Differences in accumulation and the transport mechanism of l- and d-methionine in high- and low-grade human glioma cells

Kobayashi, Masato; Mizutani, Asuka; Nishi, Kodai; Nakajima, Syuichi; Shikano, Naoto; Nishii, Ryuichi; Fukuchi, Kazuki; Kawai, Keiichi

doi:10.1016/j.nucmedbio.2016.09.003

Cited by 14 publications

(12 citation statements)

References 6 publications

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“…In malignant gliomas, the glutamine metabolic remodeling is characterized by the abrogation of GS [ 74 ] and the increased expression of glutamine transporters such as ASCT2 [ 75 ] and SNAT3 [ 76 ]. Therefore, these neoplasms present an increased dependence on the import of glutamine upon the incapacity of producing it [ 77 ], acting as ‘glutamine traps’ by importing glutamine from the tumor microenvironment [ 78 ].…”

Section: Glutamine-glutamate Relevance In Cancermentioning

confidence: 99%

Take Advantage of Glutamine Anaplerosis, the Kernel of the Metabolic Rewiring in Malignant Gliomas

et al. 2020

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Glutamine is a non-essential amino acid that plays a key role in the metabolism of proliferating cells including neoplastic cells. In the central nervous system (CNS), glutamine metabolism is particularly relevant, because the glutamine-glutamate cycle is a way of controlling the production of glutamate-derived neurotransmitters by tightly regulating the bioavailability of the amino acids in a neuron-astrocyte metabolic symbiosis-dependent manner. Glutamine-related metabolic adjustments have been reported in several CNS malignancies including malignant gliomas that are considered ‘glutamine addicted’. In these tumors, glutamine becomes an essential amino acid preferentially used in energy and biomass production including glutathione (GSH) generation, which is crucial in oxidative stress control. Therefore, in this review, we will highlight the metabolic remodeling that gliomas undergo, focusing on glutamine metabolism. We will address some therapeutic regimens including novel research attempts to target glutamine metabolism and a brief update of diagnosis strategies that take advantage of this altered profile. A better understanding of malignant glioma cell metabolism will help in the identification of new molecular targets and the design of new therapies.

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Section: Glutamine-glutamate Relevance In Cancermentioning

confidence: 99%

Take Advantage of Glutamine Anaplerosis, the Kernel of the Metabolic Rewiring in Malignant Gliomas

et al. 2020

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“…This phenomenon could be explained by three reasons. For the first reason, even though D-Met could be metabolized by different types of cells [20][21][22][23], L-Met is the more favor one for cells uptake. In the current work, L-Met was not completely converted into SAM.…”

Section: Resultsmentioning

confidence: 99%

S-Adenosyl-l-methionine production by Saccharomyces cerevisiae SAM 0801 using dl-methionine mixture: From laboratory to pilot scale

Ren

Cai

et al. 2017

Process Biochemistry

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This study sought to develop a cost-effective biological catalysis process for S-adenosyl-L-methionine (SAM) production. During the process, a mixture of D-methionine (D-Met) and L-methionine (L-Met), namely DL-Met, was used as substrate to replace the conventional but expensive pure L-Met. The concentration of DL-Met in substrate was optimized. When 80 g/L of DL-Met was added in a 5 L-scale bioreactor, 13.74 g/L of SAM was produced with an L-Met conversion rate of 32.15%. The fermentation process was then scaled up to meet the requirements of realistic industrial SAM production. In a 300 L-scale fermentation process, 10.45 g/L of SAM was achieved, with an L-Met conversion rate of 32.61%. Moreover, the proportion of D-Met remaining in broth increased from 50% at the beginning to 76.89% at the end of fermentation. The fermentation process is generally appropriate for commercial SAM production.

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“…D-Methionine has no known activity in the brain but protect against toxicity of a variety of drugs as well as protecting against noise-induced hearing loss [ 71 , 155 , 156 ]. A recent study found that D-methionine is transported into the brain using system L and the Asc transporter [ 72 ]. In this study, D-methionine was able to concentrate in the brain to significantly higher levels than L-methionine suggesting that there is no endogenous system for metabolizing D-methionine in the brain [ 72 ].…”

Section: Inactive D-amino Acids With Interesting Derivatives or Otmentioning

confidence: 99%

“…A recent study found that D-methionine is transported into the brain using system L and the Asc transporter [ 72 ]. In this study, D-methionine was able to concentrate in the brain to significantly higher levels than L-methionine suggesting that there is no endogenous system for metabolizing D-methionine in the brain [ 72 ]. Recently, D-methionine was found to protect against ototoxicity in a variety of drug models by preventing the loss of connexin 26 and connexin 30 making it an interesting molecule for preventing drug induced cell death during pharmaceutical treatment [ 73 , 74 , 157 ].…”

Section: Inactive D-amino Acids With Interesting Derivatives or Otmentioning

confidence: 99%

Advances in D-Amino Acids in Neurological Research

Seckler

Lewis

2020

IJMS

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D-amino acids have been known to exist in the human brain for nearly 40 years, and they continue to be a field of active study to today. This review article aims to give a concise overview of the recent advances in D-amino acid research as they relate to the brain and neurological disorders. This work has largely been focused on modulation of the N-methyl-D-aspartate (NMDA) receptor and its relationship to Alzheimer’s disease and Schizophrenia, but there has been a wealth of novel research which has elucidated a novel role for several D-amino acids in altering brain chemistry in a neuroprotective manner. D-amino acids which have no currently known activity in the brain but which have active derivatives will also be reviewed.

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Differences in accumulation and the transport mechanism of l- and d-methionine in high- and low-grade human glioma cells

Cited by 14 publications

References 6 publications

Take Advantage of Glutamine Anaplerosis, the Kernel of the Metabolic Rewiring in Malignant Gliomas

Take Advantage of Glutamine Anaplerosis, the Kernel of the Metabolic Rewiring in Malignant Gliomas

S-Adenosyl-l-methionine production by Saccharomyces cerevisiae SAM 0801 using dl-methionine mixture: From laboratory to pilot scale

Advances in D-Amino Acids in Neurological Research

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