Differences Between Bacterial Communities Associated with the Surface or Tissue of Mediterranean Sponge Species

Gerçe, Berna; Syldatk, Christoph; Hausmann, Rudolf

doi:10.1007/s00248-011-9802-2

Cited by 32 publications

(23 citation statements)

References 47 publications

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“…Seven OTUs were connected to both A. oroides and Chondrosia reniformis among which the most abundant (OTU17) was identified as a Chloroflexi . This OTU has also been found in C. reniformis in a previous study (clone CrT‐1, Gerçe et al ., ). Six OTUs connected Petrosia ficiformis to C. reniformis with a Nitrospirales being the most abundant bacteria (OTU72).…”

Section: Resultsmentioning

confidence: 97%

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Removing environmental sources of variation to gain insight on symbionts vs. transient microbes in high and low microbial abundance sponges

Blanquer

Uriz

Galand

2013

Environmental Microbiology

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In this study, we pursue unravelling the bacterial communities of 26 sponges, belonging to several taxonomical orders, and comprising low microbial abundance (LMA) and high microbial abundance (HMA) representatives. Particularly, we searched for species-specific bacteria, which could be considered as symbionts. To reduce temporal and spatial environmentally caused differences between host species, we sampled all the sponge species present in an isolated small rocky area in a single dive. The bacterial communities identified by pyrosequencing the 16S rRNA gene showed that all HMA species clustered separated from LMA sponges and seawater. HMA sponges often had highest diversity, but some LMA sponges had also very diverse bacterial communities. Network analyses indicated that no core bacterial community seemed to exist for the studied sponges, not even for such a space and time-restricted sampling. Most sequences, particularly the most abundant ones in each species, were species-specific for both HMA and LMA sponges. The bacterial sequences retrieved from LMA sponges, despite being phylogenetically more similar to seawater, did not represent transient seawater bacteria. We conclude that sponge bacterial communities depend more on the host affiliation to the HMA or LMA groups than on host phylogeny.

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Section: Resultsmentioning

confidence: 97%

“…In contrast, the Alphaproteobacteria SAR116 cluster represented almost half of all sequences from LMA sponge bacteria. Thus, the Alphaproteobacteria present in our LMA sponges were not the result of a temporary enrichment of seawater bacteria, as suggested previously (Weisz et al ., ; Gerçe et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Removing environmental sources of variation to gain insight on symbionts vs. transient microbes in high and low microbial abundance sponges

Blanquer

Uriz

Galand

2013

Environmental Microbiology

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“…consistently displayed less complex banding patterns than their HMA counterparts Gerce et al, 2011;Poppell et al, 2013). Even small 16S rRNA gene clone libraries were sufficient to detect the major phylogenetic lineages (Moitinho-Silva and Hentschel, unpubl.…”

Section: Resultsmentioning

confidence: 99%

The HMA-LMA Dichotomy Revisited: an Electron Microscopical Survey of 56 Sponge Species

Gloeckner¹,

Wehrl²,

Moitinho‐Silva³

et al. 2014

The Biological Bulletin

196

206

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Abstract. The dichotomy between high microbial abundance (HMA) and low microbial abundance (LMA) sponges has been long recognized. In the present study, 56 sponge species from three geographic regions (greater Caribbean, Mediterranean, Red Sea) were investigated by transmission electron microscopy for the presence of microorganisms in the mesohyl matrix. Additionally, bacterial enumeration by DAPI-counting was performed on a subset of samples. Of the 56 species investigated, 28 were identified as belonging to the HMA and 28 to the LMA category. The sponge orders Agelasida and Verongida consisted exclusively of HMA species, and the Poecilosclerida were composed only of LMA sponges. Other taxa contained both types of microbial associations (e.g., marine Haplosclerida, Homoscleromorpha, Dictyoceratida), and a clear phylogenetic pattern could not be identified. For a few sponge species, an intermediate microbial load was determined, and the microscopy data did not suffice to reliably determine HMA or LMA status. To experimentally determine the HMA or LMA status of a sponge species, we therefore recommend a combination of transmission electron microscopy and 16S rRNA gene sequence data. This study significantly expands previous reports on microbial abundances in sponge tissues and contributes to a better understanding of the HMA-LMA dichotomy in sponge-microbe symbioses.

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“…The selection of habitats was based on evidence provided by previous studies on the potential richness of chitinolytic bacterial species and diverse enzymatic activities (Chaston and Goodrich-Blair 2010; Gerce et al 2011; Hjort et al 2010; Kennedy et al 2007; Manucharova et al 2006; Metcalfe et al 2002a; Sjoling et al 2007; Terahara et al 2009; Williamson et al 2000). …”

Section: Methodsmentioning

confidence: 99%

Mining of unexplored habitats for novel chitinases—chiA as a helper gene proxy in metagenomics

Cretoiu

Kielak

Al‐Soud

et al. 2012

Appl Microbiol Biotechnol

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The main objective of this study was to assess the abundance and diversity of chitin-degrading microbial communities in ten terrestrial and aquatic habitats in order to provide guidance to the subsequent exploration of such environments for novel chitinolytic enzymes. A combined protocol which encompassed (1) classical overall enzymatic assays, (2) chiA gene abundance measurement by qPCR, (3) chiA gene pyrosequencing, and (4) chiA gene-based PCR-DGGE was used. The chiA gene pyrosequencing is unprecedented, as it is the first massive parallel sequencing of this gene. The data obtained showed the existence across habitats of core bacterial communities responsible for chitin assimilation irrespective of ecosystem origin. Conversely, there were habitat-specific differences. In addition, a suite of sequences were obtained that are as yet unregistered in the chitinase database. In terms of chiA gene abundance and diversity, typical low-abundance/diversity versus high-abundance/diversity habitats was distinguished. From the combined data, we selected chitin-amended agricultural soil, the rhizosphere of the Arctic plant Oxyria digyna and the freshwater sponge Ephydatia fluviatilis as the most promising habitats for subsequent bioexploration. Thus, the screening strategy used is proposed as a guide for further metagenomics-based exploration of the selected habitats.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-012-4057-5) contains supplementary material, which is available to authorized users.

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Differences Between Bacterial Communities Associated with the Surface or Tissue of Mediterranean Sponge Species

Cited by 32 publications

References 47 publications

Removing environmental sources of variation to gain insight on symbionts vs. transient microbes in high and low microbial abundance sponges

Removing environmental sources of variation to gain insight on symbionts vs. transient microbes in high and low microbial abundance sponges

The HMA-LMA Dichotomy Revisited: an Electron Microscopical Survey of 56 Sponge Species

Mining of unexplored habitats for novel chitinases—chiA as a helper gene proxy in metagenomics

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