Differences in clinical manifestations of genital chlamydial infections related to serovars.

Laar, M J van de; Duynhoven, Y T van; Fennema, J. S. A.; Ossewaarde, Jacobus M.; Brule, A J van den; Doornum, G J van; Coutinho, Roel A.; Hoek, J. van den

doi:10.1136/sti.72.4.261

Cited by 31 publications

(37 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in agreement with other studies performed in different parts of the world (8,15,17,18). With the RLB developed we found that 91.3% of the women showed single C. trachomatis infections and 8.7% showed multiple infections.…”

Section: Discussionsupporting

confidence: 82%

“…The size of the fragment generated in this PCR is rather large (1.1 kb), which makes this omp1 RFLP assay sensitive for DNA degradation, thus explaining the low level of detection with this technique. Therefore, due to the small size of amplified product, the VD2 PCR-RLB assay is suitable for the analysis of less-preserved material such as archival smears and paraffin-embedded tissues, as previously also shown for the typing of human papillomaviruses with PCR-RLB (15).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

et al. 2004

View full text Add to dashboard Cite

In this study we developed and evaluated a new PCR-based typing assay, directed to the VD2 region of the omp1 gene, for the detection and typing of urogenital Chlamydia trachomatis infections. A nested VD2 PCRreverse line blot (RLB) assay was developed for the typing of nine different urogenital serovars of C. trachomatis. The assay developed was tested with reference strains of C. trachomatis serovars and cervical scrapes of 86 Colombian women previously found to be positive for C. trachomatis by using plasmid PCR. Two sets of primers directed to the VD2 region of the omp1 gene of C. trachomatis were designed, and fragments of 220 and 166 bp were generated in the primary and nested PCRs, respectively. In addition, an RLB assay was developed to identify nine different urogenital serovars of C. trachomatis (Ba, D, E, F, G, H, I, J, and K) and group controls, including group B (Ba, D, and E), group C (I, J, K, and H), and an intermediate group (F and G). Using this assay, we were able to type 81 of the 86 samples (94.2%). Of these samples, 91.3% were single C. trachomatis infections, and 8.7% were multiple infections. The most common serovars identified were serovars D (22.2%), F (18.5%), G (13.6%), and E (12.3%). Of the women with multiple C. trachomatis infections, >50% contained both serovars D and E. The nested VD2 PCR-RLB developed is a simple, fast, and specific method for the identification of individual urogenital C. trachomatis serovars previously detected by using plasmid PCR. Moreover, it is an appropriate method for studying multiple C. trachomatis infections and for use in large epidemiological studies.Chlamydia trachomatis is one of the most common pathogens of sexually transmitted diseases worldwide.Initially, 15 prototypic serovars labeled A to K and L1, L2, and L3 were identified based on immunoepitope analysis of the major outer membrane protein (MOMP) with monoclonal and polyclonal antibodies (7, 10). On the basis of the pathogenic potential, the serovars A, B, Ba, and C are commonly associated with trachoma, D to K are commonly associated with urogenital infections, and L1, L2, and L3 are commonly associated with lymphogranuloma venereum. In addition, based on amino acid similarity, the serovars have been grouped in three different serogroups: group B (B, Ba, D, Da, E, L1, L2, and L2a), group C (A, C, H, I, Ia, J, Ja, K and L3), and an intermediate group (F and G) (20).The typing of C. trachomatis infection is important for epidemiological and vaccination studies. In addition, the knowledge of pathogenesis and transmission rates of C. trachomatis serovars are important topics in clinical and basic research (1,3,14).To study the epidemiology of C. trachomatis infections, different laboratory techniques for C. trachomatis serovar identification have been developed in the last few years. These techniques include standard MOMP serotyping, restriction fragment length polymorphism (RFLP) analysis of the PCRamplified omp1 gene, and nucleotide sequencing of the omp1 gene (3,4,12,13,19). Compared to imm...

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

et al. 2004

View full text Add to dashboard Cite

show abstract

“…This compares favorably with failure rates of 1 to 24% in similar studies (17,18,31). The serotype distribution in our study was similar to that found in other investigations based on the use of monoclonal antibodies (23,35), restriction fragment length polymorphism (25,36), or gene sequencing (17,18). The study of Jurstrand et al (18) took place in a neighboring county less than a year prior to our study, so it is noteworthy that only 2.5% of their cases, compared with 11% of ours, were of serotype G. This difference is probably due to fluctuations of serotypes within the population over time, since genotype G constituted 4% of the strains in Värmland in January and 20% in December (P Ͻ 0.001).…”

Section: Discussionsupporting

confidence: 80%

“…This supports our finding of a lack of association between serotype and clinical symptoms. Previous conflicting data have suggested weak support for an association between symptoms and some serotypes or complexes (3,9,35). The two largest studies, however, found no (24) or only weak (12) evidence for such an association.…”

Section: Discussionmentioning

confidence: 86%

Characterization of ompA Genotypes by Sequence Analysis of DNA from All Detected Cases of Chlamydia trachomatis Infections during 1 Year of Contact Tracing in a Swedish County

Lysén¹,

et al. 2004

View full text Add to dashboard Cite

In this study we aimed to characterize the ompA gene by sequencing DNA from all detected cases of Chlamydia trachomatis infection in a Swedish county during 2001, in order to improve the efficiency of contact tracing. Approximately 990 bp of the ompA gene was amplified, and sequence analysis was achieved in 678 (94%) of 725 C. trachomatis-positive cases in this unselected population. The most prevalent genotype was serotype E (39%), followed by F (21%), G (11%), D (9%), K (9%), J (7%), H (2%), B (1%), and Ia (1%). Serotype E was found in five genotype variants, with the reference sequence comprising 96% of all E cases. Serotype D was the most variable, and of seven sequence variants, three were identified as recombinants with serotype E. Altogether 29 genetic variants were detected, and mutations and recombination events are discussed. Clinical manifestations were not associated with genotypes. Sequence variation was linked to sexual networks identified by contact tracing and improved epidemiological knowledge but was of limited benefit.

show abstract

“…Using identical probes, Molano et al reported that 9% of specimens from women in Colombia contained more than one C. trachomatis serovar (12). Many other studies from different parts of the world (12)(13)(14)(15)(20)(21)(22) have shown small proportions of specimens with mixed serovars (2 to 15%) (1,5,6,13,14). Using a reverse dot blot assay, Stothard (17) was able to identify both C. trachomatis serovars in eight artificially mixed specimens and seven of eight clinically mixed specimens (each containing two serovars), as identified by immunofluorescence.…”

Section: Discussionmentioning

confidence: 99%

Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis

Xiong

Kong

Zhou³

et al. 2006

J Clin Microbiol

View full text Add to dashboard Cite

The aim of this study was to develop and evaluate multiplex and nested PCR-reverse line blot (RLB) hybridization assays for detection and serovar identification of Chlamydia trachomatis. Two sets of primers targeting the VD2 region of the omp1 gene and one set targeting the cryptic plasmid were designed for use in multiplex (both targets) and nested PCR (omp1 only). For the RLB assay, labeled omp1 amplicons were hybridized to a membrane containing probes specific for 15 C. trachomatis serovars. The assays were used to test 429 clinical specimens, which had been previously tested for C. trachomatis using the COBAS AMPLICOR system. Specimens were tested without knowledge of the COBAS AMPLICOR result. Of 205 specimens that were positive by COBAS AMPLICOR, 201 (98%) were positive by multiplex PCR-RLB and 188 (92%) were also positive by omp1 nested PCR-RLB. In addition, three of 224 COBAS AMPLICOR-negative specimens were positive by omp1 nested PCR-RLB. One hundred sixty-six of 191 (87%) specimens in which C. trachomatis serovars were identified contained only one serovar and 25 (13%) contained two or three serovars. Serovars D, E, and F were found in 31 (16%), 83 (43%), and 51 (27%) specimens, respectively. Serovar E (41%) was the most commonly identified single serovar. Serovars J and K were found alone uncommonly (<2% each), but 18 of 25 (72%) specimens with multiple C. trachomatis serovars contained one or both (10 specimens) of these serovars. The nested (ompI) PCR-RLB is a specific and sensitive method for simultaneous detection and serovar identification of C. trachomatis, which can reliably identify mixed C. trachomatis serovars. It is suitable for use in epidemiological studies.

show abstract

Differences in clinical manifestations of genital chlamydial infections related to serovars.

Cited by 31 publications

References 24 publications

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

Combination of PCR Targeting the VD2 of omp1 and Reverse Line Blot Analysis for Typing of Urogenital Chlamydia trachomatis Serovars in Cervical Scrape Specimens

Characterization of ompA Genotypes by Sequence Analysis of DNA from All Detected Cases of Chlamydia trachomatis Infections during 1 Year of Contact Tracing in a Swedish County

Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis

Contact Info

Product

Resources

About