Differences between platelet and microparticle glycoprotein IIb/IIIa

Nomura, Shosaku; Suzuki, Masahiko; Kakemoto, Hirofumi; Yamaguchi, Kazuyuki; Fukuroi, Tsutomu; Yanabu, Mutsumasa; Soga, Tetsuji; Nagata, Hirokazu; Kokawa, Terutoshi; Yasunaga, Kōjirō

doi:10.1002/cyto.990130610

Cited by 53 publications

(25 citation statements)

References 40 publications

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“…PDMPs were detected with a modified version of a previously reported method [12,33,34]. Ten microlitres of platelets (3 10 8 /ml) added to 100 ml of HEPES-Tyrode's buffer containing 5 nmol/l EGTA.…”

Section: Methodsmentioning

confidence: 99%

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Detection of monocyte-derived microparticles in patients with Type II diabetes mellitus

et al. 2002

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Patients with diabetes mellitus develop hypercoagulability, platelet hyperaggregability and premature atherosclerosis [1±4]. Atherosclerosis is the major cause of death in patients with diabetes mellitus, leading to a high mortality rate in all forms of the disease [5]. Classical risk factors, including hyperlipidaemia, hypertension, and obesity, do not completely account for the increased incidence of atherosclerosis associated with diabetes [6]. Recent reports suggest that increased expression of endothelial adhesion Diabetologia (2002) Results. The concentration of MDMPs in diabetic patients was higher than in normal subjects. We found no differences in the binding of anti-GPIIb/IIIa and anti-GPIb monoclonal antibodies between groups. There were differences, however, in the concentrations of PDMPs, plt-CD62P, and plt-CD63 between Type II (non-insulin-dependent) diabetes mellitus patients and control subjects (PDMPs: 585 25 vs 263 9, p < 0.01; plt-CD62P: 28.1 % 1.4 % vs 9.4 % 0.6 %, p < 0.001; plt-CD63: 28.1 % 1.4 % vs 8.6 % 0.5 %, p < 0.001). Amounts of MDMPs correlated positively with these platelet activation markers, and the relation between PDMP and MDMP was the most significant. The concentration of MDMP in patients who had diabetes complicated with nephropathy, retinopathy, or neuropathy was higher than in those without diabetes-related complications. The increase in MDMP was particularly significant in patients with nephropathy. Concentrations of sE-selectin were higher in Type II diabetes patients than in control subjects, and correlated with MDMP, PDMP, plt-CD62P, and plt-CD63 levels in nephropathy patients. Conclusion/interpretation. In Type II diabetes patients, we detected increased activation of monocytes, which could have been stimulated by activated platelets and PDMPs. Because the activation of monocytes is associated with vascular endothelial damage, high concentrations of MDMPs could indicate vascular complications in diabetes patients, especially those who have diabetes-related nephropathy. [Diabetologia (2002) 45:550±555]

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Section: Methodsmentioning

confidence: 99%

“…As an index of platelet activation, CD62P expression was quantified by immunostaining with anti-CD62P monoclonal antibody (CLB-thromb/6, Immunotech, Marseille, France). Platelet expression of GPIIb/ IIIa and of GPIb was analysed with anti-GPIIb/IIIa (NNKY1±32) [33,36] and anti-GPIb (NNKY5±5) [37] monoclonal antibodies, respectively.…”

Section: Methodsmentioning

confidence: 99%

Detection of monocyte-derived microparticles in patients with Type II diabetes mellitus

et al. 2002

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“…Then the platelets were washed twice, resuspended in stock solution (9 m M Na 2 EDTA, 26.4 m M Na 2 HPO 4 2H 2 O, 140 m M NaCl, 0.1% NaN 3 , and 2% fetal bovine serum, pH 7.2), and stored at 4°C until analysis. PMP was detected with a modified version of a previously reported method [17, 18, 22, 23]. Ten microliters of platelet suspension (3 × 10 8 /ml) was added to 100 µl of Hepes-Tyrode’s buffer containing 5 nmol/l EGTA, and both intact and aggregated platelets were removed by centrifugation at 1,000 g for 15 min to yield a supernatant containing microparticles only.…”

Section: Methodsmentioning

confidence: 99%

Significance of Platelet-Derived Microparticles and Activated Platelets in Diabetic Nephropathy

Omoto¹,

Nomura²,

Shouzu³

et al. 1999

Nephron

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We measured levels of platelet-derived microparticles (PMP), which have coagulative activity and are produced by platelet activation or physical stimulation, and CD62P/CD63-positive platelets in patients with diabetes mellitus to determine their clinical significance and effects on complications of diabetes including diabetic nephropathy. We also compared these levels before and after administration of the antiplatelet drug cilostazol. Plasma PMP and CD62P/CD63-positive platelet levels were significantly higher in patients with diabetes mellitus than normal controls. CD62P-positive platelet levels were significantly higher in patients with nephropathy than in patients without complications. After administration of cilostazol, PMP and CD62P/CD63-positive platelet levels were significantly decreased. The increases in platelet activity and its related procoagulant activity appear to account in part for the hypercoagulability observed in diabetes mellitus. Our findings suggest that activated platelets might play a role in the development of diabetic nephropathy. Furthermore, antiplatelet therapy with cilostazol for diabetic patients may be useful as antithrombin therapy including antiplatelet therapy, since it suppresses the production of intrinsic coagulants produced by platelet activation.

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“…To quantify fluorescence, the mean fluorescence intensity (MFI) was determined as MFI = (mean of the positive region) ! (percentage of positivity) [31].…”

Section: Flow Cytometry Of Aggregatesmentioning

confidence: 99%

Morphological Differences between GPIb Antibody-Induced and Shear Stress-Induced Platelet Aggregates

Nomura

Tandon

Nakamura

et al. 2000

Pathophysiol Haemos Thromb

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We determined the morphological differences between NNKY5-5-induced and shear stress-induced platelet aggregates using confocal laser scanning microscopy, and investigated the effects of cytokines on these aggregates. As shear stress was increased from low to high, many aggregates were generated. Some platelets and aggregates already exhibited PAC-1 binding at low shear stress. And finally, aggregates were clearly stained by PAC-1 at high shear stress. Low shear stress-induced aggregates included fibrinogen, but not von Willebrand factor (vWF). In contrast, high shear stress-induced aggregates included both fibrinogen and vWF. NNKY5-5-induced platelet aggregates exhibited both PAC-1 binding and fibrinogen, but vWF was not found in them. The aggregate formation in NNKY5-5-treated platelet-rich plasma (PRP) at low shear stress was clearly more pronounced than that in untreated PRP. In addition, aggregates in NNKY5-5-treated PRP formed after 6 min under low shear stress were clearly stained by PAC-1 and included fibrinogen, but vWF was not found in them. In order to investigate the effects of cytokines on platelet activation under NNKY5-5 stimulation, changes in light transmission and light scatter were assessed with an AG-10. G-CSF, IL-6 and thrombopoietin (TPO) each enhanced light scatter compared to control PRP, although IL-3, GM-CSF, erythropoietin and stem cell factor did not. In particular, TPO significantly enhanced the ‘%-T’ and ‘S-Max’ of NNKY5-5-treated PRP. TPO clearly enhanced the aggregate formation with high shear stress or NNKY5-5 stimulation. These results suggest that the glycoprotein Ib (GPIb)-unmediated aggregates can be formed with only fibrinogen, but the GPIb-mediated aggregates by vWF and fibrinogen synergistically. In particular, the latter is formed more firmly, and both aggregates are enhanced by TPO.

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Differences between platelet and microparticle glycoprotein IIb/IIIa

Cited by 53 publications

References 40 publications

Detection of monocyte-derived microparticles in patients with Type II diabetes mellitus

Detection of monocyte-derived microparticles in patients with Type II diabetes mellitus

Significance of Platelet-Derived Microparticles and Activated Platelets in Diabetic Nephropathy

Morphological Differences between GPIb Antibody-Induced and Shear Stress-Induced Platelet Aggregates

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