Differences between healthy hematopoietic progenitors and leukemia cells with respect to CD44 mediated rolling versus adherence behavior on hyaluronic acid coated surfaces

Hanke, Maximilian; Hoffmann, Isabel; Christophis, Christof; Schubert, Mario; Hoang, Van T.; Zepeda‐Moreno, Abraham; Baran, Natalia; Eckstein, Volker; Wuchter, Patrick; Rosenhahn, Axel; Ho, Anthony D.

doi:10.1016/j.biomaterials.2013.11.011

Cited by 22 publications

(17 citation statements)

References 33 publications

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“…17,27,28 Similarly, multiple reports have already described altered expression of adhesion receptors (e.g., CXCR4, CD44, VLA-4, CD166) in AML cells 29,30 as well as differences in interaction with bone marrow components between primary AML blasts and normal progenitor cells. 31 Targeting of cellmatrix interactions is considered to be a promising tool for eradication of the residual disease in AML. In B-cell chronic lymphocytic leukemia (CLL), increased levels of cortactin, which is involved in regulation of cell motility, was found to correlate with negative prognostic factors.…”

Section: Discussionmentioning

confidence: 99%

Adhesion structures in leukemia cells and their regulation by Src family kinases

Röselová

Obr

Holoubek

et al. 2017

Cell Adhesion & Migration

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Interaction of leukemia blasts with the bone marrow extracellular matrix often results in protection of leukemia cells from chemotherapy and in persistence of the residual disease which is on the basis of subsequent relapses. The adhesion signaling pathways have been extensively studied in adherent cells as well as in mature haematopoietic cells, but the adhesion structures and signaling in haematopoietic stem and progenitor cells, either normal or malignant, are much less explored. We analyzed the interaction of leukemia cells with fibronectin (FN) using interference reflection microscopy, immunofluorescence, measurement of adherent cell fraction, real-time microimpedance measurement and live cell imaging. We found that leukemia cells form very dynamic adhesion structures similar to early stages of focal adhesions. In contrast to adherent cells, where Src family kinases (SFK) belong to important regulators of focal adhesion dynamics, we observed only minor effects of SFK inhibitor dasatinib on leukemia cell binding to FN. The relatively weak involvement of SFK in adhesion structure regulation might be associated with the lack of cytoskeletal mechanical tension in leukemia cells. On the other hand, active Lyn kinase was found to specifically localize to leukemia cell adhesion structures and a less firm cell attachment to FN was often associated with higher Lyn activity (this unexpectedly occurred also after cell treatment with the inhibitor SKI-1). Lyn thus may be important for signaling from integrin-associated complexes to other processes in leukemia cells.

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Section: Discussionmentioning

confidence: 99%

Adhesion structures in leukemia cells and their regulation by Src family kinases

Röselová

Obr

Holoubek

et al. 2017

Cell Adhesion & Migration

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“…Recent applications of microfluidic removal assays include fibroblast adhesion to differently hydrated ethylene-glycol (EG) self-assembled monolayers (SAMs) (Christophis et al 2010), the removal of cells from anisotropic polymeric nanofilms (Christophis, Sekeroglu, et al 2011), removal of marine bacteria from self-assembled monolayers (Arpa-Sancet et al 2012), and removal of germlings of macroalgae (Dimartino et al 2015). Microfluidic assays have also been applied in leukemia research to correlate the rolling behavior of leukemic blast cells of different patients, with therapeutic success (Christophis, Taubert, et al 2011;Hanke et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Microfluidic detachment assay to probe the adhesion strength of diatoms

Alles

Rosenhahn

2015

Biofouling

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Fouling release (FR) coatings are increasingly applied as an environmentally benign alternative for controlling marine biofouling. As the technology relies on removing fouling by water currents created by the motion of ships, weakening of adhesion of adherent organisms is the key design goal for improved coatings. In this paper, a microfluidic shear force assay is used to quantify how easily diatoms can be removed from surfaces. The experimental setup and the optimization of the experimental parameters to study the adhesion of the diatom Navicula perminuta are described. As examples of how varying the physico-chemical surface properties affects the ability of diatoms to bind to surfaces, a range of hydrophilic and hydrophobic self-assembled monolayers was compared. While the number of cells that attached (adhered) was barely affected by the coatings, the critical shear stress required for their removal from the surface varied significantly.

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“…HA-CD44 interactions have been shown to be necessary for the capture and rolling of subsets of CD44+ T-lymphocytes [9], and the firm adhesion of CD44+ neutrophils on the blood vessel endothelium [10,11] in response to inflammatory stimuli in vivo. In addition to leukocytes [9,[12][13][14], stem cells [15][16][17] and cancer cells [16,18,19] employ HA-CD44 binding for cell adhesion, as demonstrated by in vitro assays utilising CD44+ cells and soluble HA or immobilised HA/HA-expressing endothelial monolayers under static conditions or inside flow channels, which mimic the physiological shear stress of post-capillary venules (~1-4 dyn/cm 2 [20]). Such flow assays have also revealed that HA-CD44-mediated adhesion is tightly regulated, by mechanisms including CD44 remodelling [21] under activating conditions [13,22,23], and changes to HA architecture in response to inflammatory effectors [5,[23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

An integrated assay to probe endothelial glycocalyx-blood cell interactions under flow in mechanically and biochemically well-defined environments

et al. 2019

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Cell-cell and cell-glycocalyx interactions under flow are important for the behaviour of circulating cells in blood and lymphatic vessels. However, such interactions are not well understood due in part to a lack of tools to study them in defined environments. Here, we develop a versatile in vitro platform for the study of cell-glycocalyx interactions in well-defined physical and chemical settings under flow. Our approach is demonstrated with the interaction between hyaluronan (HA, a key component of the endothelial glycocalyx) and its cell receptor CD44. We generate HA brushes in situ within a microfluidic device, and demonstrate the tuning of their physical (thickness and softness) and chemical (density of CD44 binding sites) properties using characterisation with reflection interference contrast microscopy (RICM) and application of polymer theory. We highlight the interactions of HA brushes with CD44-displaying beads and cells under flow. Observations of CD44+ beads on a HA brush with RICM enabled the 3-dimensional trajectories to be generated, and revealed interactions in the form of stop and go phases with reduced rolling velocity and reduced distance between the bead and the HA brush, compared to uncoated beads. Combined RICM and bright-field microscopy of CD44+ AKR1 T-lymphocytes revealed complementary information about the dynamics of cell rolling and cell morphology, and highlighted the formation of tethers and slings, as they interacted with a HA brush under flow. This platform can readily incorporate more complex models of the glycocalyx, and should permit the study of how mechanical and biochemical factors are orchestrated to enable highly selective blood cell-vessel wall interactions under flow.

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Differences between healthy hematopoietic progenitors and leukemia cells with respect to CD44 mediated rolling versus adherence behavior on hyaluronic acid coated surfaces

Cited by 22 publications

References 33 publications

Adhesion structures in leukemia cells and their regulation by Src family kinases

Adhesion structures in leukemia cells and their regulation by Src family kinases

Microfluidic detachment assay to probe the adhesion strength of diatoms

An integrated assay to probe endothelial glycocalyx-blood cell interactions under flow in mechanically and biochemically well-defined environments

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