Differences between blood cells of juvenile and adult specimens of the pond snail Lymnaea stagnalis

Dikkeboom, Ronald; Knaap, W.P.W. van der; Meuleman, Elisabeth A.; Sminia, T.

doi:10.1007/bf00215142

Cited by 48 publications

(18 citation statements)

References 18 publications

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“…Actually, some mild variations did occur in hemocyte counting, but this applied more to the size of the snail (shell diameter), rather than to the presence of schistosome infection. Regarding this latter finding our data only confirm those of Stumpf and Gilbertson (1978), and Dikkeboom et al (1984). The shell size is related to the age of the snail, and probably the variation in hemocyte concentration in the hemolymph may be under the influence of several factors, among them sexual maturity (Sminia et al 1974).…”

Section: Discussioncontrasting

confidence: 74%

A contribution to the pathobiology of Biomphalaria glabrata hemocytes

Borges

Azevedo

Andrade

2006

Mem. Inst. Oswaldo Cruz

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Section: Discussioncontrasting

confidence: 74%

A contribution to the pathobiology of Biomphalaria glabrata hemocytes

Borges

Azevedo

Andrade

2006

Mem. Inst. Oswaldo Cruz

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“…Lymnaea stagnalis to Trichobilharzia ocellata [14]. Also, hemocytes of juvenile L. stagnalis were shown to be less differentiated in morphology and less active in a lysosomal enzyme, peroxidase, than those of adults [1]. Based on these findings, the present study was done to confirm that juvenile C. retropictus could preserve TDH-producing strains at higher levels than adults and that juveniles could preserve TDH-producing strains at higher levels than TDHnon-producing strains.…”

mentioning

confidence: 64%

Selective Survival of a Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus in the Alimentary Tract of a Juvenile Estuarine Gastropod(Clithon retropictus).

Kumazawa

Kawasaki

1997

The Journal of Veterinary Medical Science

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ABSTRACT. Juvenile estuarine gastropods (Clithon retropictus), maintained in ultraviolet ray-irradiated recirculating artificial seawater with a salinity of 20 at 28°C, preserved thermostable direct hemolysin (TDH)-producing strain D-3 of Vibrio parahaemolyticus at a level of 10 4 -10 5 colony forming units per gram (cfu/g) and TDH-non-producing strains N-18 and R-13 at a level of 10 1 -10 2 cfu/g in the alimentary tract for at least 21 days after ingestion. In adults, the numbers of the three strains decreased to a level of 10 0 cfu/g within 21 days under the same conditions. This evidence supports our recent observations that TDH-producing strains increased to a high level in the summer months in the presence of high levels of TDH-non-producing strains in the alimentary tract of juvenile C. retropictus at estuaries in Japan. Lymnaea stagnalis to Trichobilharzia ocellata [14]. Also, hemocytes of juvenile L. stagnalis were shown to be less differentiated in morphology and less active in a lysosomal enzyme, peroxidase, than those of adults [1]. Based on these findings, the present study was done to confirm that juvenile C. retropictus could preserve TDH-producing strains at higher levels than adults and that juveniles could preserve TDH-producing strains at higher levels than TDHnon-producing strains. MATERIALS AND METHODSGastropod: Adult and juvenile C. retropictus, 3.0-4.8 g and 0.2-0.9 g weight, respectively, were maintained in aquaria with tap water at 28°C for one week and confirmed non-detectable for V. parahaemolyticus before experiments.Bacteria: V. parahaemolyticus strains D-3 (TDHp r o d u c i n g ; n a l i d i x i c a c i d -r e s i s t a n t ) , N -1 8 (TDH-non-producing; nalidixic acid-resistant) and R-13 (TDH-non-producing; rifampicin-resistant) [9] were grown in Trypticase Soy Broth (Difco, U.S.A.) containing 1% NaCl at 37°C for 8 hr with shaking. The cells were washed with 20 artificial seawater [9] and suspended at approximately 10 6 cfu/ml in the artificial seawater. Inoculation: A hundred gastropods were incubated in 100 ml of the bacterial suspension containing a single strain in 20 artificial seawater at 25°C for 24 hr with aeration. Then they were washed with distilled water to remove the strains attached to the surface and maintained in aquaria with 30 l of UV-irradiated, recirculating, 20 artificial seawater at 28°C for up to 21 days. Cultured diatom algae, Navicula strain F-1 [2], were fed to the gastropods throughout the study. Every 10-15 gastropods were sacrificed at appropriate time intervals to obtain homogenates of the alimentary tracts attached to mid-gut Thermostable-direct hemolysin(TDH)-producing strain of Vibrio parahaemolyticus is a potential pathogen of human gastroenteritis. The strain was detected at the highest level of 10 5 colony forming units per gram (cfu/g) from a neritid gastropod (Clithon retropictus) at a site of the Hashizu estuary in Japan where juveniles, less than 1.0 g weight, were predominant in a C. retropictus community. It was not, however, detected at sit...

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“…infection, 14,15 as enhanced trematode cercarial development has been shown to occur in juvenile snails because they have a weaker defense system. [34][35][36] In addition, O. viverrini s.l. may be more pathogenic to snails than the virgulate species leading to higher death rate of larger infected snails.…”

Section: Discussionmentioning

confidence: 99%

Seasonal Transmission of Opisthorchis viverrini sensu lato and a Lecithodendriid Trematode Species in Bithynia siamensis goniomphalos Snails in Northeast Thailand

Namsanor¹,

Sithithaworn²,

Kopolrat³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. Seasonal changes play roles in the transmission success of fish-borne zoonotic trematodes (FZT). This study examined the seasonal transmission patterns of Opisthorchis viverrini sensu lato (s.l.) and a virgulate cercaria (family Lecithodendriidae) in the snail intermediate host, Bithynia siamensis goniomphalos in northeast Thailand. Snail samples were collected monthly during the rainy, cool, and hot seasons during 2012-2013 to determine the prevalence and intensity of larval trematode infections. The prevalence of O. viverrini s.l. varied significantly with season, being 0.31%, 1.05%, and 0.37% in the rainy, cool, and hot seasons, respectively (P 0.05). Similarly, the prevalence of virgulate cercariae was 3.11%, 6.80%, and 1.64% in the rainy, cool, and hot seasons, respectively (P 0.05). The intensity of larval trematode infections also varied between seasons and peaked in the hot season (P 0.05) in both species. The snails infected with O. viverrini s.l. were significantly smaller (P 0.05) and those infected with virgulate cercariae were significantly larger (P 0.05) than uninfected snails. Seasonal variation and the different sizes of B. s. goniomphalos parasitized by O. viverrini s.l. and virgulate trematodes indicate complex host-parasite interactions with important implications for the epidemiology of O. viverrini s.l.

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Differences between blood cells of juvenile and adult specimens of the pond snail Lymnaea stagnalis

Cited by 48 publications

References 18 publications

A contribution to the pathobiology of Biomphalaria glabrata hemocytes

A contribution to the pathobiology of Biomphalaria glabrata hemocytes

Selective Survival of a Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus in the Alimentary Tract of a Juvenile Estuarine Gastropod(Clithon retropictus).

Seasonal Transmission of Opisthorchis viverrini sensu lato and a Lecithodendriid Trematode Species in Bithynia siamensis goniomphalos Snails in Northeast Thailand

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