with a Direct-Reading Particle Sizer and Fluorescence Detector

Brosseau, Lisa M.; Vesley, Donald; Rice, Nancy R.; Goodell, Karen; Nellis, Melissa A.; Hairston, Peter

doi:10.1080/027868200303461

Cited by 83 publications

(79 citation statements)

References 11 publications

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“…The Ultraviolet Aerodynamic Particle Sizer 5 (UV-APS) was the first commercially available, fluorescence-based instrument for real-time analysis of biological aerosols (Hairston et al, 1997;Brosseau et al, 2000). The UV-APS measures the aerodynamic diameter and side scatter parameter (analogous to the optical diameter) of incoming particles by measuring their time-of-flight between two lasers (633 nm) and then detects fluorescence in the wavelength range of 420Á575 nm after excitation by a pulsed ultraviolet laser (Nd:YAG, 355 nm).…”

Section: Modern Analysis Methodsmentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,170

1,114

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A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.

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Section: Modern Analysis Methodsmentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,170

1,114

View full text Add to dashboard Cite

show abstract

“…As a result, a number of real-time and commercial instruments including the Ultraviolet Aerodynamic Particle Sizer (UV-APS; TSI Inc., Shoreview, MN, USA) and the Waveband Integrated Bioaerosol Sensor (WIBS; Droplet Measurement Technologies, Longmont, CO, USA) are being commonly used in bioaerosol research communities (e.g., Agranovski et al, 2003;Bhangar et al, 2014;Brosseau et al, 2000;Foot et al, 2008;Huffman et al, 2010;Perring et al, 2015;Stanley et al, 2011;Toprak and Schnaiter, 2013). The main principle common to these techniques is the detection of intrinsic fluorescence from fluorophores such as amino acids, coenzymes, vitamins, and pigments that ubiquitously occur in aerosols of biological origin (e.g., Hill et al, 2009;Pan et al, 2010;Pöhlker et al, 2012Pöhlker et al, , 2013.…”

Section: Introductionmentioning

confidence: 99%

Characterization of steady-state fluorescence properties of polystyrene latex spheres using off- and online spectroscopic methods

et al. 2018

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Abstract. Fluorescent dyed polystyrene latex spheres (PSLs) are commonly used for characterization and calibration of instruments detecting fluorescence signals from particles suspended in the air and other fluids. Instruments like the Ultraviolet Aerodynamic Particle Sizer (UV-APS) and the Waveband Integrated Bioaerosol Sensor (WIBS) are widely used for bioaerosol research, but these instruments present significant technical and physical challenges requiring careful characterization with standard particles. Many other research communities use flow cytometry and other instruments that interrogate fluorescence from individual particles, and these also frequently rely on fluorescent PSLs as standards. Nevertheless, information about physical properties of commercially available PSLs provided by each manufacturer is generally proprietary and rarely available, making their use in fluorescence validation and calibration very difficult.This technical note presents an overview of steady-state fluorescence properties of fluorescent and non-fluorescent PSLs, as well as of polystyrene-divinylbenzene (PS-DVB) particles, by using on-and offline spectroscopic techniques. We show that the "fluorescence landscape" of PSLs is more complex than the information typically provided by manufacturers may imply, especially revealing multimodal emission patterns. Furthermore, non-fluorescent PSLs also exhibit defined patterns of fluorescent emission originating from a mixture of polystyrene and detergents, which becomes a crucial point for fluorescence threshold calibrations and qualitative comparison between instruments. By comparing PSLs of different sizes, but doped with the same dye, changes in emission spectra from bulk solutions are not immediately obvious. On a single-particle scale, however, fluorescence intensity values increase with increasing particle size. No significant effect in the fluorescence signatures was detectable by comparing PSLs in dry vs. wet states, indicating that solvent water may only play a minor role as a fluorescence quencher.Because information provided by manufacturers of commercially available PSLs is generally very limited, we provide the steady-state excitation-emission matrices (EEMs) of PSLs as open-access data within the Supplement. Detergent and solvent effects are also discussed in order to provide information not available elsewhere to researchers in the bioaerosol and other research communities. These data are not meant to serve as a fundamental library of PSL properties because of the variability of fluorescent properties between batches and as a function of particle aging and agglomeration. The data presented, however, provide a summary of spectral features which are consistent across these widely used fluorescent standards. Using these concepts, further checks will likely be required by individual researchers using specific lots of standards.

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“…LIF is particularly useful for detecting biological molecules within aerosol particles Hairston et al 1997;Eversole et al 1999;Reyes et al 1999;Brosseau et al 2000;Kaye et al 2000;Ho 2002). These efforts concentrate on the measurement of the undispersed fluorescence of particles and consequently only have limited potential for providing information on particle composition.…”

Section: Introductionmentioning

confidence: 99%

Single-Particle Fluorescence Spectrometer for Ambient Aerosols

Pan

Hartings

Pinnick

et al. 2003

Aerosol Science and Technology

109

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A fluorescence particle spectrometer (FPS) for real-time measurement of the fluorescence spectra of aerosol particles in the size range 1-10 µm diameter is reported. The prototype FPS has a sufficiently high sample rate (from 5 to 28 l/min for 3.5 µm to 11 µm diameter particles) to measure aerosol within buildings at practical rates (from 1 up to 600 particle fluorescence spectra per minute). Previously reported bioaerosol prototype detectors for measurement of single particle spectra (Pan et al., Opt. Lett., 24, 116-118 (1999); Hill et al., Field Anal. Chem. Tech., 3, 221-239 (1999)) were unable to sample the ambient environment; air containing particles had to be forced under pressure into a sample cell. In addition, sample rates were so small (less than 0.01 l/min) as to be impractical for most applications. The present design overcomes these deficiencies by the use of an airtight cell that highly concentrates micrometer-sized particles. A virtual impactor first concentrates aerosol particles, which are then drawn under negative pressure through an aerodynamic focusing nozzle in the inlet of the instrument, through the sample region, providing further concentration. The rate of particle spectra measured by the FPS increases significantly when the particle inlet is within a few meters of some common sources of indoor biological particles, e.g., a person coughing, sneezing, or rubbing his skin, or the presence of a dog. The spectra obtained have a variety of spectral shapes. The FPS may be useful in a variety of areas, e.g., in studying and monitoring airborne particles that cause diseases or allergies.

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Differences in Detected Fluorescence Among Several Bacterial Species Measured with a Direct-Reading Particle Sizer and Fluorescence Detector

Cited by 83 publications

References 11 publications

Primary biological aerosol particles in the atmosphere: a review

Primary biological aerosol particles in the atmosphere: a review

Characterization of steady-state fluorescence properties of polystyrene latex spheres using off- and online spectroscopic methods

Single-Particle Fluorescence Spectrometer for Ambient Aerosols

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