Differences in Caco-2 cell attachment, migration on collagen and fibronectin coated polyelectrolyte surfaces

Zanina, Nahla; Mora, Laurence; Othmane, Ali; Bénard, Magalie; Duncan, A.C.; Jouenne, Thierry; Vaudry, David; Souiri, Mina

doi:10.1007/s12257-012-0207-y

Cited by 7 publications

(4 citation statements)

References 48 publications

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“…B). Although other TLVM studies document live Caco2 cell phenomena , they often indicate merely that the cells were incubated on a heated stage or in a chamber at 37 degrees Celsius and do not demonstrate that the cells themselves experience a physiological environment and behave as expected under physiological conditions. Our device measures conditions in the immediate environment surrounding the cells and recapitulates the pH, humidity, and cell growth rate maintained by a standard incubator.…”

Section: Discussionmentioning

confidence: 99%

Novel aspects of live intestinal epithelial cell function revealed using a custom time‐lapse video microscopy apparatus

Papetti

Kozlowski

2018

Cytometry Pt A

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Many aspects of cell physiology, including migration, membrane function, and cell division, are best understood by observing live cell dynamics over time using video microscopy. To probe these phenomena in colon epithelial cells using simple components with a limited budget, we have constructed an inexpensive (<$410) self-contained apparatus, consisting of a closed-loop, feedback-controlled system regulated by a PID (proportional-integrative-derivative) controller contained within a 0.077 m insulated acrylic box. Temperature, humidity, pH, and proliferative capacity of colon epithelial cells in this system mimic those in a standard tissue culture incubator for over four days. Our system offers significant advantages over existing cost-prohibitive commercially available and custom-made devices because of its very low cost, use of PID temperature control, lack of reliance on constant infusion of external humidified, heated air or carbon dioxide, ability to directly measure cell culture medium temperature, and combination of exquisite cellular detail with minimal focus drift under physiological conditions for extended periods of time. Using this apparatus, coupled with an inverted microscope equipped with phase contrast optics and a programmable digital camera, we have observed many events in colon epithelial cells not visible by static imaging, including kinetics of normal and abnormal mitoses, dynamic membrane structures, intracellular vesicle movements, and cell migration. © 2018 International Society for Advancement of Cytometry.

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Section: Discussionmentioning

confidence: 99%

Novel aspects of live intestinal epithelial cell function revealed using a custom time‐lapse video microscopy apparatus

Papetti

Kozlowski

2018

Cytometry Pt A

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“…For example, polysaccharides extracted from mushrooms, including Agaricus blazei, Phellinus linteus, Hericium erinaceus, and I. obliquus exhibit important pharmacological properties. Tumor metastasis is a multi-step process, with complex regulation, which includes angiogenesis, cell attachment, adhesion, migration, invasion, and cell proliferation (25,26). Developing therapeutic agents that inhibit metastasis is considered key, however, effective anti-metastatic agents require further research.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharide isolated from the liquid culture broth of Inonotus obliquus suppresses invasion of B16-F10 melanoma cells via AKT/NF-κB signaling pathway

Lee

et al. 2016

Molecular Medicine Reports

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A number of polysaccharides exhibit pharmacological activities. Polysaccharides derived from Inonotus obliquus (PLIO) appear to have various potential pharmacological properties, including anti‑tumor activity. However, the molecular mechanisms underlying these properties remain to be elucidated. The present study investigated the anti‑metastatic potential of PLIO and the underlying signaling pathways in B16‑F10 murine melanoma cells using the MTT colorimetric assay, in vitro migration and invasion assays, and flow cytometric and western blot analyses. PLIO inhibited the invasion of B16‑F10 cells and suppressed the expression of matrix metalloproteinases. PLIO treatment inhibited nuclear factor‑κB (NF‑κB) nuclear translocation in B16‑F10 cells. In addition, PLIO treatment inhibited the phosphorylation of c-Jun N‑terminal kinases and AKT. These results suggest that PLIO may suppress the invasion of highly metastatic melanoma cells via inhibition of the AKT/NF-κB signaling pathways.

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“…Initial physico-chemical surface modification may significantly improve substrate-biological media interactions, while maintaining desired material bulk mechanical properties. Much work on the subject of surface modification for subsequent immobilization of adhesive and/or bioactive proteins or biomolecules has been done [4][5][6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…Although, this former approach has been abundantly described in the literature for surface immobilization of cell adhesive RGD amino acid sequences, peptides or FN fragments, it has been more scarcely for surface immobilization of the whole pFN molecule. This may be due to the purification cost of human plasma FN (pFN) [32,8] and other associated problematic issues such as virus or prion contamination risks.…”

Section: Introductionmentioning

confidence: 99%

Protein covalent immobilization via its scarce thiol versus abundant amine groups: Effect on orientation, cell binding domain exposure and conformational lability

Hindié²,

Marmey

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Differences in Caco-2 cell attachment, migration on collagen and fibronectin coated polyelectrolyte surfaces

Cited by 7 publications

References 48 publications

Novel aspects of live intestinal epithelial cell function revealed using a custom time‐lapse video microscopy apparatus

Novel aspects of live intestinal epithelial cell function revealed using a custom time‐lapse video microscopy apparatus

Polysaccharide isolated from the liquid culture broth of Inonotus obliquus suppresses invasion of B16-F10 melanoma cells via AKT/NF-κB signaling pathway

Protein covalent immobilization via its scarce thiol versus abundant amine groups: Effect on orientation, cell binding domain exposure and conformational lability

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