Differences between collagen morphologies, properties and distribution in diabetic and normal biobreeding and Sprague–Dawley rat sciatic nerves

Layton, Bradley E.; Sastry, Ann Marie; Wang, H.; Sullivan, Kelli A.; Feldman, Eva L.; Komorowski, Thomas E.; Philbert, Martin A.

doi:10.1016/j.jbiomech.2003.11.008

Cited by 30 publications

(29 citation statements)

References 52 publications

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“…Though we expect glucose-induced crosslinking to produce classically altered mechanical response in collagenreinforced tissue, our prior work [18] showed no significant differences in mechanical response between diabetic and control tissues up to 40% strain. This strain is nearly coincident with the strain required to ''straighten'' collagen fibers, which exhibit significant waviness; we may presume that after this point, the mechanics of the actual crosslinks are investigated.…”

Section: Modeling and Hypothesesmentioning

confidence: 73%

“…This tissue undergoes glycation [17,18], and exhibits altered mechanical properties, which can be modeled using micromechanics [18,19]. The gross mechanical properties of peripheral nerve [20,21] and collagen fibrils [22,23] have been quantified, but until our own work, there was apparently no model correlating glycation with mechanical properties at the tissue scale.…”

Section: Introductionmentioning

confidence: 99%

“…The gross mechanical properties of peripheral nerve [20,21] and collagen fibrils [22,23] have been quantified, but until our own work, there was apparently no model correlating glycation with mechanical properties at the tissue scale. Our methodology [18,19] was an application of classical bundle theory, applied to the fibrillar collagen of the nerve sheath [24][25][26]. The model was developed for the fibril scale ( Fig.…”

Section: Introductionmentioning

confidence: 99%

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Equal and local-load-sharing micromechanical models for collagens: Quantitative comparisons in response of non-diabetic and diabetic rat tissue

Layton

Sastry

2006

Acta Biomaterialia

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Section: Modeling and Hypothesesmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Equal and local-load-sharing micromechanical models for collagens: Quantitative comparisons in response of non-diabetic and diabetic rat tissue

Layton

Sastry

2006

Acta Biomaterialia

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“…The inner connective tissue layer is the endoneurium that encloses a nerve fiber with its axon and covering Schwann cell (1). Diabetic neuropathy is associated with changes in the extracellular matrix (ECM) and the basement membrane thickening in peripheral nerves (2)(3)(4). The ECM provides physical support for cells and tissues and also has a major role in proliferation, differentiation, migration and regulating cell behavior (5).…”

Section: Introductionmentioning

confidence: 99%

Effects of Alpha Lipoic Acid Treatment on Laminin Alteration of the Sciatic Nerve in Streptozotocin Induced Diabetic Rats

Aldaghi

Sankian

Sameni

et al. 2014

Middle East J Rehabil Health

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Background: Neuropathy is one of the most complications of diabetes that may be associated with changes in the structure and function of the basement membranes in nerves. Laminin is one of the most important components of basement membranes in sciatic nerve. Objectives: In this study, the protective effects of Alpha lipoic acid (ALA) as an antioxidant agent on blood glucose level, body weight and laminin expression in sciatic nerve of diabetic rats has been evaluated. Materials and Methods: 24 adult male rats were randomly divided into 3 groups: control, untreated diabetic and diabetic treated with ALA. Diabetes was induced in the rats by a single dose injection of streptozotocin (55 mg/kg). 12 weeks after treatment, body weight and blood glucose levels were measured and then rats were anesthetized and sciatic nerves were removed and alterations in laminin expression has been evaluated by immunohistochemical study and Real time PCR method. Statistical evaluation was carried out by using one way ANOVA followed by Tukey test. Results: Untreated diabetic rats showed a significant increase in laminin expression in the perineurium, around the Schwann cells and blood vessels walls of the sciatic nerves (P < 0.05). ALA treatment did not affect on body weight, but significantly decreased blood glucose levels (P < 0.05). ALA treatment significantly decreased laminin expression only in the blood vessels walls of sciatic nerves (P < 0.05). Laminin β1 mRNA level in sciatic nerve of untreated diabetic rats increased 0.6 fold compared to control rats (P < 0.05). Although ALA decreased this up regulation but it was not significant. Conclusions:The results indicated that untreated diabetic neuropathy in rat might be associated with increased laminin expression in sciatic nerve and also it may be assumed that ALA treatment protected the blood vessels walls of nerves against laminin over expression in STZ induced diabetic rats.

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“…Diabetic neuropathy is associated with changes in the Extracellular Matrix (ECM) and the basement membrane thickening in peripheral nerves (Bradley et al, 2000;Yasuda et al, 2003;Layton et al, 2004). The ECM provides physical support for cells and tissues and also has a major role in proliferation, differentiation, migration and regulating cell behavior (Yasuda et al, 2003;Layton et al, 2004;Chernousov et al, 2008;Miner, 2008). Basement membranes are structures of ECM that cover the basal side of epithelial and endothelial cells (Miner, 2008).…”

Section: Introductionmentioning

confidence: 99%

Comparison of insulin and alpha lipoic acid treatment on laminin expression in sciatic nerve of diabetic rats

Aldaghi¹

2013

J. Cell Anim. Biol.

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Diabetic neuropathy is associated with changes in composition of extracellular matrix. Laminin is a major component of extracellular matrix. In this study, we investigated protective effects of insulin and alpha lipoic acid on improving of blood glucose levels, body weight and laminin expression of sciatic nerve in diabetic rats. Diabetes was induced in the rats by 55 mg/kg streptozotocin (STZ) via an intraperitoneal injection. 12 weeks after STZ treatment, laminin expression in sciatic nerves was evaluated by immunohistochemical study and real time polymerase chain reaction. Laminin expression increased in the perineurium, endoneurium and blood vessels walls in sciatic nerve of untreated diabetic rats compared to control rats. Insulin treatment significantly reduced overall laminin immunoreactivity but alpha lipoic acid treatment significantly decreased it only in the blood vessels walls. Laminin β1 mRNA level in sciatic nerve of untreated diabetic group increased 0.6 fold compared to control group. Although, alpha lipoic acid decreased this up regulation but unlike insulin, this reduction was not significant. These findings suggest that untreated diabetic neuropathy in rats might be associated with increased laminin expression and also it implies that insulin therapy is more effective than alpha lipoic acid treatment in improving hyperglycemia and laminin up-regulation in diabetic nerves.

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Differences between collagen morphologies, properties and distribution in diabetic and normal biobreeding and Sprague–Dawley rat sciatic nerves

Cited by 30 publications

References 52 publications

Equal and local-load-sharing micromechanical models for collagens: Quantitative comparisons in response of non-diabetic and diabetic rat tissue

Equal and local-load-sharing micromechanical models for collagens: Quantitative comparisons in response of non-diabetic and diabetic rat tissue

Effects of Alpha Lipoic Acid Treatment on Laminin Alteration of the Sciatic Nerve in Streptozotocin Induced Diabetic Rats

Comparison of insulin and alpha lipoic acid treatment on laminin expression in sciatic nerve of diabetic rats

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