To assess the intrarater reliability and construct validity of the timed stair test (TST) for patients after unilateral total knee arthroplasty (TKA). [Participants and Methods] The validity of the TST was assessed using 37 patients who had undergone unilateral TKA, and the reliability of the TST was assessed using 14. The time taken to perform the TST using training stairs (at riser heights of 15 and 20 cm each) was compared in terms of patients' physical function and background factors. Multiple regression analysis was conducted with the TST score as the dependent variable. [Results] The intraclass correlation coefficient (1,1) of the TST score was 0.893 at a riser height of 15 cm, and 0.896 at a riser height of 20 cm. TST scores at both riser heights were significantly associated with non-operative knee extension strength and age, thereby supporting construct validity. [Conclusion] The reliability and construct validity of the TST were confirmed for unilateral TKA patients.